Restriction map for pF1KE0116
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AviII       BamHI       BbeI        BbrPI       
BbvCI       BciVI       BclI        BfrI        BglII       
BlpI        Bpu1102I    BsaAI       BsaBI       BsaI        
BseAI       BsiEI       BsiWI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO109I    
EcoO65I     FbaI        FseI        FspI        HincII      
HindII      HpaI        KasI        KspI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SwaI        TspRI       Tth111I     XbaI        XcmI        
XhoI        XmnI        


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# ONE CUT ENZYMES (   63 enzymes) #
###################################

Acc65I        608  GGTACC               G^GTACC
AccII          11  CGCG                 CG^CG
AcyI          958  GACGCC               G[GA]^CG[CT]C
Alw44I         37  GTGCAC               G^TGCAC
AlwNI         327  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          475  CCTGAGG              CC^T[ACGT]AGG
ApaLI          37  GTGCAC               G^TGCAC
Asp718I       608  GGTACC               G^GTACC
AvrII         381  CCTAGG               C^CTAGG
BalI          539  TGGCCA               TGG^CCA
BanI          608  GGTACC               G^G[CT][GA]CC
BanII         973  GAGCTC               G[GA]GC[CT]^C
BcgI         1136  CGAAGCCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI          381  CCTAGG               C^CTAGG
BsaHI         958  GACGCC               G[GA]^CG[CT]C
BsaWI        1025  ACCGGA               [AT]^CCGG[AT]
BsgI          526  GTGCAG               GTGCAG(16/14)
BsmBI         718  GAGACG               CGTCTC(1/5)
Bsp1407I      461  TGTACA               T^GTACA
BsrFI         605  GCCGGT               [GA]^CCGG[CT]
BsrGI         461  TGTACA               T^GTACA
BssSI         274  CACGAG               CACGAG(-5/-1)
BstAPI        528  GCAGGACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI          11  CGCG                 CG^CG
BstXI         168  CCACTCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         860  AGATCC               [GA]^GATC[CT]
Bsu36I        475  CCTGAGG              CC^T[ACGT]AGG
CfoI          702  GCGC                 GCG^C
Cfr10I        605  GCCGGT               [GA]^CCGG[CT]
DsaI          155  CCATGG               C^C[GA][CT]GG
EarI          297  GAAGAG               CTCTTC(1/4)
Eco81I        475  CCTGAGG              CC^T[ACGT]AGG
EcoNI         828  CCTGGAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         930  GAATTC               G^AATTC
EcoRV        1116  GATATC               GAT^ATC
EcoT22I      1018  ATGCAT               ATGCA^T
HaeII         701  GGCGCT               [GA]GCGC^[CT]
HhaI          702  GCGC                 GCG^C
Hin1I         958  GACGCC               G[GA]^CG[CT]C
HinP1I        702  GCGC                 G^CGC
HphI           94  GGTGA                GGTGA(8/7)
KpnI          608  GGTACC               GGTAC^C
Ksp632I       297  GAAGAG               CTCTTC(1/4)
MaeII          35  ACGT                 A^CGT
MaeIII        595  GTCAC                ^GT[ACGT]AC
MflI          860  AGATCC               [GA]^GATC[CT]
MluNI         539  TGGCCA               TGG^CCA
MscI          539  TGGCCA               TGG^CCA
MvnI           11  CGCG                 CG^CG
NcoI          155  CCATGG               C^CATGG
NsiI         1018  ATGCAT               ATGCA^T
NspI          878  ACATGC               [GA]CATG^[CT]
PflMI         864  CCACCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          694  CTGCAG               CTGCA^G
SacI          973  GAGCTC               GAGCT^C
SspBI         461  TGTACA               T^GTACA
SspI          283  AATATT               AAT^ATT
StuI          731  AGGCCT               AGG^CCT
TaqI          399  TCGA                 T^CGA
Tsp45I        595  GTCAC                ^GT[GC]AC
TthHB8I       399  TCGA                 T^CGA
Van91I        864  CCACCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         860  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcsI          930  GAATTC               [GA]^AATT[CT]
             1008  GAATTT              
AlwI          861  GATCC                GGATC(4/5)
              945  GATCC               
ApoI          930  GAATTC               [GA]^AATT[CT]
             1008  GAATTT              
AspHI          37  GTGCAC               G[AT]GC[AT]^C
              973  GAGCTC              
AvaI         1082  CCCGGG               C^[CT]CG[GA]G
             1101  CCCGGG              
AvaII          48  GGACC                G^G[AT]CC
              306  GGTCC               
BbsI          144  GAAGAC               GAAGAC(2/6)
              519  GAAGAC              
BglI          127  GCCCAGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              733  GCCTTACTGGC         
BmyI           37  GTGCAC               G[AGT]GC[ACT]^C
              973  GAGCTC              
BpuAI         144  GAAGAC               GAAGAC(2/6)
              519  GAAGAC              
BseRI         223  GAGGAG               GAGGAG(10/8)
              652  GAGGAG              
BsiHKAI        37  GTGCAC               G[AT]GC[AT]^C
              973  GAGCTC              
BsmAI         260  GAGAC                GTCTC(1/5)
              718  GAGAC               
BsoBI        1082  CCCGGG               C^[CT]CG[GA]G
             1101  CCCGGG              
Bsp1286I       37  GTGCAC               G[AGT]GC[ACT]^C
              973  GAGCTC              
EaeI          539  TGGCCA               [CT]^GGCC[GA]
              842  TGGCCG              
EcoT14I       155  CCATGG               C^C[AT][AT]GG
              381  CCTAGG              
HgaI            9  GACGC                GACGC(5/10)
              958  GACGC               
HindIII       367  AAGCTT               A^AGCTT
              544  AAGCTT              
MseI          233  TTAA                 T^TAA
              287  TTAA                
NlaIV         377  GGAACC               GG[ACGT]^[ACGT]CC
              608  GGTACC              
PvuII         173  CAGCTG               CAG^CTG
              292  CAGCTG              
SfaNI        1017  GATGC                GCATC(5/9)
              726  GCATC               
SfcI          337  CTACAG               C^T[GA][CT]AG
              694  CTGCAG              
SmaI         1082  CCCGGG               CCC^GGG
             1101  CCCGGG              
StyI          155  CCATGG               C^C[AT][AT]GG
              381  CCTAGG              
TfiI          270  GATTC                G^A[AT]TC
              388  GATTC               
Tru9I         233  TTAA                 T^TAA
              287  TTAA                
Tsp509I       931  AATT                 ^AATT
             1009  AATT                
XmaCI        1082  CCCGGG               C^CCGGG
             1101  CCCGGG              
XmaI         1082  CCCGGG               C^CCGGG
             1101  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BcnI        
BfaI        BpmI        BsaJI       BsiYI       BslI        
BsmFI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       ItaI        MaeI        MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SmlI        TseI        
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