Restriction map for pF1KE0150
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BalI        
BamHI       BanII       BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstYI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     KspI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MunI        NciI        NcoI        NdeI        
NheI        NotI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TspRI       Tth111I     Van91I      XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcsI          916  GAATTC               [GA]^AATT[CT]
AocI          481  CCTGAGG              CC^T[ACGT]AGG
ApoI          916  GAATTC               [GA]^AATT[CT]
AvaI          175  CCCGAG               C^[CT]CG[GA]G
BbsI          740  GAAGAC               GAAGAC(2/6)
BfaI          436  CTAG                 C^TAG
BpuAI         740  GAAGAC               GAAGAC(2/6)
BseRI         484  GAGGAG               GAGGAG(10/8)
BsgI          148  GTGCAG               GTGCAG(16/14)
BsmAI         670  GTCTC                GTCTC(1/5)
BsoBI         175  CCCGAG               C^[CT]CG[GA]G
BspMI         144  GCAGGT               ACCTGC(4/8)
BsrBI         124  GAGCGG               CCGCTC(-3/-3)
BsrDI         681  CATTGC               GCAATG(2/0)
BsrFI          76  GCCGGC               [GA]^CCGG[CT]
BstXI         558  CCAAGGGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        481  CCTGAGG              CC^T[ACGT]AGG
Cfr10I         76  GCCGGC               [GA]^CCGG[CT]
DpnII         168  GATC                 ^GATC
EaeI           74  TGGCCG               [CT]^GGCC[GA]
Eco81I        481  CCTGAGG              CC^T[ACGT]AGG
EcoRI         916  GAATTC               G^AATTC
EcoT14I       558  CCAAGG               C^C[AT][AT]GG
EcoT22I         1  ATGCAT               ATGCA^T
FauI          126  GCGGG                CCCGC(4/6)
HaeIII         75  GGCC                 GG^CC
HapII          77  CCGG                 C^CGG
HpaII          77  CCGG                 C^CGG
MaeI          436  CTAG                 C^TAG
MaeII         744  ACGT                 A^CGT
MboI          168  GATC                 ^GATC
MspI           77  CCGG                 C^CGG
NaeI           76  GCCGGC               GCC^GGC
NdeII         168  GATC                 ^GATC
NgoMIV         76  GCCGGC               G^CCGGC
NruI          222  TCGCGA               TCG^CGA
NsiI            1  ATGCAT               ATGCA^T
NspI          700  ACATGC               [GA]CATG^[CT]
PleI           83  GAGTC                GAGTC(4/5)
PstI          142  CTGCAG               CTGCA^G
PvuII          92  CAGCTG               CAG^CTG
Sau3AI        168  GATC                 ^GATC
SfcI          142  CTGCAG               C^T[GA][CT]AG
SmlI          686  CTCAAG               C^T[CT][GA]AG
StyI          558  CCAAGG               C^C[AT][AT]GG
XbaI          435  TCTAGA               T^CTAGA
XcmI          101  CCAAGACAGCGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AvaII          66  GGACC                G^G[AT]CC
              478  GGTCC               
BanI          114  GGCGCC               G^G[CT][GA]CC
              847  GGCGCC              
BbeI          114  GGCGCC               GGCGC^C
              847  GGCGCC              
BpmI          338  CTGGAG               CTGGAG(16/14)
              363  CTGGAG              
BsaJI         176  CCGAGG               C^C[ACGT][ACGT]GG
              558  CCAAGG              
BsiYI         119  CCATCGAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              176  CCGAGGCGCGG         
BslI          119  CCATCGAGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              176  CCGAGGCGCGG         
BssKI         207  CCTGG                ^CC[ACGT]GG
              384  CCTGG               
BstF5I         53  CATCC                GGATG(2/0)
              413  CATCC               
BstNI         207  CCTGG                CC^[AT]GG
              384  CCTGG               
Cfr13I         66  GGACC                G^G[ACGT]CC
              478  GGTCC               
DdeI          482  CTGAG                C^T[ACGT]AG
              792  CTTAG               
DraII          65  AGGACCT              [GA]G^G[ACGT]CC[CT]
              477  AGGTCCT             
EcoO109I       65  AGGACCT              [GA]G^G[ACGT]CC[CT]
              477  AGGTCCT             
EcoRII        207  CCTGG                ^CC[AT]GG
              384  CCTGG               
FokI           53  CATCC                GGATG(9/13)
              413  CATCC               
HaeII         114  GGCGCC               [GA]GCGC^[CT]
              847  GGCGCC              
HgaI           46  GACGC                GACGC(5/10)
              154  GCGTC               
KasI          114  GGCGCC               G^GCGCC
              847  GGCGCC              
MaeIII        509  GTGAC                ^GT[ACGT]AC
              694  GTGAC               
MslI          413  CATCCTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              585  CATCATTGTG          
MspA1I         92  CAGCTG               C[AC]G^C[GT]G
              107  CAGCGG              
MvaI          207  CCTGG                CC^[AT]GG
              384  CCTGG               
NarI          114  GGCGCC               GG^CGCC
              847  GGCGCC              
PpuMI          65  AGGACCT              [GA]G^G[AT]CC[CT]
              477  AGGTCCT             
Sau96I         66  GGACC                G^G[ACGT]CC
              478  GGTCC               
ScrFI         207  CCTGG                CC^[ACGT]GG
              384  CCTGG               
SfoI          114  GGCGCC               GGC^GCC
              847  GGCGCC              
TfiI          216  GATTC                G^A[AT]TC
              827  GAATC               
Tsp45I        509  GTGAC                ^GT[GC]AC
              694  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Asp700I     BbvI        BsaHI       BstUI       Cac8I       
CfoI        Fnu4HI      HhaI        Hin1I       HinP1I      
HinfI       HphI        ItaI        MboII       MnlI        
MseI        MvnI        MwoI        NlaIII      NlaIV       
RsaI        SfaNI       TaqI        Tru9I       TseI        
Tsp509I     TthHB8I     XmnI        
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