Restriction map for pF1KE0150
 (Linear) (Six-base) MAPSORT of: KIEE0150.seq  Check: 938  from: 1  to: 945



KIEE0150.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     135     945 
   Size:        135     810
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     222     945 
   Size:        222     723
 
ApoI r'AATT_y

Cuts at:      0     916     945 
   Size:        916      29
 
AvaI C'yCGr_G

Cuts at:      0     175     945 
   Size:        175     770
 
BanI G'GyrC_C

Cuts at:      0     114     847     945 
   Size:        114     733      98

  Fragments arranged by size: 

                 733     114      98
 
BbeI G_GCGC'C

Cuts at:      0     118     851     945 
   Size:        118     733      94

  Fragments arranged by size: 

                 733     118      94
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     747     945 
   Size:        747     198
 
BfrBI ATG'CAT

Cuts at:      0       3     945 
   Size:          3     942
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     359     384     945 
   Size:        359      25     561

  Fragments arranged by size: 

                 561     359      25
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     671     945 
   Size:        671     274
 
BsaHI Gr'CG_yC

Cuts at:      0      47     115     154     848     945 
   Size:         47      68      39     694      97

  Fragments arranged by size: 

                 694      97      68      47      39
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     499     945 
   Size:        499     446
 
BseYI C'CCAG_C

Cuts at:      0     403     945 
   Size:        403     542
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     945 
   Size:        169     776
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     135     945 
   Size:        135     810
 
BsrBI CCG'CTC

Cuts at:      0     126     945 
   Size:        126     819
 
BsrDI GCAATG_nn'

Cuts at:      0     680     945 
   Size:        680     265
 
BsrFI r'CCGG_y

Cuts at:      0      76     945 
   Size:         76     869
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     565     945 
   Size:        565     380
 
Bsu36I CC'TnA_GG

Cuts at:      0     482     945 
   Size:        482     463
 
EaeI y'GGCC_r

Cuts at:      0      74     945 
   Size:         74     871
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      95     945 
   Size:         95     850
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     222     359     384     945 
   Size:        222     137      25     561

  Fragments arranged by size: 

                 561     222     137      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0      66     478     945 
   Size:         66     412     467

  Fragments arranged by size: 

                 467     412      66
 
EcoRI G'AATT_C

Cuts at:      0     916     945 
   Size:        916      29
 
HaeII r_GCGC'y

Cuts at:      0     118     851     945 
   Size:        118     733      94

  Fragments arranged by size: 

                 733     118      94
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      37      69     446     478     945 
   Size:         37      32     377      32     467

  Fragments arranged by size: 

                 467     377      37      32      32
 
KasI G'GCGC_C

Cuts at:      0     114     847     945 
   Size:        114     733      98

  Fragments arranged by size: 

                 733     114      98
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     945 
   Size:        307     638
 
MslI CAynn'nnrTG

Cuts at:      0     417     589     945 
   Size:        417     172     356

  Fragments arranged by size: 

                 417     356     172
 
MspA1I CmG'CkG

Cuts at:      0      94     109     945 
   Size:         94      15     836

  Fragments arranged by size: 

                 836      94      15
 
NaeI GCC'GGC

Cuts at:      0      78     945 
   Size:         78     867
 
NarI GG'CG_CC

Cuts at:      0     115     848     945 
   Size:        115     733      97

  Fragments arranged by size: 

                 733     115      97
 
NgoMIV G'CCGG_C

Cuts at:      0      76     945 
   Size:         76     869
 
NruI TCG'CGA

Cuts at:      0     224     945 
   Size:        224     721
 
NsiI A_TGCA'T

Cuts at:      0       5     945 
   Size:          5     940
 
NspI r_CATG'y

Cuts at:      0     704     945 
   Size:        704     241
 
PpuMI rG'GwC_Cy

Cuts at:      0      66     478     945 
   Size:         66     412     467

  Fragments arranged by size: 

                 467     412      66
 
PstI C_TGCA'G

Cuts at:      0     146     945 
   Size:        146     799
 
PvuII CAG'CTG

Cuts at:      0      94     945 
   Size:         94     851
 
SfcI C'TryA_G

Cuts at:      0     142     945 
   Size:        142     803
 
SfoI GGC'GCC

Cuts at:      0     116     849     945 
   Size:        116     733      96

  Fragments arranged by size: 

                 733     116      96
 
SmlI C'TyrA_G

Cuts at:      0     686     945 
   Size:        686     259
 
StyI C'CwwG_G

Cuts at:      0     558     945 
   Size:        558     387
 
TatI w'GTAC_w

Cuts at:      0     576     945 
   Size:        576     369
 
XbaI T'CTAG_A

Cuts at:      0     435     945 
   Size:        435     510
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     108     945 
   Size:        108     837
 
XmnI GAAnn'nnTTC

Cuts at:      0     714     831     920     945 
   Size:        714     117      89      25

  Fragments arranged by size: 

                 714     117      89      25
 

 Enzymes that do cut:

     AarI     AcuI     ApoI     AvaI     BanI     BbeI     BbsI    BfrBI
     BpmI    BpuEI    BsaHI    BseRI    BseYI     BsgI    BspMI    BsrBI
    BsrDI    BsrFI    BstXI   Bsu36I     EaeI     EciI  Eco57MI EcoO109I
    EcoRI    HaeII    Hin4I     KasI     MmeI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NruI     NsiI     NspI    PpuMI     PstI    PvuII
     SfcI     SfoI     SmlI     StyI     TatI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MscI
     NcoI     NdeI     NheI     NotI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XhoI     XmaI     ZraI
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