Restriction map for pF1KE0101
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaII       AviII       
AvrII       BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BlnI        
BmyI        BpmI        BpuAI       BsaAI       BsaHI       
BsaI        BseAI       BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmI        Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRI       
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
ItaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MboII       MfeI        MluI        
MroI        MspA1I      MunI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TseI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AgeI           38  ACCGGT               A^CCGGT
AlwI          252  GATCC                GGATC(4/5)
AlwNI         395  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          388  ATTAAT               AT^TAAT
AsnI          388  ATTAAT               AT^TAAT
AvaI          336  CTCGGG               C^[CT]CG[GA]G
BalI          287  TGGCCA               TGG^CCA
BanI           78  GGTGCC               G^G[CT][GA]CC
BglI          403  GCCACTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          21  AGATCT               A^GATCT
BlpI          293  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      293  GCTTAGC              GC^T[ACGT]AGC
BsaWI          38  ACCGGT               [AT]^CCGG[AT]
BseRI         179  GAGGAG               GAGGAG(10/8)
BsmAI         182  GAGAC                GTCTC(1/5)
BsmFI         339  GGGAC                GGGAC(10/14)
BsoBI         336  CTCGGG               C^[CT]CG[GA]G
BsrFI          38  ACCGGT               [GA]^CCGG[CT]
BsrI          406  ACTGG                ACTGG(1/-1)
BstAPI        412  GCATATAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        480  GGTCACC              G^GT[ACGT]ACC
BstF5I         30  CATCC                GGATG(2/0)
BstPI         480  GGTCACC              G^GT[ACGT]ACC
BstYI          21  AGATCT               [GA]^GATC[CT]
CelII         293  GCTTAGC              GC^T[ACGT]AGC
Cfr10I         38  ACCGGT               [GA]^CCGG[CT]
EaeI          287  TGGCCA               [CT]^GGCC[GA]
EcoO65I       480  GGTCACC              G^GT[ACGT]ACC
EcoRV         460  GATATC               GAT^ATC
FokI           30  CATCC                GGATG(9/13)
HaeIII        288  GGCC                 GG^CC
HapII          39  CCGG                 C^CGG
HpaII          39  CCGG                 C^CGG
MflI           21  AGATCT               [GA]^GATC[CT]
MluNI         287  TGGCCA               TGG^CCA
MscI          287  TGGCCA               TGG^CCA
MseI          389  TTAA                 T^TAA
MspI           39  CCGG                 C^CGG
NlaIV          78  GGTGCC               GG[ACGT]^[ACGT]CC
PinAI          38  ACCGGT               A^CCGGT
PleI          150  GACTC                GAGTC(4/5)
PshBI         388  ATTAAT               AT^TAAT
SfcI          473  CTACAG               C^T[GA][CT]AG
SgrAI          37  CACCGGTG             C[GA]^CCGG[CT]G
SmlI          145  CTCAAG               C^T[CT][GA]AG
Tru9I         389  TTAA                 T^TAA
XhoII          21  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

BsaBI         190  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              456  GATGGATATC          
BsgI           25  CTGCAC               GTGCAG(16/14)
              105  CTGCAC              
Cac8I          54  GCCAGC               GC[ACGT]^[ACGT]GC
              289  GCCAGC              
DdeI          294  CTTAG                C^T[ACGT]AG
              356  CTGAG               
DpnII          22  GATC                 ^GATC
              252  GATC                
DsaI          255  CCATGG               C^C[GA][CT]GG
              274  CCATGG              
HphI          160  GGTGA                GGTGA(8/7)
              482  TCACC               
MaeIII        223  GTGAC                ^GT[ACGT]AC
              481  GTCAC               
MamI          190  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              456  GATGGATATC          
MboI           22  GATC                 ^GATC
              252  GATC                
MslI           35  CACACCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              483  CACCTATGTG          
NcoI          255  CCATGG               C^CATGG
              274  CCATGG              
NdeII          22  GATC                 ^GATC
              252  GATC                
Sau3AI         22  GATC                 ^GATC
              252  GATC                
TaqI          188  TCGA                 T^CGA
              530  TCGA                
TfiI          195  GAATC                G^A[AT]TC
              527  GAATC               
Tsp45I        223  GTGAC                ^GT[GC]AC
              481  GTCAC               
TspRI         405  CACTG                CA[GC]TG[ACGT][ACGT]^
              476  CAGTG               
TthHB8I       188  TCGA                 T^CGA
              530  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AcsI        AluI        ApoI        BsaJI       BssKI       
BstNI       EcoRII      EcoT14I     HinfI       MnlI        
MvaI        MwoI        NlaIII      ScrFI       StyI        
Tsp509I     
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