Restriction map for pF1KE0410
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BamHI       BbeI        
BbvCI       BcgI        BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BsaBI       
BsaHI       BsaI        BseAI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoT14I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeIII      MamI        MfeI        
MflI        MluI        MroI        MseI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmeI        PshAI       PshBI       
Psp1406I    RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StyI        SwaI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AfaI          243  GTAC                 GT^AC
AlwI          351  GATCC                GGATC(4/5)
BalI          201  TGGCCA               TGG^CCA
BanII         184  GAGCCC               G[GA]GC[CT]^C
BbrPI         175  CACGTG               CAC^GTG
BciVI         142  GTATCC               GTATCC(6/5)
BlpI          549  GCTCAGC              GC^T[ACGT]AGC
BpmI          466  CTGGAG               CTGGAG(16/14)
Bpu1102I      549  GCTCAGC              GC^T[ACGT]AGC
BsaAI         175  CACGTG               [CT]AC^GT[GA]
BsaWI          80  TCCGGT               [AT]^CCGG[AT]
BsiEI         534  CGATCG               CG[GA][CT]^CG
BsmFI         444  GGGAC                GGGAC(10/14)
BsrI          193  CCAGT                ACTGG(1/-1)
CelII         549  GCTCAGC              GC^T[ACGT]AGC
CfoI          252  GCGC                 GCG^C
DraII         444  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII        175  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          296  CCGTGG               C^C[GA][CT]GG
EaeI          201  TGGCCA               [CT]^GGCC[GA]
EcoO109I      444  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRV          76  GATATC               GAT^ATC
HhaI          252  GCGC                 GCG^C
HinP1I        252  GCGC                 G^CGC
MluNI         201  TGGCCA               TGG^CCA
MscI          201  TGGCCA               TGG^CCA
MslI          189  CATGCCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PmaCI         175  CACGTG               CAC^GTG
PmlI          175  CACGTG               CAC^GTG
PpuMI         444  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          502  CTGCAG               CTGCA^G
PvuI          534  CGATCG               CGAT^CG
RsaI          243  GTAC                 GT^AC
SfcI          502  CTGCAG               C^T[GA][CT]AG
StuI          215  AGGCCT               AGG^CCT
TaqI          533  TCGA                 T^CGA
TthHB8I       533  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AciI          492  GCGG                 CCGC(-3/-1)
              519  GCGG                
AvaI          439  CTCGGG               C^[CT]CG[GA]G
              449  CCCGAG              
AvaII         342  GGACC                G^G[AT]CC
              445  GGACC               
BbsI          522  GAAGAC               GAAGAC(2/6)
              529  GTCTTC              
BpuAI         522  GAAGAC               GAAGAC(2/6)
              529  GTCTTC              
BsaJI         296  CCGTGG               C^C[ACGT][ACGT]GG
              438  CCTCGG              
BsoBI         439  CTCGGG               C^[CT]CG[GA]G
              449  CCCGAG              
BspMI         239  GCAGGT               ACCTGC(4/8)
              504  GCAGGT              
BssKI         199  CCTGG                ^CC[ACGT]GG
              465  CCTGG               
BstNI         199  CCTGG                CC^[AT]GG
              465  CCTGG               
Cfr13I        342  GGACC                G^G[ACGT]CC
              445  GGACC               
DdeI          374  CTGAG                C^T[ACGT]AG
              550  CTCAG               
DpnII         351  GATC                 ^GATC
              535  GATC                
EcoRII        199  CCTGG                ^CC[AT]GG
              465  CCTGG               
EcoT22I        90  ATGCAT               ATGCA^T
              365  ATGCAT              
HapII          81  CCGG                 C^CGG
              361  CCGG                
HpaII          81  CCGG                 C^CGG
              361  CCGG                
MaeII         176  ACGT                 A^CGT
              209  ACGT                
MboI          351  GATC                 ^GATC
              535  GATC                
MspI           81  CCGG                 C^CGG
              361  CCGG                
MvaI          199  CCTGG                CC^[AT]GG
              465  CCTGG               
NdeII         351  GATC                 ^GATC
              535  GATC                
NsiI           90  ATGCAT               ATGCA^T
              365  ATGCAT              
PflMI         193  CCAGTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              204  CCATGACGTGG         
PvuII          50  CAGCTG               CAG^CTG
              425  CAGCTG              
Sau3AI        351  GATC                 ^GATC
              535  GATC                
Sau96I        342  GGACC                G^G[ACGT]CC
              445  GGACC               
ScrFI         199  CCTGG                CC^[ACGT]GG
              465  CCTGG               
Van91I        193  CCAGTGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              204  CCATGACGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AluI        AlwNI       BanI        BbvI        BmyI        
BseRI       BsgI        BsiYI       BslI        Bsp1286I    
BstAPI      BstF5I      Cac8I       Fnu4HI      FokI        
HaeIII      ItaI        MboII       MnlI        MspA1I      
MwoI        NlaIII      NlaIV       SfaNI       TseI        
TspRI       
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