Restriction map for pF1KB4567
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BcgI        BciVI       BclI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstXI       BstZ17I     
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      FauI        FbaI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HindIII     HpaI        KasI        KpnI        
KspI        MaeII       MfeI        MluI        MroI        
MslI        MunI        MvnI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        Tth111I     XhoI        XmaCI       XmaI        
XmnI        


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# ONE CUT ENZYMES (   39 enzymes) #
###################################

AflII         589  CTTAAG               C^TTAAG
AlwNI         932  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          638  GGGCCC               GGGCC^C
AspHI        2218  GAGCTC               G[AT]GC[AT]^C
BanI         1152  GGCACC               G^G[CT][GA]CC
BbvCI        1584  GCTGAGG              CCTCAGC(-5/-2)
BcnI          636  CCGGG                CC^[GC]GG
BfrI          589  CTTAAG               C^TTAAG
BglI         1662  GCCCAAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI         2051  GTGCAG               GTGCAG(16/14)
BsiHKAI      2218  GAGCTC               G[AT]GC[AT]^C
BsmBI        1207  CGTCTC               CGTCTC(1/5)
BstEII        392  GGTCACC              G^GT[ACGT]ACC
BstPI         392  GGTCACC              G^GT[ACGT]ACC
DraI         1339  TTTAAA               TTT^AAA
EcoNI         408  CCTCGTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       392  GGTCACC              G^GT[ACGT]ACC
EcoRI        2155  GAATTC               G^AATTC
EcoRV         422  GATATC               GAT^ATC
EcoT22I      1289  ATGCAT               ATGCA^T
HapII         636  CCGG                 C^CGG
HgaI         1206  GCGTC                GACGC(5/10)
HincII         76  GTCAAC               GT[CT]^[GA]AC
HindII         76  GTCAAC               GT[CT]^[GA]AC
HpaII         636  CCGG                 C^CGG
MspI          636  CCGG                 C^CGG
NciI          636  CCGGG                CC^[GC]GG
NdeI         1286  CATATG               CA^TATG
NsiI         1289  ATGCAT               ATGCA^T
NspI         1853  GCATGT               [GA]CATG^[CT]
PflMI        2181  CCAGGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         386  GACTCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         2218  GAGCTC               GAGCT^C
SfcI         1778  CTGTAG               C^T[GA][CT]AG
SmlI          589  CTTAAG               C^T[CT][GA]AG
SspI          108  AATATT               AAT^ATT
StuI         1535  AGGCCT               AGG^CCT
Van91I       2181  CCAGGATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          330  CCACGCTGCTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI          758  GTAGAC               GT^[AC][GT]AC
             2134  GTAGAC              
AciI          577  CCGC                 CCGC(-3/-1)
             1767  CCGC                
AcsI          896  AAATTC               [GA]^AATT[CT]
             2155  GAATTC              
AfaI          120  GTAC                 GT^AC
              819  GTAC                
AocI          937  CCTGAGG              CC^T[ACGT]AGG
             1705  CCTGAGG             
ApoI          896  AAATTC               [GA]^AATT[CT]
             2155  GAATTC              
BsaBI        1711  GATGTCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1717  GATCTAGATC          
BseRI        1124  CTCCTC               GAGGAG(10/8)
             1539  CTCCTC              
BsmFI         199  GGGAC                GGGAC(10/14)
             1827  GTCCC               
BsrDI          27  GCAATG               GCAATG(2/0)
             1135  GCAATG              
BsrI          878  CCAGT                ACTGG(1/-1)
             1106  CCAGT               
Bsu36I        937  CCTGAGG              CC^T[ACGT]AGG
             1705  CCTGAGG             
DraII         199  GGGACCC              [GA]G^G[ACGT]CC[CT]
              933  AGGTCCT             
Eco81I        937  CCTGAGG              CC^T[ACGT]AGG
             1705  CCTGAGG             
EcoO109I      199  GGGACCC              [GA]G^G[ACGT]CC[CT]
              933  AGGTCCT             
MamI         1711  GATGTCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1717  GATCTAGATC          
PpuMI         199  GGGACCC              [GA]G^G[AT]CC[CT]
              933  AGGTCCT             
RsaI          120  GTAC                 GT^AC
              819  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AluI        AlwI        AvaII       BalI        BanII       
BbsI        BbvI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsiYI       
BslI        BsmAI       Bsp1286I    BspMI       BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EaeI        EarI        EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PvuII       
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XbaI        XhoII       
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