Restriction map for pF1KB4567
 (Linear) (Six-base) MAPSORT of: KIBB4567.seq  Check: 6420  from: 1  to: 2280



KIBB4567.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1164    1215    2280 
   Size:       1164      51    1065

  Fragments arranged by size: 

                1164    1065      51
 
AccI GT'mk_AC

Cuts at:      0     759    2135    2280 
   Size:        759    1376     145

  Fragments arranged by size: 

                1376     759     145
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     338     422    1697    1747    1774    2171    2280 
   Size:        155     183      84    1275      50      27     397     109

  Fragments arranged by size: 

                1275     397     183     155     109      84      50      27
            
 
AflII C'TTAA_G

Cuts at:      0     589    2280 
   Size:        589    1691
 
AlwNI CAG_nnn'CTG

Cuts at:      0     937    2280 
   Size:        937    1343
 
ApaI G_GGCC'C

Cuts at:      0     642    2280 
   Size:        642    1638
 
ApoI r'AATT_y

Cuts at:      0     896    2155    2280 
   Size:        896    1259     125

  Fragments arranged by size: 

                1259     896     125
 
BanI G'GyrC_C

Cuts at:      0    1152    2280 
   Size:       1152    1128
 
BanII G_rGCy'C

Cuts at:      0     642    1816    2222    2280 
   Size:        642    1174     406      58

  Fragments arranged by size: 

                1174     642     406      58
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1192    1565    1685    2280 
   Size:       1192     373     120     595

  Fragments arranged by size: 

                1192     595     373     120
 
BbvCI CC'TCA_GC

Cuts at:      0    1585    2280 
   Size:       1585     695
 
BfrBI ATG'CAT

Cuts at:      0    1291    2280 
   Size:       1291     989
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1668    2280 
   Size:       1668     612
 
BglII A'GATC_T

Cuts at:      0     379     668    1722    2280 
   Size:        379     289    1054     558

  Fragments arranged by size: 

                1054     558     379     289
 
Bme1580I G_kGCm'C

Cuts at:      0     642     929    1376    2280 
   Size:        642     287     447     904

  Fragments arranged by size: 

                 904     642     447     287
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     362    1089    1869    2280 
   Size:        362     727     780     411

  Fragments arranged by size: 

                 780     727     411     362
 
Bpu10I CC'TnA_GC

Cuts at:      0    1499    1585    2280 
   Size:       1499      86     695

  Fragments arranged by size: 

                1499     695      86
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1159    1425    1453    2280 
   Size:       1159     266      28     827

  Fragments arranged by size: 

                1159     827     266      28
 
BsaBI GATnn'nnATC

Cuts at:      0    1715    1721    2280 
   Size:       1715       6     559

  Fragments arranged by size: 

                1715     559       6
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2195    2225    2280 
   Size:       2195      30      55

  Fragments arranged by size: 

                2195      55      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1115    1530    2280 
   Size:       1115     415     750

  Fragments arranged by size: 

                1115     750     415
 
BseYI C'CCAG_C

Cuts at:      0     182     240    1439    2280 
   Size:        182      58    1199     841

  Fragments arranged by size: 

                1199     841     182      58
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2072    2280 
   Size:       2072     208
 
BsiHKAI G_wGCw'C

Cuts at:      0    2222    2280 
   Size:       2222      58
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1213    2280 
   Size:       1213    1067
 
Bsp1286I G_dGCh'C

Cuts at:      0     642     929    1376    1816    2222    2280 
   Size:        642     287     447     440     406      58

  Fragments arranged by size: 

                 642     447     440     406     287      58
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1164    1177    1215    2280 
   Size:       1164      13      38    1065

  Fragments arranged by size: 

                1164    1065      38      13
 
BsrDI GCAATG_nn'

Cuts at:      0      34    1142    2280 
   Size:         34    1108    1138

  Fragments arranged by size: 

                1138    1108      34
 
BstEII G'GTnAC_C

Cuts at:      0     392    2280 
   Size:        392    1888
 
BstYI r'GATC_y

Cuts at:      0     379     447     668    1389    1722    2280 
   Size:        379      68     221     721     333     558

  Fragments arranged by size: 

                 721     558     379     333     221      68
 
Bsu36I CC'TnA_GG

Cuts at:      0     938    1706    2280 
   Size:        938     768     574

  Fragments arranged by size: 

                 938     768     574
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1596    1631    2280 
   Size:       1596      35     649

  Fragments arranged by size: 

                1596     649      35
 
DraI TTT'AAA

Cuts at:      0    1341    2280 
   Size:       1341     939
 
EaeI y'GGCC_r

Cuts at:      0     812    1214    1597    2280 
   Size:        812     402     383     683

  Fragments arranged by size: 

                 812     683     402     383
 
EarI CTCTTCn'nnn_

Cuts at:      0     151    1412    1865    1881    1987    2270    2280 
   Size:        151    1261     453      16     106     283      10

  Fragments arranged by size: 

                1261     453     283     151     106      16      10
 
EcoICRI GAG'CTC

Cuts at:      0    2220    2280 
   Size:       2220      60
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     338     362     422    1089    1697    1747    1774 
   Size:        155     183      24      60     667     608      50      27

Cuts at:   1774    1869    2171    2280 
   Size:         95     302     109

  Fragments arranged by size: 

                 667     608     302     183     155     109      95      60
                  50      27      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     412    2280 
   Size:        412    1868
 
EcoO109I rG'GnC_Cy

Cuts at:      0     200     934    2280 
   Size:        200     734    1346

  Fragments arranged by size: 

                1346     734     200
 
EcoRI G'AATT_C

Cuts at:      0    2155    2280 
   Size:       2155     125
 
EcoRV GAT'ATC

Cuts at:      0     424    2280 
   Size:        424    1856
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1341    1760    2280 
   Size:       1341     419     520

  Fragments arranged by size: 

                1341     520     419
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     371     403    1179    1211    2195    2227    2280 
   Size:        371      32     776      32     984      32      53

  Fragments arranged by size: 

                 984     776     371      53      32      32      32
 
HincII GTy'rAC

Cuts at:      0      78    2280 
   Size:         78    2202
 
MscI TGG'CCA

Cuts at:      0     814    1216    1599    2280 
   Size:        814     402     383     681

  Fragments arranged by size: 

                 814     681     402     383
 
MspA1I CmG'CkG

Cuts at:      0     133     579     829    1241    1570    2280 
   Size:        133     446     250     412     329     710

  Fragments arranged by size: 

                 710     446     412     329     250     133
 
NdeI CA'TA_TG

Cuts at:      0    1287    2280 
   Size:       1287     993
 
NsiI A_TGCA'T

Cuts at:      0    1293    2280 
   Size:       1293     987
 
NspI r_CATG'y

Cuts at:      0    1857    2280 
   Size:       1857     423
 
PasI CC'CwG_GG

Cuts at:      0     235    1474    2280 
   Size:        235    1239     806

  Fragments arranged by size: 

                1239     806     235
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2187    2280 
   Size:       2187      93
 
PpuMI rG'GwC_Cy

Cuts at:      0     200     934    2280 
   Size:        200     734    1346

  Fragments arranged by size: 

                1346     734     200
 
PshAI GACnn'nnGTC

Cuts at:      0     390    2280 
   Size:        390    1890
 
PspOMI G'GGCC_C

Cuts at:      0     638    2280 
   Size:        638    1642
 
PvuII CAG'CTG

Cuts at:      0     133     829    1241    1570    2280 
   Size:        133     696     412     329     710

  Fragments arranged by size: 

                 710     696     412     329     133
 
SacI G_AGCT'C

Cuts at:      0    2222    2280 
   Size:       2222      58
 
SanDI GG'GwC_CC

Cuts at:      0     200    2280 
   Size:        200    2080
 
SfcI C'TryA_G

Cuts at:      0    1778    2280 
   Size:       1778     502
 
SmlI C'TyrA_G

Cuts at:      0     589    2280 
   Size:        589    1691
 
SspI AAT'ATT

Cuts at:      0     110    2280 
   Size:        110    2170
 
StuI AGG'CCT

Cuts at:      0    1537    2280 
   Size:       1537     743
 
StyI C'CwwG_G

Cuts at:      0    1532    1634    1919    2280 
   Size:       1532     102     285     361

  Fragments arranged by size: 

                1532     361     285     102
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     967    1495    1870    2280 
   Size:        967     528     375     410

  Fragments arranged by size: 

                 967     528     410     375
 
XbaI T'CTAG_A

Cuts at:      0     382     690    1719    2280 
   Size:        382     308    1029     561

  Fragments arranged by size: 

                1029     561     382     308
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     337    2280 
   Size:        337    1943
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII    AlwNI     ApaI     ApoI     BanI
    BanII     BbsI    BbvCI    BfrBI     BglI    BglII Bme1580I     BpmI
   Bpu10I     BsaI    BsaBI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspMI    BsrDI   BstEII    BstYI   Bsu36I    CspCI
     DraI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    Hin4I   HincII     MscI   MspA1I     NdeI     NsiI
     NspI     PasI    PflMI    PpuMI    PshAI   PspOMI    PvuII     SacI
    SanDI     SfcI     SmlI     SspI     StuI     StyI    TaqII     XbaI
     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI     BcgI    BciVI     BclI     BlpI    BmgBI
     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaHI    BsaWI    BsiEI
    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI   DraIII     DrdI     EagI     EciI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI     PsiI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SwaI     TatI     TstI  Tth111I     XhoI
     XmaI     XmnI     ZraI
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