Restriction map for pF1KB8824
 (Linear) (Six-base) MAPSORT of: KIBB8824.seq  Check: 6481  from: 1  to: 1242



KIBB8824.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     961    1242 
   Size:        294     667     281

  Fragments arranged by size: 

                 667     294     281
 
AflIII A'CryG_T

Cuts at:      0     282    1242 
   Size:        282     960
 
AleI CACnn'nnGTG

Cuts at:      0      19     232    1242 
   Size:         19     213    1010

  Fragments arranged by size: 

                1010     213      19
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     313    1242 
   Size:        313     929
 
AlwNI CAG_nnn'CTG

Cuts at:      0     843     939    1117    1242 
   Size:        843      96     178     125

  Fragments arranged by size: 

                 843     178     125      96
 
ApaI G_GGCC'C

Cuts at:      0     879    1242 
   Size:        879     363
 
AvrII C'CTAG_G

Cuts at:      0     213    1242 
   Size:        213    1029
 
BanI G'GyrC_C

Cuts at:      0     180     379     494     807     816    1242 
   Size:        180     199     115     313       9     426

  Fragments arranged by size: 

                 426     313     199     180     115       9
 
BanII G_rGCy'C

Cuts at:      0     348     473     595     879    1142    1242 
   Size:        348     125     122     284     263     100

  Fragments arranged by size: 

                 348     284     263     125     122     100
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     498     742    1242 
   Size:        498     244     500

  Fragments arranged by size: 

                 500     498     244
 
BglI GCCn_nnn'nGGC

Cuts at:      0     133    1242 
   Size:        133    1109
 
Bme1580I G_kGCm'C

Cuts at:      0     497     629     812     821     879    1242 
   Size:        497     132     183       9      58     363

  Fragments arranged by size: 

                 497     363     183     132      58       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     853    1242 
   Size:        853     389
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1143    1242 
   Size:       1143      99
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31    1059    1242 
   Size:         31    1028     183

  Fragments arranged by size: 

                1028     183      31
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     633    1242 
   Size:        601      32     609

  Fragments arranged by size: 

                 609     601      32
 
BsaAI yAC'GTr

Cuts at:      0     283    1242 
   Size:        283     959
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1032    1062    1242 
   Size:       1032      30     180

  Fragments arranged by size: 

                1032     180      30
 
BseYI C'CCAG_C

Cuts at:      0    1007    1071    1098    1228    1242 
   Size:       1007      64      27     130      14

  Fragments arranged by size: 

                1007     130      64      27      14
 
BsiHKAI G_wGCw'C

Cuts at:      0      26    1142    1242 
   Size:         26    1116     100

  Fragments arranged by size: 

                1116     100      26
 
Bsp1286I G_dGCh'C

Cuts at:      0      26     348     473     497     595     629     812     821 
   Size:         26     322     125      24      98      34     183       9

Cuts at:    821     879    1142    1242 
   Size:         58     263     100

  Fragments arranged by size: 

                 322     263     183     125     100      98      58      34
                  26      24       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     593    1166    1242 
   Size:        593     573      76

  Fragments arranged by size: 

                 593     573      76
 
BsrFI r'CCGG_y

Cuts at:      0     797    1242 
   Size:        797     445
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1050    1242 
   Size:       1050     192
 
BstYI r'GATC_y

Cuts at:      0    1113    1242 
   Size:       1113     129
 
Bsu36I CC'TnA_GG

Cuts at:      0     698    1242 
   Size:        698     544
 
BtgI C'CryG_G

Cuts at:      0     849     978    1242 
   Size:        849     129     264

  Fragments arranged by size: 

                 849     264     129
 
BtsI GCAGTG_nn'

Cuts at:      0     162     568     899    1242 
   Size:        162     406     331     343

  Fragments arranged by size: 

                 406     343     331     162
 
EaeI y'GGCC_r

Cuts at:      0     134     443     764     880    1242 
   Size:        134     309     321     116     362

  Fragments arranged by size: 

                 362     321     309     134     116
 
EarI CTCTTCn'nnn_

Cuts at:      0     170    1242 
   Size:        170    1072
 
EcoICRI GAG'CTC

Cuts at:      0    1140    1242 
   Size:       1140     102
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     294     961    1059    1242 
   Size:         31     263     667      98     183

  Fragments arranged by size: 

                 667     263     183      98      31
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89     875    1242 
   Size:         89     786     367

  Fragments arranged by size: 

                 786     367      89
 
HaeII r_GCGC'y

Cuts at:      0    1095    1242 
   Size:       1095     147
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     622     654     845     877    1242 
   Size:        622      32     191      32     365

  Fragments arranged by size: 

                 622     365     191      32      32
 
HincII GTy'rAC

Cuts at:      0     951    1242 
   Size:        951     291
 
MscI TGG'CCA

Cuts at:      0     445     766    1242 
   Size:        445     321     476

  Fragments arranged by size: 

                 476     445     321
 
MslI CAynn'nnrTG

Cuts at:      0      19     232     319     500     964    1242 
   Size:         19     213      87     181     464     278

  Fragments arranged by size: 

                 464     278     213     181      87      19
 
MspA1I CmG'CkG

Cuts at:      0     187     542    1011    1242 
   Size:        187     355     469     231

  Fragments arranged by size: 

                 469     355     231     187
 
NaeI GCC'GGC

Cuts at:      0     799    1242 
   Size:        799     443
 
NcoI C'CATG_G

Cuts at:      0     849    1242 
   Size:        849     393
 
NgoMIV G'CCGG_C

Cuts at:      0     797    1242 
   Size:        797     445
 
NspI r_CATG'y

Cuts at:      0     300     324     892    1242 
   Size:        300      24     568     350

  Fragments arranged by size: 

                 568     350     300      24
 
PasI CC'CwG_GG

Cuts at:      0     803    1242 
   Size:        803     439
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     518    1242 
   Size:        518     724
 
PfoI T'CCnGG_A

Cuts at:      0     452     749    1242 
   Size:        452     297     493

  Fragments arranged by size: 

                 493     452     297
 
PpuMI rG'GwC_Cy

Cuts at:      0      89    1242 
   Size:         89    1153
 
PspOMI G'GGCC_C

Cuts at:      0     875    1242 
   Size:        875     367
 
PstI C_TGCA'G

Cuts at:      0     590    1242 
   Size:        590     652
 
PvuII CAG'CTG

Cuts at:      0     187    1011    1242 
   Size:        187     824     231

  Fragments arranged by size: 

                 824     231     187
 
SacI G_AGCT'C

Cuts at:      0    1142    1242 
   Size:       1142     100
 
SfcI C'TryA_G

Cuts at:      0     188     586    1242 
   Size:        188     398     656

  Fragments arranged by size: 

                 656     398     188
 
SmlI C'TyrA_G

Cuts at:      0     616     648    1242 
   Size:        616      32     594

  Fragments arranged by size: 

                 616     594      32
 
SphI G_CATG'C

Cuts at:      0     892    1242 
   Size:        892     350
 
StuI AGG'CCT

Cuts at:      0      52     365    1242 
   Size:         52     313     877

  Fragments arranged by size: 

                 877     313      52
 
StyI C'CwwG_G

Cuts at:      0     213     661     677     849    1242 
   Size:        213     448      16     172     393

  Fragments arranged by size: 

                 448     393     213     172      16
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     795    1021    1063    1242 
   Size:        795     226      42     179

  Fragments arranged by size: 

                 795     226     179      42
 
TatI w'GTAC_w

Cuts at:      0     368    1242 
   Size:        368     874
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     519     551    1032    1064    1242 
   Size:        519      32     481      32     178

  Fragments arranged by size: 

                 519     481     178      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     191    1242 
   Size:        191    1051
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI    AvrII     BanI
    BanII     BbsI     BglI Bme1580I     BmrI     BplI     BpmI    BpuEI
    BsaAI    BsaXI    BseYI  BsiHKAI Bsp1286I    BspMI    BsrFI   BstAPI
    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I    HaeII    Hin4I   HincII     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI
     PstI    PvuII     SacI     SfcI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI   Bpu10I     BsaI    BsaBI    BsaHI
    BsaWI    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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