Restriction map for pF1KB5207
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AccI        AccIII      AclI        AflII       AflIII      
AgeI        AhdI        AlwNI       AocI        ApaI        
AscI        AseI        AsnI        AspEI       AviII       
AvrII       BanII       BbrPI       BcgI        BciVI       
BfrI        BglII       BlnI        BsaAI       BsaBI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BspLU11I    
BsrFI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoT22I     FseI        FspI        
HindIII     HpaI        KspI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NgoMIV      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        SspI        
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   67 enzymes) #
###################################

AatII         214  GACGTC               GACGT^C
Acc65I       1443  GGTACC               G^GTACC
AccII        1157  CGCG                 CG^CG
Aor51HI       795  AGCGCT               AGC^GCT
Asp700I       971  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1443  GGTACC               G^GTACC
AspI         1356  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          839  CCCGGG               C^[CT]CG[GA]G
BalI         1004  TGGCCA               TGG^CCA
BbeI          169  GGCGCC               GGCGC^C
BclI          644  TGATCA               T^GATCA
BglI           37  GCCTCCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmI         1578  GCATTC               GAATGC(1/-1)
BsoBI         839  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1068  TGTACA               T^GTACA
BspHI         431  TCATGA               T^CATGA
BspMI        1486  GCAGGT               ACCTGC(4/8)
BsrBI         104  CCGCTC               CCGCTC(-3/-3)
BsrGI        1068  TGTACA               T^GTACA
BssSI         833  CTCGTG               CACGAG(-5/-1)
BstUI        1157  CGCG                 CG^CG
BstXI        1007  CCACTGACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII        824  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         1573  GAAGAG               CTCTTC(1/4)
Eco47III      795  AGCGCT               AGC^GCT
EcoRV        1306  GATATC               GAT^ATC
FauI           99  GCGGG                CCCGC(4/6)
FbaI          644  TGATCA               T^GATCA
HgaI           70  GCGTC                GACGC(5/10)
HincII       1353  GTTGAC               GT[CT]^[GA]AC
HindII       1353  GTTGAC               GT[CT]^[GA]AC
KasI          169  GGCGCC               G^GCGCC
KpnI         1443  GGTACC               GGTAC^C
Ksp632I      1573  GAAGAG               CTCTTC(1/4)
MluNI        1004  TGGCCA               TGG^CCA
MscI         1004  TGGCCA               TGG^CCA
MvnI         1157  CGCG                 CG^CG
NarI          169  GGCGCC               GG^CGCC
NcoI          678  CCATGG               C^CATGG
NdeI         1429  CATATG               CA^TATG
NheI          576  GCTAGC               G^CTAGC
PflFI        1356  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         300  CCAGCATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         111  GACGGCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         1282  CTGCAG               CTGCA^G
PvuII        1187  CAGCTG               CAG^CTG
RcaI          431  TCATGA               T^CATGA
SapI         1573  GAAGAGC              GCTCTTC(1/4)
SbfI         1281  CCTGCAGG             CCTGCA^GG
ScaI          372  AGTACT               AGT^ACT
SexAI         825  ACCTGGT              A^CC[AT]GGT
SfcI         1282  CTGCAG               C^T[GA][CT]AG
SfiI           36  GGCCTCCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          169  GGCGCC               GGC^GCC
SmaI          839  CCCGGG               CCC^GGG
Sse8387I     1281  CCTGCAGG             CCTGCA^GG
SspBI        1068  TGTACA               T^GTACA
StuI         1514  AGGCCT               AGG^CCT
TaqI          569  TCGA                 T^CGA
Tsp45I        550  GTGAC                ^GT[GC]AC
Tth111I      1356  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       569  TCGA                 T^CGA
Van91I        300  CCAGCATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1200  TCTAGA               T^CTAGA
XmaCI         839  CCCGGG               C^CCGGG
XmaI          839  CCCGGG               C^CCGGG
XmnI          971  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcsI          398  AAATTC               [GA]^AATT[CT]
              542  AAATTC              
AcyI          169  GGCGCC               G[GA]^CG[CT]C
              214  GACGTC              
Alw44I        386  GTGCAC               G^TGCAC
             1367  GTGCAC              
ApaLI         386  GTGCAC               G^TGCAC
             1367  GTGCAC              
ApoI          398  AAATTC               [GA]^AATT[CT]
              542  AAATTC              
BamHI        1194  GGATCC               G^GATCC
             1224  GGATCC              
BanI          169  GGCGCC               G^G[CT][GA]CC
             1443  GGTACC              
BbsI         1345  GAAGAC               GAAGAC(2/6)
             1417  GTCTTC              
BbvCI         502  GCTGAGG              CCTCAGC(-5/-2)
             1411  GCTGAGG             
BbvI           28  GCTGC                GCAGC(8/12)
               75  GCTGC               
BfaI          577  CTAG                 C^TAG
             1201  CTAG                
BlpI           85  GCTCAGC              GC^T[ACGT]AGC
             1168  GCTGAGC             
Bpu1102I       85  GCTCAGC              GC^T[ACGT]AGC
             1168  GCTGAGC             
BpuAI        1345  GAAGAC               GAAGAC(2/6)
             1417  GTCTTC              
BsaHI         169  GGCGCC               G[GA]^CG[CT]C
              214  GACGTC              
CelII          85  GCTCAGC              GC^T[ACGT]AGC
             1168  GCTGAGC             
DsaI          678  CCATGG               C^C[GA][CT]GG
             1263  CCGTGG              
EaeI           23  TGGCCG               [CT]^GGCC[GA]
             1004  TGGCCA              
EcoT14I       590  CCAAGG               C^C[AT][AT]GG
              678  CCATGG              
HaeII         169  GGCGCC               [GA]GCGC^[CT]
              795  AGCGCT              
Hin1I         169  GGCGCC               G[GA]^CG[CT]C
              214  GACGTC              
MaeI          577  CTAG                 C^TAG
             1201  CTAG                
MaeII         215  ACGT                 A^CGT
             1123  ACGT                
MaeIII        550  GTGAC                ^GT[ACGT]AC
              686  GTTAC               
MseI          655  TTAA                 T^TAA
              772  TTAA                
MspA1I         26  CCGCTG               C[AC]G^C[GT]G
             1187  CAGCTG              
PleI           56  GACTC                GAGTC(4/5)
             1204  GACTC               
StyI          590  CCAAGG               C^C[AT][AT]GG
              678  CCATGG              
Tru9I         655  TTAA                 T^TAA
              772  TTAA                
TseI           28  GCTGC                G^C[AT]GC
               75  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       BcnI        BmyI        BpmI        BsaI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MboI        MboII       MflI        MnlI        
MslI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PpuMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TfiI        
Tsp509I     TspRI       XcmI        XhoII       
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