Restriction map for pF1KB5207
 (Linear) (Six-base) MAPSORT of: KIBB5207.seq  Check: 6259  from: 1  to: 1587



KIBB5207.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1477    1587 
   Size:       1477     110
 
AatII G_ACGT'C

Cuts at:      0     218    1587 
   Size:        218    1369
 
Acc65I G'GTAC_C

Cuts at:      0    1443    1587 
   Size:       1443     144
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     861    1471    1587 
   Size:         50     811     610     116

  Fragments arranged by size: 

                 811     610     116      50
 
AfeI AGC'GCT

Cuts at:      0     797    1587 
   Size:        797     790
 
ApaLI G'TGCA_C

Cuts at:      0     386    1367    1587 
   Size:        386     981     220

  Fragments arranged by size: 

                 981     386     220
 
ApoI r'AATT_y

Cuts at:      0     398     542    1587 
   Size:        398     144    1045

  Fragments arranged by size: 

                1045     398     144
 
AvaI C'yCGr_G

Cuts at:      0     839    1587 
   Size:        839     748
 
BamHI G'GATC_C

Cuts at:      0    1194    1224    1587 
   Size:       1194      30     363

  Fragments arranged by size: 

                1194     363      30
 
BanI G'GyrC_C

Cuts at:      0     169    1443    1587 
   Size:        169    1274     144

  Fragments arranged by size: 

                1274     169     144
 
BbeI G_GCGC'C

Cuts at:      0     173    1587 
   Size:        173    1414
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1352    1410    1587 
   Size:       1352      58     177

  Fragments arranged by size: 

                1352     177      58
 
BbvCI CC'TCA_GC

Cuts at:      0     503    1412    1587 
   Size:        503     909     175

  Fragments arranged by size: 

                 909     503     175
 
BclI T'GATC_A

Cuts at:      0     644    1587 
   Size:        644     943
 
BglI GCCn_nnn'nGGC

Cuts at:      0      43    1587 
   Size:         43    1544
 
BlpI GC'TnA_GC

Cuts at:      0      86    1169    1587 
   Size:         86    1083     418

  Fragments arranged by size: 

                1083     418      86
 
Bme1580I G_kGCm'C

Cuts at:      0     390     840    1371    1587 
   Size:        390     450     531     216

  Fragments arranged by size: 

                 531     450     390     216
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     863    1587 
   Size:        863     724
 
BmtI G_CTAG'C

Cuts at:      0     580    1587 
   Size:        580    1007
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     494     644    1285    1587 
   Size:        494     150     641     302

  Fragments arranged by size: 

                 641     494     302     150
 
Bpu10I CC'TnA_GC

Cuts at:      0     503    1412    1587 
   Size:        503     909     175

  Fragments arranged by size: 

                 909     503     175
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     268     875    1450    1587 
   Size:        268     607     575     137

  Fragments arranged by size: 

                 607     575     268     137
 
BsaHI Gr'CG_yC

Cuts at:      0     170     215    1587 
   Size:        170      45    1372

  Fragments arranged by size: 

                1372     170      45
 
BseYI C'CCAG_C

Cuts at:      0      81     299    1173    1587 
   Size:         81     218     874     414

  Fragments arranged by size: 

                 874     414     218      81
 
BsiHKAI G_wGCw'C

Cuts at:      0     319     390     834    1371    1587 
   Size:        319      71     444     537     216

  Fragments arranged by size: 

                 537     444     319     216      71
 
BsmI GAATG_Cn'

Cuts at:      0    1578    1587 
   Size:       1578       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     319     390     834     840    1371    1587 
   Size:        319      71     444       6     531     216

  Fragments arranged by size: 

                 531     444     319     216      71       6
 
BspHI T'CATG_A

Cuts at:      0     431    1587 
   Size:        431    1156
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1477    1587 
   Size:       1477     110
 
BsrBI CCG'CTC

Cuts at:      0     106    1587 
   Size:        106    1481
 
BsrDI GCAATG_nn'

Cuts at:      0     735    1163    1310    1587 
   Size:        735     428     147     277

  Fragments arranged by size: 

                 735     428     277     147
 
BsrGI T'GTAC_A

Cuts at:      0    1068    1587 
   Size:       1068     519
 
BssSI C'ACGA_G

Cuts at:      0     833    1587 
   Size:        833     754
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1014    1587 
   Size:       1014     573
 
BstYI r'GATC_y

Cuts at:      0    1054    1194    1224    1277    1587 
   Size:       1054     140      30      53     310

  Fragments arranged by size: 

                1054     310     140      53      30
 
BtgI C'CryG_G

Cuts at:      0     678    1263    1587 
   Size:        678     585     324

  Fragments arranged by size: 

                 678     585     324
 
BtsI GCAGTG_nn'

Cuts at:      0     212    1248    1587 
   Size:        212    1036     339

  Fragments arranged by size: 

                1036     339     212
 
DraIII CAC_nnn'GTG

Cuts at:      0     829    1587 
   Size:        829     758
 
EaeI y'GGCC_r

Cuts at:      0      23    1004    1587 
   Size:         23     981     583

  Fragments arranged by size: 

                 981     583      23
 
EarI CTCTTCn'nnn_

Cuts at:      0    1568    1587 
   Size:       1568      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     494     644     861    1285    1471    1587 
   Size:         50     444     150     217     424     186     116

  Fragments arranged by size: 

                 444     424     217     186     150     116      50
 
EcoO109I rG'GnC_Cy

Cuts at:      0      44     466     716    1531    1587 
   Size:         44     422     250     815      56

  Fragments arranged by size: 

                 815     422     250      56      44
 
EcoRV GAT'ATC

Cuts at:      0    1308    1587 
   Size:       1308     279
 
HaeII r_GCGC'y

Cuts at:      0     173     799    1587 
   Size:        173     626     788

  Fragments arranged by size: 

                 788     626     173
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     455     487     769     801    1195    1227    1279    1311 
   Size:        455      32     282      32     394      32      52      32

Cuts at:   1311    1567    1587 
   Size:        256      20

  Fragments arranged by size: 

                 455     394     282     256      52      32      32      32
                  32      20
 
HincII GTy'rAC

Cuts at:      0    1355    1587 
   Size:       1355     232
 
KasI G'GCGC_C

Cuts at:      0     169    1587 
   Size:        169    1418
 
KpnI G_GTAC'C

Cuts at:      0    1447    1587 
   Size:       1447     140
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     133    1587 
   Size:        133    1454
 
MscI TGG'CCA

Cuts at:      0    1006    1587 
   Size:       1006     581
 
MslI CAynn'nnrTG

Cuts at:      0     308     523    1012    1136    1253    1587 
   Size:        308     215     489     124     117     334

  Fragments arranged by size: 

                 489     334     308     215     124     117
 
MspA1I CmG'CkG

Cuts at:      0      28    1189    1587 
   Size:         28    1161     398

  Fragments arranged by size: 

                1161     398      28
 
NarI GG'CG_CC

Cuts at:      0     170    1587 
   Size:        170    1417
 
NcoI C'CATG_G

Cuts at:      0     678    1587 
   Size:        678     909
 
NdeI CA'TA_TG

Cuts at:      0    1430    1587 
   Size:       1430     157
 
NheI G'CTAG_C

Cuts at:      0     576    1587 
   Size:        576    1011
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     306    1587 
   Size:        306    1281
 
PfoI T'CCnGG_A

Cuts at:      0    1301    1587 
   Size:       1301     286
 
PpuMI rG'GwC_Cy

Cuts at:      0     466     716    1531    1587 
   Size:        466     250     815      56

  Fragments arranged by size: 

                 815     466     250      56
 
PshAI GACnn'nnGTC

Cuts at:      0     115    1587 
   Size:        115    1472
 
PstI C_TGCA'G

Cuts at:      0    1286    1587 
   Size:       1286     301
 
PvuII CAG'CTG

Cuts at:      0    1189    1587 
   Size:       1189     398
 
SanDI GG'GwC_CC

Cuts at:      0    1531    1587 
   Size:       1531      56
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1568    1587 
   Size:       1568      19
 
SbfI CC_TGCA'GG

Cuts at:      0    1286    1587 
   Size:       1286     301
 
ScaI AGT'ACT

Cuts at:      0     374    1587 
   Size:        374    1213
 
SexAI A'CCwGG_T

Cuts at:      0     825    1587 
   Size:        825     762
 
SfcI C'TryA_G

Cuts at:      0    1282    1587 
   Size:       1282     305
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      43    1587 
   Size:         43    1544
 
SfoI GGC'GCC

Cuts at:      0     171    1587 
   Size:        171    1416
 
SmaI CCC'GGG

Cuts at:      0     841    1587 
   Size:        841     746
 
StuI AGG'CCT

Cuts at:      0    1516    1587 
   Size:       1516      71
 
StyI C'CwwG_G

Cuts at:      0     590     678    1587 
   Size:        590      88     909

  Fragments arranged by size: 

                 909     590      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     298     949    1587 
   Size:        298     651     638

  Fragments arranged by size: 

                 651     638     298
 
TatI w'GTAC_w

Cuts at:      0     372     639     900    1068    1149    1587 
   Size:        372     267     261     168      81     438

  Fragments arranged by size: 

                 438     372     267     261     168      81
 
Tth111I GACn'n_nGTC

Cuts at:      0    1359    1587 
   Size:       1359     228
 
XbaI T'CTAG_A

Cuts at:      0    1200    1587 
   Size:       1200     387
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     285    1170    1323    1587 
   Size:        285     885     153     264

  Fragments arranged by size: 

                 885     285     264     153
 
XmaI C'CCGG_G

Cuts at:      0     839    1587 
   Size:        839     748
 
XmnI GAAnn'nnTTC

Cuts at:      0     975    1587 
   Size:        975     612
 
ZraI GAC'GTC

Cuts at:      0     216    1587 
   Size:        216    1371
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AfeI    ApaLI     ApoI     AvaI
    BamHI     BanI     BbeI     BbsI    BbvCI     BclI     BglI     BlpI
 Bme1580I     BmrI     BmtI     BpmI   Bpu10I     BsaI    BsaHI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrGI
    BssSI    BstXI    BstYI     BtgI     BtsI   DraIII     EaeI     EarI
  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I   HincII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI
    PflMI     PfoI    PpuMI    PshAI     PstI    PvuII    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SmaI     StuI
     StyI    TaqII     TatI  Tth111I     XbaI     XcmI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AccI     AclI    AflII   AflIII     AgeI     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI
    BanII     BcgI    BciVI    BfrBI    BglII    BmgBI     BplI    BpuEI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BsrFI   BssHII   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI  HindIII
     HpaI     MfeI     MluI     NaeI   NgoMIV     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TstI     XhoI
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