Restriction map for pF1KB3400
 (Circular) (Six-base) MAPSORT of: pF1KB3400.seq  Check: 6334  from: 1  to: 3428



pF1KB3400  3428 bp

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:10  ..

AccI GT'mk_AC

Cuts at:    466     466 
   Size:       3428
 
Acc65I G'GTAC_C

Cuts at:    444     444 
   Size:       3428
 
AclI AA'CG_TT

Cuts at:   3268    3268 
   Size:       3428
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1253    1685    2038    2700    1253 
   Size:        432     353     662    1981

  Fragments arranged by size: 

                1981     662     432     353
 
AflIII A'CryG_T

Cuts at:    780    2580    2861     780 
   Size:       1800     281    1347

  Fragments arranged by size: 

                1800    1347     281
 
AlwNI CAG_nnn'CTG

Cuts at:    733    2171     733 
   Size:       1438    1990
 
ApaLI G'TGCA_C

Cuts at:   2266    2266 
   Size:       3428
 
ApoI r'AATT_y

Cuts at:    432    2781    3037     432 
   Size:       2349     256     823

  Fragments arranged by size: 

                2349     823     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3428
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3428
 
AvaI C'yCGr_G

Cuts at:    448     448 
   Size:       3428
 
BamHI G'GATC_C

Cuts at:    453     453 
   Size:       3428
 
BanI G'GyrC_C

Cuts at:    444     858    1106    1141     444 
   Size:        414     248      35    2731

  Fragments arranged by size: 

                2731     414     248      35
 
BanII G_rGCy'C

Cuts at:    442    1472     442 
   Size:       1030    2398
 
BbeI G_GCGC'C

Cuts at:    862    1110     862 
   Size:        248    3180
 
BbsI GAAGACnn'nnnn_

Cuts at:    232     232 
   Size:       3428
 
BciVI GTATCCnnnnn_n'

Cuts at:    238     288    1326    2382    3428     238 
   Size:         50    1038    1056    1046     238

  Fragments arranged by size: 

                1056    1046    1038     238      50
 
BclI T'GATC_A

Cuts at:    948     948 
   Size:       3428
 
BglII A'GATC_T

Cuts at:    943    2980     943 
   Size:       2037    1391
 
BlpI GC'TnA_GC

Cuts at:    540     540 
   Size:       3428
 
Bme1580I G_kGCm'C

Cuts at:   1053    1146    2270    1053 
   Size:         93    1124    2211

  Fragments arranged by size: 

                2211    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    150     203     640     721    1056    3200     150 
   Size:         53     437      81     335    2144     378

  Fragments arranged by size: 

                2144     437     378     335      81      53
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1795    1795 
   Size:       3428
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    604    1483    2197    2495     604 
   Size:        879     714     298    1537

  Fragments arranged by size: 

                1537     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    217    3132     217 
   Size:       2915     513
 
BsaAI yAC'GTr

Cuts at:    781     781 
   Size:       3428
 
BsaBI GATnn'nnATC

Cuts at:      6     942    2979       6 
   Size:        936    2037     455

  Fragments arranged by size: 

                2037     936     455
 
BsaHI Gr'CG_yC

Cuts at:    859    1107    1809    3305     859 
   Size:        248     702    1496     982

  Fragments arranged by size: 

                1496     982     702     248
 
BsaWI w'CCGG_w

Cuts at:    617    1138    1816    2227    2374    2648     617 
   Size:        521     678     411     147     274    1397

  Fragments arranged by size: 

                1397     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    853    1611    2276    2684     853 
   Size:        758     665     408    1597

  Fragments arranged by size: 

                1597     758     665     408
 
BsiEI CG_ry'CG

Cuts at:    104     970    1016    2246     104 
   Size:        866      46    1230    1286

  Fragments arranged by size: 

                1286    1230     866      46
 
BsiHKAI G_wGCw'C

Cuts at:    442    1220    1410    2270     442 
   Size:        778     190     860    1600

  Fragments arranged by size: 

                1600     860     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    442    1053    1146    1220    1410    1472    2270     442 
   Size:        611      93      74     190      62     798    1600

  Fragments arranged by size: 

                1600     798     611     190      93      74      62
 
BspHI T'CATG_A

Cuts at:   1860    1860 
   Size:       3428
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    478     692     994    1375     478 
   Size:        214     302     381    2531

  Fragments arranged by size: 

                2531     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1720    1774    3258    3423      50 
   Size:       1670      54    1484     165      55

  Fragments arranged by size: 

                1670    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1340    1340 
   Size:       3428
 
BsrFI r'CCGG_y

Cuts at:   1426    1607    1426 
   Size:        181    3247
 
BssSI C'ACGA_G

Cuts at:   1699    2407    1699 
   Size:        708    2720
 
BstAPI GCAn_nnn'nTGC

Cuts at:    805     805 
   Size:       3428
 
BstBI TT'CG_AA

Cuts at:   1789    1789 
   Size:       3428
 
BstYI r'GATC_y

Cuts at:      1     453     920     943    1278    1524    1830    1842    1928 
   Size:        452     467      23     335     246     306      12      86

Cuts at:   1928    2980       1 
   Size:       1052     449

  Fragments arranged by size: 

                1052     467     452     449     335     306     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     207     107 
   Size:        100    3328
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1378    1559    1722    1378 
   Size:        181     163    3084

  Fragments arranged by size: 

                3084     181     163
 
BtsI GCAGTG_nn'

Cuts at:    671     671 
   Size:       3428
 
DraI TTT'AAA

Cuts at:    427     427 
   Size:       3428
 
DrdI GACnn_nn'nnGTC

Cuts at:   1134    2478    1134 
   Size:       1344    2084
 
EaeI y'GGCC_r

Cuts at:   1013    1187    1578    1605    2950    1013 
   Size:        174     391      27    1345    1491

  Fragments arranged by size: 

                1491    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1013    1013 
   Size:       3428
 
EarI CTCTTCn'nnn_

Cuts at:   1451    1661    1451 
   Size:        210    3218
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    237    1636    2378    2524    3238     237 
   Size:       1399     742     146     714     427

  Fragments arranged by size: 

                1399     742     714     427     146
 
EcoICRI GAG'CTC

Cuts at:    440     440 
   Size:       3428
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1253    1685    2038    2700    1253 
   Size:        432     353     662    1981

  Fragments arranged by size: 

                1981     662     432     353
 
EcoO109I rG'GnC_Cy

Cuts at:    237     567     237 
   Size:        330    3098
 
EcoRI G'AATT_C

Cuts at:    432     432 
   Size:       3428
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1447    1447 
   Size:       3428
 
FspI TGC'GCA

Cuts at:   1209    1209 
   Size:       3428
 
HaeII r_GCGC'y

Cuts at:    862    1110    2340    3111     862 
   Size:        248    1230     771    1179

  Fragments arranged by size: 

                1230    1179     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1270    1300    1302    1332    1270 
   Size:         30       2      30    3366

  Fragments arranged by size: 

                3366      30      30       2
 
HincII GTy'rAC

Cuts at:    467     467 
   Size:       3428
 
KasI G'GCGC_C

Cuts at:    858    1106     858 
   Size:        248    3180
 
KpnI G_GTAC'C

Cuts at:    448     448 
   Size:       3428
 
MluI A'CGCG_T

Cuts at:   2861    2861 
   Size:       3428
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2187    2371    2187 
   Size:        184    3244
 
MscI TGG'CCA

Cuts at:   1189    1189 
   Size:       3428
 
MslI CAynn'nnrTG

Cuts at:   1417    1544    1417 
   Size:        127    3301
 
MspA1I CmG'CkG

Cuts at:    539     853    1213    1997    2242     539 
   Size:        314     360     784     245    1725

  Fragments arranged by size: 

                1725     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   1609    1609 
   Size:       3428
 
NarI GG'CG_CC

Cuts at:    859    1107     859 
   Size:        248    3180
 
NcoI C'CATG_G

Cuts at:    107     207     107 
   Size:        100    3328
 
NgoMIV G'CCGG_C

Cuts at:   1607    1607 
   Size:       3428
 
NspI r_CATG'y

Cuts at:    481     981    2584     481 
   Size:        500    1603    1325

  Fragments arranged by size: 

                1603    1325     500
 
PciI A'CATG_T

Cuts at:   2580    2580 
   Size:       3428
 
PmeI GTTT'AAAC

Cuts at:    427     427 
   Size:       3428
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1874    1906    1874 
   Size:         32    3396
 
PstI C_TGCA'G

Cuts at:    475    1160     475 
   Size:        685    2743
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3428
 
PvuII CAG'CTG

Cuts at:    853    1213     853 
   Size:        360    3068
 
RsrII CG'GwC_CG

Cuts at:   1623    1623 
   Size:       3428
 
SacI G_AGCT'C

Cuts at:    442     442 
   Size:       3428
 
SalI G'TCGA_C

Cuts at:    465     465 
   Size:       3428
 
SapI GCTCTTCn'nnn_

Cuts at:   1451    1661    1451 
   Size:        210    3218
 
SbfI CC_TGCA'GG

Cuts at:    475     475 
   Size:       3428
 
ScaI AGT'ACT

Cuts at:   2945    2945 
   Size:       3428
 
SfcI C'TryA_G

Cuts at:     33     471    1156    2124    2315      33 
   Size:        438     685     968     191    1146

  Fragments arranged by size: 

                1146     968     685     438     191
 
SfoI GGC'GCC

Cuts at:    860    1108     860 
   Size:        248    3180
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3428
 
SmaI CCC'GGG

Cuts at:    450     450 
   Size:       3428
 
SmlI C'TyrA_G

Cuts at:    583    1498    2212    2474     583 
   Size:        915     714     262    1537

  Fragments arranged by size: 

                1537     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3428
 
SphI G_CATG'C

Cuts at:    481     981     481 
   Size:        500    2928
 
StyI C'CwwG_G

Cuts at:    107     207     562     916     107 
   Size:        100     355     354    2619

  Fragments arranged by size: 

                2619     355     354     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    100    1011    1144    1603    1812     100 
   Size:        911     133     459     209    1716

  Fragments arranged by size: 

                1716     911     459     209     133
 
TatI w'GTAC_w

Cuts at:    329    2943     329 
   Size:       2614     814
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2945    2977    2945 
   Size:         32    3396
 
Tth111I GACn'n_nGTC

Cuts at:   1225    1225 
   Size:       3428
 
XbaI T'CTAG_A

Cuts at:    459     459 
   Size:       3428
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    214     923     214 
   Size:        709    2719
 
XmaI C'CCGG_G

Cuts at:    448     448 
   Size:       3428
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI
    BsiWI     BsmI    BsmBI    BspEI    BsrGI   BssHII   BstEII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI
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