Restriction map for pF1KB3400
 (Linear) (Six-base) MAPSORT of: KIBB3400.seq  Check: 9415  from: 1  to: 315



KIBB3400.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:10  ..

BbsI GAAGACnn'nnnn_

Cuts at:      0     124     315 
   Size:        124     191
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     130     180     315 
   Size:        130      50     135

  Fragments arranged by size: 

                 135     130      50
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      42      95     315 
   Size:         42      53     220

  Fragments arranged by size: 

                 220      53      42
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     109     315 
   Size:        109     206
 
BtgI C'CryG_G

Cuts at:      0      99     315 
   Size:         99     216
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     129     315 
   Size:        129     186
 
EcoO109I rG'GnC_Cy

Cuts at:      0     129     315 
   Size:        129     186
 
NcoI C'CATG_G

Cuts at:      0      99     315 
   Size:         99     216
 
StyI C'CwwG_G

Cuts at:      0      99     315 
   Size:         99     216
 
TatI w'GTAC_w

Cuts at:      0     221     315 
   Size:        221      94
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     106     315 
   Size:        106     209
 

 Enzymes that do cut:

     BbsI    BciVI     BmrI     BsaI     BtgI     EciI EcoO109I     NcoI
     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI
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