Restriction map for pF1KB3240
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       AccI        AcyI        AgeI        AhdI        
Aor51HI     ApaI        AscI        AseI        AsnI        
AspEI       AspI        AviII       AvrII       BamHI       
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BglI        BlnI        
BsaAI       BsaHI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Cfr10I      CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoT14I     EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
KasI        KspI        MfeI        MluI        MunI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StyI        SwaI        Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

Acc65I       1290  GGTACC               G^GTACC
AccII        2267  CGCG                 CG^CG
AccIII       2039  TCCGGA               T^CCGGA
AclI         1004  AACGTT               AA^CGTT
AflIII       2586  ACATGT               A^C[GA][CT]GT
Alw44I       1961  GTGCAC               G^TGCAC
AlwI           55  GATCC                GGATC(4/5)
AlwNI         683  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2217  CCTTAGG              CC^T[ACGT]AGG
ApaLI        1961  GTGCAC               G^TGCAC
Asp718I      1290  GGTACC               G^GTACC
AspHI        1961  GTGCAC               G[AT]GC[AT]^C
AvaI         1033  CCCGAG               C^[CT]CG[GA]G
BanI         1290  GGTACC               G^G[CT][GA]CC
BglII         240  AGATCT               A^GATCT
BsaBI         880  GATAACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2238  GAGACC               GGTCTC(1/5)
BsaWI        2039  TCCGGA               [AT]^CCGG[AT]
BseAI        2039  TCCGGA               T^CCGGA
BsiHKAI      1961  GTGCAC               G[AT]GC[AT]^C
BsmAI        2238  GAGAC                GTCTC(1/5)
BsoBI        1033  CCCGAG               C^[CT]CG[GA]G
BspDI        1834  ATCGAT               AT^CGAT
BspEI        2039  TCCGGA               T^CCGGA
BspLU11I     2586  ACATGT               A^CATGT
BstUI        2267  CGCG                 CG^CG
BstXI        1164  CCAAATAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2217  CCTTAGG              CC^T[ACGT]AGG
CfoI         2183  GCGC                 GCG^C
ClaI         1834  ATCGAT               AT^CGAT
DraII          90  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI         1637  CCACGG               C^C[GA][CT]GG
Eco81I       2217  CCTTAGG              CC^T[ACGT]AGG
EcoNI        1966  CCTAAGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       90  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         813  GAATTC               G^AATTC
EcoRV          72  GATATC               GAT^ATC
FauI         2090  GCGGG                CCCGC(4/6)
HaeII        2182  AGCGCC               [GA]GCGC^[CT]
HapII        2040  CCGG                 C^CGG
HhaI         2183  GCGC                 GCG^C
HinP1I       2183  GCGC                 G^CGC
HindIII      1868  AAGCTT               A^AGCTT
HpaI         2410  GTTAAC               GTT^AAC
HpaII        2040  CCGG                 C^CGG
KpnI         1290  GGTACC               GGTAC^C
MamI          880  GATAACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         2039  TCCGGA               T^CCGGA
MspA1I       2011  CAGCTG               C[AC]G^C[GT]G
MspI         2040  CCGG                 C^CGG
MvnI         2267  CGCG                 CG^CG
NdeI         2422  CATATG               CA^TATG
PflMI        1648  CCAACTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          90  AGGTCCT              [GA]G^G[AT]CC[CT]
Psp1406I     1004  AACGTT               AA^CGTT
PvuII        2011  CAGCTG               CAG^CTG
SspI          218  AATATT               AAT^ATT
StuI         1503  AGGCCT               AGG^CCT
Van91I       1648  CCAACTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AflII        2023  CTTAAG               C^TTAAG
             2353  CTTAAG              
Asp700I       148  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1059  GAAACAATTC          
BalI         2050  TGGCCA               TGG^CCA
             2078  TGGCCA              
BbsI         2261  GAAGAC               GAAGAC(2/6)
             2211  GTCTTC              
BfaI         2099  CTAG                 C^TAG
             2279  CTAG                
BfrI         2023  CTTAAG               C^TTAAG
             2353  CTTAAG              
BlpI          805  GCTCAGC              GC^T[ACGT]AGC
             1300  GCTCAGC             
BmyI         1805  GGGCAC               G[AGT]GC[ACT]^C
             1961  GTGCAC              
Bpu1102I      805  GCTCAGC              GC^T[ACGT]AGC
             1300  GCTCAGC             
BpuAI        2261  GAAGAC               GAAGAC(2/6)
             2211  GTCTTC              
BsgI         1661  CTGCAC               GTGCAG(16/14)
             2014  CTGCAC              
Bsp1286I     1805  GGGCAC               G[AGT]GC[ACT]^C
             1961  GTGCAC              
BspHI         156  TCATGA               T^CATGA
              548  TCATGA              
BstYI          54  AGATCC               [GA]^GATC[CT]
              240  AGATCT              
CelII         805  GCTCAGC              GC^T[ACGT]AGC
             1300  GCTCAGC             
EaeI         2050  TGGCCA               [CT]^GGCC[GA]
             2078  TGGCCA              
HincII        396  GTTGAC               GT[CT]^[GA]AC
             2410  GTTAAC              
HindII        396  GTTGAC               GT[CT]^[GA]AC
             2410  GTTAAC              
MaeI         2099  CTAG                 C^TAG
             2279  CTAG                
MflI           54  AGATCC               [GA]^GATC[CT]
              240  AGATCT              
MluNI        2050  TGGCCA               TGG^CCA
             2078  TGGCCA              
MscI         2050  TGGCCA               TGG^CCA
             2078  TGGCCA              
PleI         1029  GACTC                GAGTC(4/5)
             1621  GACTC               
RcaI          156  TCATGA               T^CATGA
              548  TCATGA              
ScaI          703  AGTACT               AGT^ACT
              733  AGTACT              
SfaNI         343  GATGC                GCATC(5/9)
             1837  GATGC               
TaqI          314  TCGA                 T^CGA
             1835  TCGA                
TthHB8I       314  TCGA                 T^CGA
             1835  TCGA                
XhoII          54  AGATCC               [GA]^GATC[CT]
              240  AGATCT              
XmnI          148  GAAGAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1059  GAAACAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BbvI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmFI       BsmI        BspMI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        SmlI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       
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