Restriction map for pF1KB3240
 (Linear) (Six-base) MAPSORT of: KIBB3240.seq  Check: 5449  from: 1  to: 2619



KIBB3240.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:10  ..

Acc65I G'GTAC_C

Cuts at:      0    1290    2619 
   Size:       1290    1329
 
AclI AA'CG_TT

Cuts at:      0    1005    2619 
   Size:       1005    1614
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     665    1732    2389    2619 
   Size:        641      24    1067     657     230

  Fragments arranged by size: 

                1067     657     641     230      24
 
AflII C'TTAA_G

Cuts at:      0    2023    2353    2619 
   Size:       2023     330     266

  Fragments arranged by size: 

                2023     330     266
 
AflIII A'CryG_T

Cuts at:      0    2586    2619 
   Size:       2586      33
 
AleI CACnn'nnGTG

Cuts at:      0     748    2619 
   Size:        748    1871
 
AlwNI CAG_nnn'CTG

Cuts at:      0     688    2619 
   Size:        688    1931
 
ApaLI G'TGCA_C

Cuts at:      0    1961    2619 
   Size:       1961     658
 
ApoI r'AATT_y

Cuts at:      0     294     387     813    1560    2329    2619 
   Size:        294      93     426     747     769     290

  Fragments arranged by size: 

                 769     747     426     294     290      93
 
AvaI C'yCGr_G

Cuts at:      0    1033    2619 
   Size:       1033    1586
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     782     815    2619 
   Size:        782      33    1804

  Fragments arranged by size: 

                1804     782      33
 
BanI G'GyrC_C

Cuts at:      0    1290    2619 
   Size:       1290    1329
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2204    2268    2619 
   Size:       2204      64     351

  Fragments arranged by size: 

                2204     351      64
 
BglII A'GATC_T

Cuts at:      0     240    2619 
   Size:        240    2379
 
BlpI GC'TnA_GC

Cuts at:      0     806    1301    2619 
   Size:        806     495    1318

  Fragments arranged by size: 

                1318     806     495
 
Bme1580I G_kGCm'C

Cuts at:      0    1809    1965    2619 
   Size:       1809     156     654

  Fragments arranged by size: 

                1809     654     156
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2313    2619 
   Size:       2313     306
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     153    2619 
   Size:        153    2466
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     221    1608    1952    2619 
   Size:        221    1387     344     667

  Fragments arranged by size: 

                1387     667     344     221
 
Bpu10I CC'TnA_GC

Cuts at:      0    1670    2006    2619 
   Size:       1670     336     613

  Fragments arranged by size: 

                1670     613     336
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     123     941    1181    2619 
   Size:         85      38     818     240    1438

  Fragments arranged by size: 

                1438     818     240      85      38
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2232    2619 
   Size:       2232     387
 
BsaBI GATnn'nnATC

Cuts at:      0     884    2619 
   Size:        884    1735
 
BsaWI w'CCGG_w

Cuts at:      0    2039    2619 
   Size:       2039     580
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1122    1152    2619 
   Size:       1122      30    1467

  Fragments arranged by size: 

                1467    1122      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     493    1060    2521    2619 
   Size:        493     567    1461      98

  Fragments arranged by size: 

                1461     567     493      98
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1646    1999    2619 
   Size:       1646     353     620

  Fragments arranged by size: 

                1646     620     353
 
BsiHKAI G_wGCw'C

Cuts at:      0    1965    2619 
   Size:       1965     654
 
BsmI GAATG_Cn'

Cuts at:      0     322     489     854    1397    2619 
   Size:        322     167     365     543    1222

  Fragments arranged by size: 

                1222     543     365     322     167
 
Bsp1286I G_dGCh'C

Cuts at:      0    1809    1965    2619 
   Size:       1809     156     654

  Fragments arranged by size: 

                1809     654     156
 
BspEI T'CCGG_A

Cuts at:      0    2039    2619 
   Size:       2039     580
 
BspHI T'CATG_A

Cuts at:      0     156     548    2619 
   Size:        156     392    2071

  Fragments arranged by size: 

                2071     392     156
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1302    1900    2427    2619 
   Size:       1302     598     527     192

  Fragments arranged by size: 

                1302     598     527     192
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1171    2619 
   Size:       1171    1448
 
BstYI r'GATC_y

Cuts at:      0      54     240    2619 
   Size:         54     186    2379

  Fragments arranged by size: 

                2379     186      54
 
Bsu36I CC'TnA_GG

Cuts at:      0    2218    2619 
   Size:       2218     401
 
BtgI C'CryG_G

Cuts at:      0    1637    2619 
   Size:       1637     982
 
ClaI AT'CG_AT

Cuts at:      0    1835    2619 
   Size:       1835     784
 
DraI TTT'AAA

Cuts at:      0    1010    1716    1874    2334    2619 
   Size:       1010     706     158     460     285

  Fragments arranged by size: 

                1010     706     460     285     158
 
EaeI y'GGCC_r

Cuts at:      0    2050    2078    2619 
   Size:       2050      28     541

  Fragments arranged by size: 

                2050     541      28
 
EarI CTCTTCn'nnn_

Cuts at:      0     143     377     617    1821    1874    2057    2250    2531 
   Size:        143     234     240    1204      53     183     193     281

Cuts at:   2531    2619 
   Size:         88

  Fragments arranged by size: 

                1204     281     240     234     193     183     143      88
                  53
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     221     641     665    1608    1732    1952    2389    2619 
   Size:        221     420      24     943     124     220     437     230

  Fragments arranged by size: 

                 943     437     420     230     221     220     124      24
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1970    2619 
   Size:       1970     649
 
EcoO109I rG'GnC_Cy

Cuts at:      0      91    2619 
   Size:         91    2528
 
EcoRI G'AATT_C

Cuts at:      0     813    2619 
   Size:        813    1806
 
EcoRV GAT'ATC

Cuts at:      0      74    2619 
   Size:         74    2545
 
HaeII r_GCGC'y

Cuts at:      0    2186    2619 
   Size:       2186     433
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      58      90    2306    2338    2619 
   Size:         58      32    2216      32     281

  Fragments arranged by size: 

                2216     281      58      32      32
 
HincII GTy'rAC

Cuts at:      0     398    2412    2619 
   Size:        398    2014     207

  Fragments arranged by size: 

                2014     398     207
 
HindIII A'AGCT_T

Cuts at:      0    1868    2619 
   Size:       1868     751
 
HpaI GTT'AAC

Cuts at:      0    2412    2619 
   Size:       2412     207
 
KpnI G_GTAC'C

Cuts at:      0    1294    2619 
   Size:       1294    1325
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1006    1366    2619 
   Size:       1006     360    1253

  Fragments arranged by size: 

                1253    1006     360
 
MscI TGG'CCA

Cuts at:      0    2052    2080    2619 
   Size:       2052      28     539

  Fragments arranged by size: 

                2052     539      28
 
MslI CAynn'nnrTG

Cuts at:      0     281     553     748    2619 
   Size:        281     272     195    1871

  Fragments arranged by size: 

                1871     281     272     195
 
MspA1I CmG'CkG

Cuts at:      0    2013    2619 
   Size:       2013     606
 
NdeI CA'TA_TG

Cuts at:      0    2423    2619 
   Size:       2423     196
 
NspI r_CATG'y

Cuts at:      0    1163    2022    2590    2619 
   Size:       1163     859     568      29

  Fragments arranged by size: 

                1163     859     568      29
 
PciI A'CATG_T

Cuts at:      0    2586    2619 
   Size:       2586      33
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1654    2619 
   Size:       1654     965
 
PfoI T'CCnGG_A

Cuts at:      0     771    1678    2619 
   Size:        771     907     941

  Fragments arranged by size: 

                 941     907     771
 
PpuMI rG'GwC_Cy

Cuts at:      0      91    2619 
   Size:         91    2528
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1065    1097    2619 
   Size:       1065      32    1522

  Fragments arranged by size: 

                1522    1065      32
 
PstI C_TGCA'G

Cuts at:      0    1314    1731    1950    2619 
   Size:       1314     417     219     669

  Fragments arranged by size: 

                1314     669     417     219
 
PvuII CAG'CTG

Cuts at:      0    2013    2619 
   Size:       2013     606
 
ScaI AGT'ACT

Cuts at:      0     705     735    2619 
   Size:        705      30    1884

  Fragments arranged by size: 

                1884     705      30
 
SfcI C'TryA_G

Cuts at:      0     110     503    1235    1310    1727    1946    2619 
   Size:        110     393     732      75     417     219     673

  Fragments arranged by size: 

                 732     673     417     393     219     110      75
 
SmlI C'TyrA_G

Cuts at:      0      64     138     956    1196    2023    2353    2619 
   Size:         64      74     818     240     827     330     266

  Fragments arranged by size: 

                 827     818     330     266     240      74      64
 
SspI AAT'ATT

Cuts at:      0     220    2619 
   Size:        220    2399
 
StuI AGG'CCT

Cuts at:      0    1505    2619 
   Size:       1505    1114
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     389    2619 
   Size:        389    2230
 
TatI w'GTAC_w

Cuts at:      0     223     703     733    1773    1978    2619 
   Size:        223     480      30    1040     205     641

  Fragments arranged by size: 

                1040     641     480     223     205      30
 
XmnI GAAnn'nnTTC

Cuts at:      0     152    1063    2619 
   Size:        152     911    1556

  Fragments arranged by size: 

                1556     911     152
 

 Enzymes that do cut:

   Acc65I     AclI     AcuI    AflII   AflIII     AleI    AlwNI    ApaLI
     ApoI     AvaI     BaeI     BanI     BbsI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI
    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BstXI    BstYI   Bsu36I     BtgI     ClaI     DraI     EaeI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MmeI     MscI     MslI   MspA1I
     NdeI     NspI     PciI    PflMI     PfoI    PpuMI     PsrI     PstI
    PvuII     ScaI     SfcI     SmlI     SspI     StuI    TaqII     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AfeI     AgeI     AhdI     AlfI     AloI
     ApaI     AscI     AseI    AsiSI    AvrII    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BmgBI     BmtI
    BsaAI    BsaHI    BseYI    BsiEI    BsiWI    BsmBI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
    BtgZI     BtsI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI
     FalI     FseI     FspI    FspAI     KasI     MfeI     MluI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI
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