Restriction map for pF1KB3126
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
AhdI        Aor51HI     ApaI        AscI        Asp718I     
AspEI       AvaI        AviII       AvrII       BbrPI       
BcgI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsiEI       BsiWI       
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       CelII       
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       FauI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MamI        MluI        NaeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       Sse8387I    SspBI       
SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII        2026  GACGTC               GACGT^C
AccI          419  GTATAC               GT^[AC][GT]AC
AccII        1557  CGCG                 CG^CG
AccIII        660  TCCGGA               T^CCGGA
Alw44I        511  GTGCAC               G^TGCAC
AocI          244  CCTCAGG              CC^T[ACGT]AGG
ApaLI         511  GTGCAC               G^TGCAC
AseI          961  ATTAAT               AT^TAAT
AsnI          961  ATTAAT               AT^TAAT
Asp700I        85  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI         2333  TGGCCA               TGG^CCA
BamHI          51  GGATCC               G^GATCC
BbeI          686  GGCGCC               GGCGC^C
BciVI        2525  GGATAC               GTATCC(6/5)
BclI         1769  TGATCA               T^GATCA
BcnI         2051  CCGGG                CC^[GC]GG
BglI         2014  GCCGCTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI         2589  GGTCTC               GGTCTC(1/5)
BsaWI         660  TCCGGA               [AT]^CCGG[AT]
BseAI         660  TCCGGA               T^CCGGA
BseRI        1178  GAGGAG               GAGGAG(10/8)
BsmBI        1568  GAGACG               CGTCTC(1/5)
BsmI         1956  GAATGC               GAATGC(1/-1)
BspEI         660  TCCGGA               T^CCGGA
BsrFI         554  ACCGGC               [GA]^CCGG[CT]
Bst1107I      419  GTATAC               GTA^TAC
BstUI        1557  CGCG                 CG^CG
BstZ17I       419  GTATAC               GTA^TAC
Bsu36I        244  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        554  ACCGGC               [GA]^CCGG[CT]
DrdI          391  GACATGGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        244  CCTCAGG              CC^T[ACGT]AGG
EcoT22I       737  ATGCAT               ATGCA^T
FbaI         1769  TGATCA               T^GATCA
KasI          686  GGCGCC               G^GCGCC
MfeI         2651  CAATTG               C^AATTG
MluNI        2333  TGGCCA               TGG^CCA
MroI          660  TCCGGA               T^CCGGA
MscI         2333  TGGCCA               TGG^CCA
MunI         2651  CAATTG               C^AATTG
MvnI         1557  CGCG                 CG^CG
NarI          686  GGCGCC               GG^CGCC
NciI         2051  CCGGG                CC^[GC]GG
NdeI         2375  CATATG               CA^TATG
NsiI          737  ATGCAT               ATGCA^T
PshBI         961  ATTAAT               AT^TAAT
SacI         2749  GAGCTC               GAGCT^C
SapI         2206  GCTCTTC              GCTCTTC(1/4)
ScaI         2140  AGTACT               AGT^ACT
SexAI         207  ACCTGGT              A^CC[AT]GGT
SfoI          686  GGCGCC               GGC^GCC
SmlI         1903  CTCAAG               C^T[CT][GA]AG
SpeI         1021  ACTAGT               A^CTAGT
StuI           28  AGGCCT               AGG^CCT
XmnI           85  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI          800  AAATTT               [GA]^AATT[CT]
             2471  AAATTC              
AlwNI         977  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2766  CAGTGTCTG           
ApoI          800  AAATTT               [GA]^AATT[CT]
             2471  AAATTC              
AspI         1630  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2764  GACAGTGTC           
BanII        1408  GAGCCC               G[GA]GC[CT]^C
             2749  GAGCTC              
BbsI         2685  GAAGAC               GAAGAC(2/6)
              934  GTCTTC              
BbvCI         624  CCTCAGC              CCTCAGC(-5/-2)
             1803  GCTGAGG             
BpuAI        2685  GAAGAC               GAAGAC(2/6)
              934  GTCTTC              
BspMI         320  ACCTGC               ACCTGC(4/8)
             2346  GCAGGT              
EcoNI        2726  CCTCCACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2887  CCTCTAGAAGG         
HaeII         686  GGCGCC               [GA]GCGC^[CT]
             1595  AGCGCC              
HgaI          709  GACGC                GACGC(5/10)
             1749  GACGC               
NcoI          195  CCATGG               C^CATGG
             1301  CCATGG              
PflFI        1630  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2764  GACAGTGTC           
PpuMI        1322  AGGACCC              [GA]G^G[AT]CC[CT]
             2883  AGGACCT             
PstI          601  CTGCAG               CTGCA^G
             2449  CTGCAG              
SfcI          601  CTGCAG               C^T[GA][CT]AG
             2449  CTGCAG              
SphI          324  GCATGC               GCATG^C
             1533  GCATGC              
TaqI          846  TCGA                 T^CGA
             1550  TCGA                
TfiI          843  GATTC                G^A[AT]TC
             1312  GAATC               
Tth111I      1630  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2764  GACAGTGTC           
TthHB8I       846  TCGA                 T^CGA
             1550  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AspHI       AvaII       BanI        BbvI        BfaI        
BmyI        BpmI        BsaHI       BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SspI        StyI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XbaI        
XcmI        XhoII       
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