Restriction map for pF1KB3126
 (Linear) (Six-base) MAPSORT of: KIBB3126.seq  Check: 2303  from: 1  to: 2904



KIBB3126.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     329    2904 
   Size:        329    2575
 
AatII G_ACGT'C

Cuts at:      0    2030    2904 
   Size:       2030     874
 
AccI GT'mk_AC

Cuts at:      0     420    2904 
   Size:        420    2484
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1349    2456    2904 
   Size:       1349    1107     448

  Fragments arranged by size: 

                1349    1107     448
 
AleI CACnn'nnGTG

Cuts at:      0    1609    2904 
   Size:       1609    1295
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1556    1880    2005    2080    2904 
   Size:       1556     324     125      75     824

  Fragments arranged by size: 

                1556     824     324     125      75
 
AlwNI CAG_nnn'CTG

Cuts at:      0     982    2771    2904 
   Size:        982    1789     133

  Fragments arranged by size: 

                1789     982     133
 
ApaLI G'TGCA_C

Cuts at:      0     511    2904 
   Size:        511    2393
 
ApoI r'AATT_y

Cuts at:      0     800    2471    2904 
   Size:        800    1671     433

  Fragments arranged by size: 

                1671     800     433
 
AseI AT'TA_AT

Cuts at:      0     962    2904 
   Size:        962    1942
 
BamHI G'GATC_C

Cuts at:      0      51    2904 
   Size:         51    2853
 
BanI G'GyrC_C

Cuts at:      0     211     686    1135    2833    2904 
   Size:        211     475     449    1698      71

  Fragments arranged by size: 

                1698     475     449     211      71
 
BanII G_rGCy'C

Cuts at:      0    1412    2753    2904 
   Size:       1412    1341     151

  Fragments arranged by size: 

                1412    1341     151
 
BbeI G_GCGC'C

Cuts at:      0     690    2904 
   Size:        690    2214
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     927    2692    2904 
   Size:        927    1765     212

  Fragments arranged by size: 

                1765     927     212
 
BbvCI CC'TCA_GC

Cuts at:      0     625    1804    2904 
   Size:        625    1179    1100

  Fragments arranged by size: 

                1179    1100     625
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2519    2904 
   Size:       2519     385
 
BclI T'GATC_A

Cuts at:      0    1769    2904 
   Size:       1769    1135
 
BfrBI ATG'CAT

Cuts at:      0     739    2904 
   Size:        739    2165
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2020    2904 
   Size:       2020     884
 
Bme1580I G_kGCm'C

Cuts at:      0     515    1189    2904 
   Size:        515     674    1715

  Fragments arranged by size: 

                1715     674     515
 
BmgBI CAC'GTC

Cuts at:      0    1572    2904 
   Size:       1572    1332
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2502    2674    2904 
   Size:       2502     172     230

  Fragments arranged by size: 

                2502     230     172
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2573    2904 
   Size:       2573     331
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     809    1166    1502    2137    2658    2904 
   Size:        809     357     336     635     521     246

  Fragments arranged by size: 

                 809     635     521     357     336     246
 
Bpu10I CC'TnA_GC

Cuts at:      0     625    1804    1822    2036    2904 
   Size:        625    1179      18     214     868

  Fragments arranged by size: 

                1179     868     625     214      18
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1888    2904 
   Size:       1888    1016
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2595    2904 
   Size:       2595     309
 
BsaHI Gr'CG_yC

Cuts at:      0     687     710    1750    2027    2904 
   Size:        687      23    1040     277     877

  Fragments arranged by size: 

                1040     877     687     277      23
 
BsaWI w'CCGG_w

Cuts at:      0     660    2904 
   Size:        660    2244
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2032    2062    2904 
   Size:       2032      30     842

  Fragments arranged by size: 

                2032     842      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1193    2904 
   Size:       1193    1711
 
BseYI C'CCAG_C

Cuts at:      0     561    1282    1411    1810    2017    2179    2904 
   Size:        561     721     129     399     207     162     725

  Fragments arranged by size: 

                 725     721     561     399     207     162     129
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351    1096    1633    2050    2904 
   Size:        351     745     537     417     854

  Fragments arranged by size: 

                 854     745     537     417     351
 
BsiHKAI G_wGCw'C

Cuts at:      0     515    2084    2753    2904 
   Size:        515    1569     669     151

  Fragments arranged by size: 

                1569     669     515     151
 
BsmI GAATG_Cn'

Cuts at:      0    1962    2904 
   Size:       1962     942
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1562    2904 
   Size:       1562    1342
 
Bsp1286I G_dGCh'C

Cuts at:      0     515    1189    1412    2084    2753    2904 
   Size:        515     674     223     672     669     151

  Fragments arranged by size: 

                 674     672     669     515     223     151
 
BspEI T'CCGG_A

Cuts at:      0     660    2904 
   Size:        660    2244
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     329    2337    2904 
   Size:        329    2008     567

  Fragments arranged by size: 

                2008     567     329
 
BsrFI r'CCGG_y

Cuts at:      0     554    2904 
   Size:        554    2350
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     393     618    2199    2872    2904 
   Size:        393     225    1581     673      32

  Fragments arranged by size: 

                1581     673     393     225      32
 
BstYI r'GATC_y

Cuts at:      0      51     362    1680    2229    2904 
   Size:         51     311    1318     549     675

  Fragments arranged by size: 

                1318     675     549     311      51
 
BstZ17I GTA'TAC

Cuts at:      0     421    2904 
   Size:        421    2483
 
Bsu36I CC'TnA_GG

Cuts at:      0     245    2904 
   Size:        245    2659
 
BtgI C'CryG_G

Cuts at:      0     195     698    1301    2904 
   Size:        195     503     603    1603

  Fragments arranged by size: 

                1603     603     503     195
 
BtsI GCAGTG_nn'

Cuts at:      0     106    1604    1772    2062    2839    2904 
   Size:        106    1498     168     290     777      65

  Fragments arranged by size: 

                1498     777     290     168     106      65
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     397    2904 
   Size:        397    2507
 
EaeI y'GGCC_r

Cuts at:      0    1586    2012    2333    2904 
   Size:       1586     426     321     571

  Fragments arranged by size: 

                1586     571     426     321
 
EarI CTCTTCn'nnn_

Cuts at:      0     851    1466    2213    2904 
   Size:        851     615     747     691

  Fragments arranged by size: 

                 851     747     691     615
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     577    1166    2904 
   Size:        577     589    1738

  Fragments arranged by size: 

                1738     589     577
 
EcoICRI GAG'CTC

Cuts at:      0    2751    2904 
   Size:       2751     153
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     809    1166    1349    1502    2137    2456    2658    2904 
   Size:        809     357     183     153     635     319     202     246

  Fragments arranged by size: 

                 809     635     357     319     246     202     183     153
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2730    2891    2904 
   Size:       2730     161      13

  Fragments arranged by size: 

                2730     161      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1323    2622    2884    2904 
   Size:       1323    1299     262      20

  Fragments arranged by size: 

                1323    1299     262      20
 
HaeII r_GCGC'y

Cuts at:      0     690    1599    2904 
   Size:        690     909    1305

  Fragments arranged by size: 

                1305     909     690
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1071    1103    1297    1329    1722    1754    2032    2064 
   Size:       1071      32     194      32     393      32     278      32

Cuts at:   2064    2077    2109    2377    2409    2679    2711    2904 
   Size:         13      32     268      32     270      32     193

  Fragments arranged by size: 

                1071     393     278     270     268     194     193      32
                  32      32      32      32      32      32      13
 
KasI G'GCGC_C

Cuts at:      0     686    2904 
   Size:        686    2218
 
MfeI C'AATT_G

Cuts at:      0    2651    2904 
   Size:       2651     253
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     767    2904 
   Size:        767    2137
 
MscI TGG'CCA

Cuts at:      0    2335    2904 
   Size:       2335     569
 
MslI CAynn'nnrTG

Cuts at:      0     323    1538    1609    2870    2904 
   Size:        323    1215      71    1261      34

  Fragments arranged by size: 

                1261    1215     323      71      34
 
MspA1I CmG'CkG

Cuts at:      0     561     775    2017    2179    2342    2430    2454    2780 
   Size:        561     214    1242     162     163      88      24     326

Cuts at:   2780    2904 
   Size:        124

  Fragments arranged by size: 

                1242     561     326     214     163     162     124      88
                  24
 
NarI GG'CG_CC

Cuts at:      0     687    2904 
   Size:        687    2217
 
NcoI C'CATG_G

Cuts at:      0     195    1301    2904 
   Size:        195    1106    1603

  Fragments arranged by size: 

                1603    1106     195
 
NdeI CA'TA_TG

Cuts at:      0    2376    2904 
   Size:       2376     528
 
NsiI A_TGCA'T

Cuts at:      0     741    2904 
   Size:        741    2163
 
NspI r_CATG'y

Cuts at:      0     328    1161    1537    2904 
   Size:        328     833     376    1367

  Fragments arranged by size: 

                1367     833     376     328
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     118     196     336    1225    2342    2904 
   Size:        118      78     140     889    1117     562

  Fragments arranged by size: 

                1117     889     562     140     118      78
 
PfoI T'CCnGG_A

Cuts at:      0     498    1260    2114    2904 
   Size:        498     762     854     790

  Fragments arranged by size: 

                 854     790     762     498
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2307    2339    2904 
   Size:       2307      32     565

  Fragments arranged by size: 

                2307     565      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1323    2884    2904 
   Size:       1323    1561      20

  Fragments arranged by size: 

                1561    1323      20
 
PstI C_TGCA'G

Cuts at:      0     605    2453    2904 
   Size:        605    1848     451

  Fragments arranged by size: 

                1848     605     451
 
PvuII CAG'CTG

Cuts at:      0     561     775    2179    2342    2430    2454    2904 
   Size:        561     214    1404     163      88      24     450

  Fragments arranged by size: 

                1404     561     450     214     163      88      24
 
SacI G_AGCT'C

Cuts at:      0    2753    2904 
   Size:       2753     151
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2213    2904 
   Size:       2213     691
 
ScaI AGT'ACT

Cuts at:      0    2142    2904 
   Size:       2142     762
 
SexAI A'CCwGG_T

Cuts at:      0     207    2904 
   Size:        207    2697
 
SfcI C'TryA_G

Cuts at:      0     601    2449    2904 
   Size:        601    1848     455

  Fragments arranged by size: 

                1848     601     455
 
SfoI GGC'GCC

Cuts at:      0     688    2904 
   Size:        688    2216
 
SmlI C'TyrA_G

Cuts at:      0    1903    2904 
   Size:       1903    1001
 
SpeI A'CTAG_T

Cuts at:      0    1021    2904 
   Size:       1021    1883
 
SphI G_CATG'C

Cuts at:      0     328    1537    2904 
   Size:        328    1209    1367

  Fragments arranged by size: 

                1367    1209     328
 
SspI AAT'ATT

Cuts at:      0      74     137     966    2904 
   Size:         74      63     829    1938

  Fragments arranged by size: 

                1938     829      74      63
 
StuI AGG'CCT

Cuts at:      0      30    2904 
   Size:         30    2874
 
StyI C'CwwG_G

Cuts at:      0     195     386     611    1301    2904 
   Size:        195     191     225     690    1603

  Fragments arranged by size: 

                1603     690     225     195     191
 
TatI w'GTAC_w

Cuts at:      0    1195    2140    2904 
   Size:       1195     945     764

  Fragments arranged by size: 

                1195     945     764
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     494     526    2904 
   Size:        494      32    2378

  Fragments arranged by size: 

                2378     494      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1633    2767    2904 
   Size:       1633    1134     137

  Fragments arranged by size: 

                1633    1134     137
 
XbaI T'CTAG_A

Cuts at:      0    1503    2325    2889    2904 
   Size:       1503     822     564      15

  Fragments arranged by size: 

                1503     822     564      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     393    2489    2571    2904 
   Size:        393    2096      82     333

  Fragments arranged by size: 

                2096     393     333      82
 
XmnI GAAnn'nnTTC

Cuts at:      0      89    2904 
   Size:         89    2815
 
ZraI GAC'GTC

Cuts at:      0    2028    2904 
   Size:       2028     876
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AleI     AlfI    AlwNI    ApaLI
     ApoI     AseI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
    BciVI     BclI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrFI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NdeI     NsiI     NspI    PflMI     PfoI     PpiI    PpuMI
     PstI    PvuII     SacI     SapI     ScaI    SexAI     SfcI     SfoI
     SmlI     SpeI     SphI     SspI     StuI     StyI     TatI     TstI
  Tth111I     XbaI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AloI
     ApaI     AscI    AsiSI     AvaI    AvrII     BaeI     BcgI    BglII
     BlpI     BmtI    BsaAI    BsaBI    BsiEI    BsiWI    BspHI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BtgZI
     ClaI    CspCI     DraI   DraIII     EagI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MluI
     NaeI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SrfI     SwaI    TaqII     XhoI     XmaI
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