Restriction map for pF1KB5402
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AvrII       BalI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BpmI        BsaBI       BseAI       BsiWI       BsmBI       
BsmFI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoO65I     EcoRI       EcoRV       FbaI        FseI        
HindIII     HpaI        KpnI        MaeI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MunI        NcoI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflMI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        ScaI        SexAI       SfiI        
SfuI        SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        Tru9I       Tsp509I     
Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccI          364  GTCTAC               GT^[AC][GT]AC
AfaI          936  GTAC                 GT^AC
AgeI         1086  ACCGGT               A^CCGGT
ApaI          161  GGGCCC               GGGCC^C
Asp700I       541  GAAATCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          748  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          164  CCCGGG               C^[CT]CG[GA]G
AviII          32  TGCGCA               TGC^GCA
BamHI         680  GGATCC               G^GATCC
BbeI           23  GGCGCC               GGCGC^C
BlpI         1116  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1116  GCTCAGC              GC^T[ACGT]AGC
BsaAI         190  TACGTG               [CT]AC^GT[GA]
BsaI          897  GGTCTC               GGTCTC(1/5)
BsaWI        1086  ACCGGT               [AT]^CCGG[AT]
BsiEI         251  CGGCCG               CG[GA][CT]^CG
BsoBI         164  CCCGGG               C^[CT]CG[GA]G
BspMI         605  ACCTGC               ACCTGC(4/8)
BstAPI        288  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         815  CCATCTGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1116  GCTCAGC              GC^T[ACGT]AGC
DraII         773  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EagI          251  CGGCCG               C^GGCCG
EarI         1005  CTCTTC               CTCTTC(1/4)
EclXI         251  CGGCCG               C^GGCCG
Eco52I        251  CGGCCG               C^GGCCG
EcoO109I      773  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       578  ATGCAT               ATGCA^T
FspI           32  TGCGCA               TGC^GCA
HgaI          245  GCGTC                GACGC(5/10)
HincII        133  GTCAAC               GT[CT]^[GA]AC
HindII        133  GTCAAC               GT[CT]^[GA]AC
HinfI         940  GAATC                G^A[ACGT]TC
KasI           23  GGCGCC               G^GCGCC
Ksp632I      1005  CTCTTC               CTCTTC(1/4)
KspI          358  CCGCGG               CCGC^GG
MaeIII          7  GTCAC                ^GT[ACGT]AC
NarI           23  GGCGCC               GG^CGCC
NsiI          578  ATGCAT               ATGCA^T
NspI         1043  ACATGC               [GA]CATG^[CT]
PflFI         748  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1086  ACCGGT               A^CCGGT
PpuMI         773  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII          28  CAGCTG               CAG^CTG
RsaI          936  GTAC                 GT^AC
SacI         1114  GAGCTC               GAGCT^C
SacII         358  CCGCGG               CCGC^GG
SapI         1004  GCTCTTC              GCTCTTC(1/4)
SbfI          225  CCTGCAGG             CCTGCA^GG
SfoI           23  GGCGCC               GGC^GCC
SgrAI        1085  CACCGGTG             C[GA]^CCGG[CT]G
SmaI          164  CCCGGG               CCC^GGG
Sse8387I      225  CCTGCAGG             CCTGCA^GG
TfiI          940  GAATC                G^A[AT]TC
Tsp45I          7  GTCAC                ^GT[GC]AC
Tth111I       748  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         164  CCCGGG               C^CCGGG
XmaI          164  CCCGGG               C^CCGGG
XmnI          541  GAAATCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI           23  GGCGCC               G[GA]^CG[CT]C
              244  GGCGTC              
AspHI          73  GTGCTC               G[AT]GC[AT]^C
             1114  GAGCTC              
BbsI          691  GAAGAC               GAAGAC(2/6)
              837  GAAGAC              
BcnI          164  CCCGG                CC^[GC]GG
              165  CCGGG               
BpuAI         691  GAAGAC               GAAGAC(2/6)
              837  GAAGAC              
BsaHI          23  GGCGCC               G[GA]^CG[CT]C
              244  GGCGTC              
BsgI          607  CTGCAC               GTGCAG(16/14)
             1059  CTGCAC              
BsiHKAI        73  GTGCTC               G[AT]GC[AT]^C
             1114  GAGCTC              
DsaI          254  CCGTGG               C^C[GA][CT]GG
              358  CCGCGG              
EaeI          251  CGGCCG               [CT]^GGCC[GA]
             1139  CGGCCA              
EcoNI         225  CCTGCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              855  CCTGAAGGAGG         
EcoT14I       561  CCTTGG               C^C[AT][AT]GG
              987  CCAAGG              
HaeII          23  GGCGCC               [GA]GCGC^[CT]
              307  AGCGCC              
Hin1I          23  GGCGCC               G[GA]^CG[CT]C
              244  GGCGTC              
HphI          326  TCACC                GGTGA(8/7)
              808  TCACC               
MslI          581  CATCCAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              816  CATCTGCATG          
MspA1I         28  CAGCTG               C[AC]G^C[GT]G
              358  CCGCGG              
NaeI           83  GCCGGC               GCC^GGC
              352  GCCGGC              
NciI          164  CCCGG                CC^[GC]GG
              165  CCGGG               
NgoMIV         83  GCCGGC               G^CCGGC
              352  GCCGGC              
PstI          226  CTGCAG               CTGCA^G
              931  CTGCAG              
SfaNI          39  GATGC                GCATC(5/9)
              580  GCATC               
StuI          650  AGGCCT               AGG^CCT
              863  AGGCCT              
StyI          561  CCTTGG               C^C[AT][AT]GG
              987  CCAAGG              
XcmI          783  CCACTGTGAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              812  CCACCATCTGCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
AvaII       BanI        BanII       BbvI        BmyI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrFI       BsrI        BssHII      BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MaeII       MboI        MboII       MflI        MnlI        
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SfcI        TaqI        TseI        TspRI       TthHB8I     
XhoII       
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