Restriction map for pF1KB5402
 (Linear) (Six-base) MAPSORT of: KIBB5402.seq  Check: 2814  from: 1  to: 1152



KIBB5402.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AccI GT'mk_AC

Cuts at:      0     365    1152 
   Size:        365     787
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     877    1152 
   Size:        877     275
 
AgeI A'CCGG_T

Cuts at:      0    1086    1152 
   Size:       1086      66
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     603    1152 
   Size:        603     549
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     789     821    1152 
   Size:        789      32     331

  Fragments arranged by size: 

                 789     331      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     294     930    1032    1152 
   Size:        294     636     102     120

  Fragments arranged by size: 

                 636     294     120     102
 
ApaI G_GGCC'C

Cuts at:      0     165    1152 
   Size:        165     987
 
AvaI C'yCGr_G

Cuts at:      0     164    1152 
   Size:        164     988
 
BamHI G'GATC_C

Cuts at:      0     680    1152 
   Size:        680     472
 
BanI G'GyrC_C

Cuts at:      0      23    1051    1083    1089    1152 
   Size:         23    1028      32       6      63

  Fragments arranged by size: 

                1028      63      32      23       6
 
BanII G_rGCy'C

Cuts at:      0     165     468    1039    1118    1152 
   Size:        165     303     571      79      34

  Fragments arranged by size: 

                 571     303     165      79      34
 
BbeI G_GCGC'C

Cuts at:      0      27    1152 
   Size:         27    1125
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     698     844    1152 
   Size:        698     146     308

  Fragments arranged by size: 

                 698     308     146
 
BfrBI ATG'CAT

Cuts at:      0     580    1152 
   Size:        580     572
 
BlpI GC'TnA_GC

Cuts at:      0    1117    1152 
   Size:       1117      35
 
Bme1580I G_kGCm'C

Cuts at:      0     165    1152 
   Size:        165     987
 
BmgBI CAC'GTC

Cuts at:      0      11    1152 
   Size:         11    1141
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     529    1152 
   Size:        529     623
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     903    1152 
   Size:        903     249
 
BsaAI yAC'GTr

Cuts at:      0     192    1152 
   Size:        192     960
 
BsaHI Gr'CG_yC

Cuts at:      0      24     245    1152 
   Size:         24     221     907

  Fragments arranged by size: 

                 907     221      24
 
BsaWI w'CCGG_w

Cuts at:      0    1086    1152 
   Size:       1086      66
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     700     730     758     788    1152 
   Size:        700      30      28      30     364

  Fragments arranged by size: 

                 700     364      30      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     316     900     903    1152 
   Size:        316     584       3     249

  Fragments arranged by size: 

                 584     316     249       3
 
BseYI C'CCAG_C

Cuts at:      0     793     971    1152 
   Size:        793     178     181

  Fragments arranged by size: 

                 793     181     178
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     592    1044    1152 
   Size:        592     452     108

  Fragments arranged by size: 

                 592     452     108
 
BsiEI CG_ry'CG

Cuts at:      0     254    1152 
   Size:        254     898
 
BsiHKAI G_wGCw'C

Cuts at:      0      77    1118    1152 
   Size:         77    1041      34

  Fragments arranged by size: 

                1041      77      34
 
Bsp1286I G_dGCh'C

Cuts at:      0      77     165     468    1039    1118    1152 
   Size:         77      88     303     571      79      34

  Fragments arranged by size: 

                 571     303      88      79      77      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     614    1152 
   Size:        614     538
 
BsrFI r'CCGG_y

Cuts at:      0      83     352    1086    1152 
   Size:         83     269     734      66

  Fragments arranged by size: 

                 734     269      83      66
 
BssHII G'CGCG_C

Cuts at:      0      61     184     211     213     215    1152 
   Size:         61     123      27       2       2     937

  Fragments arranged by size: 

                 937     123      61      27       2       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     294    1152 
   Size:        294     858
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     822    1152 
   Size:        822     330
 
BstYI r'GATC_y

Cuts at:      0     680     797     947    1152 
   Size:        680     117     150     205

  Fragments arranged by size: 

                 680     205     150     117
 
BtgI C'CryG_G

Cuts at:      0     254     358    1152 
   Size:        254     104     794

  Fragments arranged by size: 

                 794     254     104
 
EaeI y'GGCC_r

Cuts at:      0     251    1139    1152 
   Size:        251     888      13

  Fragments arranged by size: 

                 888     251      13
 
EagI C'GGCC_G

Cuts at:      0     251    1152 
   Size:        251     901
 
EarI CTCTTCn'nnn_

Cuts at:      0    1011    1152 
   Size:       1011     141
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     841    1152 
   Size:        841     311
 
EcoICRI GAG'CTC

Cuts at:      0    1116    1152 
   Size:       1116      36
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     877    1152 
   Size:        877     275
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     229     859    1152 
   Size:        229     630     293

  Fragments arranged by size: 

                 630     293     229
 
EcoO109I rG'GnC_Cy

Cuts at:      0     774    1152 
   Size:        774     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     882    1152 
   Size:        882     270
 
FspI TGC'GCA

Cuts at:      0      34    1152 
   Size:         34    1118
 
HaeII r_GCGC'y

Cuts at:      0      27     311    1152 
   Size:         27     284     841

  Fragments arranged by size: 

                 841     284      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     455     487     700     732     758     790    1152 
   Size:        455      32     213      32      26      32     362

  Fragments arranged by size: 

                 455     362     213      32      32      32      26
 
HincII GTy'rAC

Cuts at:      0     135    1152 
   Size:        135    1017
 
KasI G'GCGC_C

Cuts at:      0      23    1152 
   Size:         23    1129
 
MslI CAynn'nnrTG

Cuts at:      0     585     820    1152 
   Size:        585     235     332

  Fragments arranged by size: 

                 585     332     235
 
MspA1I CmG'CkG

Cuts at:      0      30     360    1152 
   Size:         30     330     792

  Fragments arranged by size: 

                 792     330      30
 
NaeI GCC'GGC

Cuts at:      0      85     354    1152 
   Size:         85     269     798

  Fragments arranged by size: 

                 798     269      85
 
NarI GG'CG_CC

Cuts at:      0      24    1152 
   Size:         24    1128
 
NgoMIV G'CCGG_C

Cuts at:      0      83     352    1152 
   Size:         83     269     800

  Fragments arranged by size: 

                 800     269      83
 
NsiI A_TGCA'T

Cuts at:      0     582    1152 
   Size:        582     570
 
NspI r_CATG'y

Cuts at:      0    1047    1152 
   Size:       1047     105
 
PpuMI rG'GwC_Cy

Cuts at:      0     774    1152 
   Size:        774     378
 
PspOMI G'GGCC_C

Cuts at:      0     161    1152 
   Size:        161     991
 
PstI C_TGCA'G

Cuts at:      0     230     935    1152 
   Size:        230     705     217

  Fragments arranged by size: 

                 705     230     217
 
PvuII CAG'CTG

Cuts at:      0      30    1152 
   Size:         30    1122
 
SacI G_AGCT'C

Cuts at:      0    1118    1152 
   Size:       1118      34
 
SacII CC_GC'GG

Cuts at:      0     361    1152 
   Size:        361     791
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1011    1152 
   Size:       1011     141
 
SbfI CC_TGCA'GG

Cuts at:      0     230    1152 
   Size:        230     922
 
SfcI C'TryA_G

Cuts at:      0     226     507     931    1152 
   Size:        226     281     424     221

  Fragments arranged by size: 

                 424     281     226     221
 
SfoI GGC'GCC

Cuts at:      0      25    1152 
   Size:         25    1127
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1086    1152 
   Size:       1086      66
 
SmaI CCC'GGG

Cuts at:      0     166    1152 
   Size:        166     986
 
StuI AGG'CCT

Cuts at:      0     652     865    1152 
   Size:        652     213     287

  Fragments arranged by size: 

                 652     287     213
 
StyI C'CwwG_G

Cuts at:      0     561     987    1152 
   Size:        561     426     165

  Fragments arranged by size: 

                 561     426     165
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     825    1152 
   Size:        825     327
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     476     508    1152 
   Size:        476      32     644

  Fragments arranged by size: 

                 644     476      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     751    1152 
   Size:        751     401
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     790     819    1152 
   Size:        790      29     333

  Fragments arranged by size: 

                 790     333      29
 
XmaI C'CCGG_G

Cuts at:      0     164    1152 
   Size:        164     988
 
XmnI GAAnn'nnTTC

Cuts at:      0     545    1152 
   Size:        545     607
 

 Enzymes that do cut:

     AccI     AcuI     AgeI     AlfI     AloI    AlwNI     ApaI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BfrBI     BlpI Bme1580I
    BmgBI     BmrI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrFI   BssHII
   BstAPI    BstXI    BstYI     BtgI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI     FspI    HaeII    Hin4I
   HincII     KasI     MslI   MspA1I     NaeI     NarI   NgoMIV     NsiI
     NspI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII     SapI
     SbfI     SfcI     SfoI    SgrAI     SmaI     StuI     StyI    TaqII
     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BmtI     BplI
     BpmI   Bpu10I    BpuEI    BsaBI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
    EcoRI    EcoRV     FseI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NcoI     NdeI     NheI     NotI     NruI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI
     ScaI    SexAI     SfiI     SgfI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI     XbaI     XhoI     ZraI
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