Restriction map for pF1KE3504
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AvaI        AviII       AvrII       BclI        BfrI        
BlnI        BsaBI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspEI       
BspHI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       BstEII      BstPI       Bsu36I      
Cfr10I      CpoI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MamI        MluI        MroI        
NaeI        NgoMIV      NheI        NotI        NruI        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SfiI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AhdI         2387  GACCTAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        2132  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI        2387  GACCTAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          124  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         840  GGATCC               G^GATCC
BanII         910  GGGCTC               G[GA]GC[CT]^C
BbrPI         540  CACGTG               CAC^GTG
BbvCI         933  GCTGAGG              CCTCAGC(-5/-2)
BcgI          101  CGATCCGCGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2579  GGATAC               GTATCC(6/5)
BglII         354  AGATCT               A^GATCT
BlpI         2098  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2098  GCTGAGC              GC^T[ACGT]AGC
BsaWI        1206  TCCGGT               [AT]^CCGG[AT]
BsgI         1747  GTGCAG               GTGCAG(16/14)
BsmFI         898  GGGAC                GGGAC(10/14)
BspDI        1957  ATCGAT               AT^CGAT
BspMI         644  ACCTGC               ACCTGC(4/8)
Bst1107I      262  GTATAC               GTA^TAC
BstBI         242  TTCGAA               TT^CGAA
BstZ17I       262  GTATAC               GTA^TAC
CelII        2098  GCTGAGC              GC^T[ACGT]AGC
ClaI         1957  ATCGAT               AT^CGAT
DraI          375  TTTAAA               TTT^AAA
DraIII       1360  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     2387  GACCTAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         2083  GAAGAG               CTCTTC(1/4)
EcoRV        1828  GATATC               GAT^ATC
HgaI          307  GCGTC                GACGC(5/10)
Ksp632I      2083  GAAGAG               CTCTTC(1/4)
NspV          242  TTCGAA               TT^CGAA
PflFI         124  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         985  CCAATGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         540  CACGTG               CAC^GTG
PmlI          540  CACGTG               CAC^GTG
PstI           59  CTGCAG               CTGCA^G
SapI         2083  GAAGAGC              GCTCTTC(1/4)
SexAI        1361  ACCTGGT              A^CC[AT]GGT
SfcI           59  CTGCAG               C^T[GA][CT]AG
SfuI          242  TTCGAA               TT^CGAA
SwaI          374  ATTTAAAT             ATTT^AAAT
Tsp45I       2438  GTCAC                ^GT[GC]AC
Tth111I       124  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        985  CCAATGTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          357  TCTAGA               T^CTAGA
XcmI          588  CCAGCTGGACAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   43 enzymes) #
###################################

AccI          130  GTCTAC               GT^[AC][GT]AC
              262  GTATAC              
AccII         106  CGCG                 CG^CG
             1277  CGCG                
Asp700I      1435  GAACGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1870  GAAGAACTTC          
AspHI        1246  GAGCAC               G[AT]GC[AT]^C
             2276  GAGCAC              
BalI          851  TGGCCA               TGG^CCA
             1508  TGGCCA              
BanI           13  GGCGCC               G^G[CT][GA]CC
             2044  GGCGCC              
BbeI           13  GGCGCC               GGCGC^C
             2044  GGCGCC              
BbsI         1583  GAAGAC               GAAGAC(2/6)
             2314  GAAGAC              
BglI         1510  GCCATGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2047  GCCGAGGTGGC         
BpuAI        1583  GAAGAC               GAAGAC(2/6)
             2314  GAAGAC              
BsaAI         540  CACGTG               [CT]AC^GT[GA]
             1604  TACGTG              
BsiHKAI      1246  GAGCAC               G[AT]GC[AT]^C
             2276  GAGCAC              
BsmAI          65  GAGAC                GTCTC(1/5)
              749  GAGAC               
BsmI         1439  GCATTC               GAATGC(1/-1)
             2428  GCATTC              
BstUI         106  CGCG                 CG^CG
             1277  CGCG                
BstXI         535  CCAGACACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              616  CCAAGTGGTTGG        
DrdI          595  GACAATGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              676  GACGGCTCTGTC        
DsaI         1511  CCATGG               C^C[GA][CT]GG
             2213  CCATGG              
EcoT14I      1511  CCATGG               C^C[AT][AT]GG
             2213  CCATGG              
EcoT22I       192  ATGCAT               ATGCA^T
              892  ATGCAT              
FauI         1998  CCCGC                CCCGC(4/6)
             1699  GCGGG               
KasI           13  GGCGCC               G^GCGCC
             2044  GGCGCC              
MaeII         541  ACGT                 A^CGT
             1605  ACGT                
MfeI          482  CAATTG               C^AATTG
             1936  CAATTG              
MluNI         851  TGGCCA               TGG^CCA
             1508  TGGCCA              
MscI          851  TGGCCA               TGG^CCA
             1508  TGGCCA              
MseI          376  TTAA                 T^TAA
             1286  TTAA                
MunI          482  CAATTG               C^AATTG
             1936  CAATTG              
MvnI          106  CGCG                 CG^CG
             1277  CGCG                
NarI           13  GGCGCC               GG^CGCC
             2044  GGCGCC              
NcoI         1511  CCATGG               C^CATGG
             2213  CCATGG              
NdeI          889  CATATG               CA^TATG
             2414  CATATG              
NsiI          192  ATGCAT               ATGCA^T
              892  ATGCAT              
PpuMI         530  AGGACCC              [GA]G^G[AT]CC[CT]
             2385  AGGACCT             
SfoI           13  GGCGCC               GGC^GCC
             2044  GGCGCC              
SphI           26  GCATGC               GCATG^C
              729  GCATGC              
SspI          454  AATATT               AAT^ATT
             1110  AATATT              
StuI         1289  AGGCCT               AGG^CCT
             1637  AGGCCT              
StyI         1511  CCATGG               C^C[AT][AT]GG
             2213  CCATGG              
TaqI          243  TCGA                 T^CGA
             1958  TCGA                
Tru9I         376  TTAA                 T^TAA
             1286  TTAA                
TthHB8I       243  TCGA                 T^CGA
             1958  TCGA                
XmnI         1435  GAACGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1870  GAAGAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflIII      
AluI        AlwI        ApoI        AvaII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsiYI       BslI        Bsp1286I    
BspLU11I    BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoNI       
EcoO109I    EcoRI       EcoRII      Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       TfiI        TseI        
Tsp509I     TspRI       XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com