Restriction map for pF1KE3504
 (Linear) (Six-base) MAPSORT of: KIEE3504.seq  Check: 1714  from: 1  to: 2589



KIEE3504.orf

 With 182 enzymes: SgfI * 

                        June 20, 2019 10:05  ..

AccI GT'mk_AC

Cuts at:      0     131     263    2589 
   Size:        131     132    2326

  Fragments arranged by size: 

                2326     132     131
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194    1000    1033    1047    1618    2589 
   Size:        194     806      33      14     571     971

  Fragments arranged by size: 

                 971     806     571     194      33      14
 
AflIII A'CryG_T

Cuts at:      0     225     282     539    2561    2589 
   Size:        225      57     257    2022      28

  Fragments arranged by size: 

                2022     257     225      57      28
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2392    2589 
   Size:       2392     197
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1355    2589 
   Size:       1355    1234
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     774     806    2589 
   Size:        774      32    1783

  Fragments arranged by size: 

                1783     774      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2137    2589 
   Size:       2137     452
 
ApoI r'AATT_y

Cuts at:      0     336    1075    1315    1348    1792    2589 
   Size:        336     739     240      33     444     797

  Fragments arranged by size: 

                 797     739     444     336     240      33
 
BamHI G'GATC_C

Cuts at:      0     840    2589 
   Size:        840    1749
 
BanI G'GyrC_C

Cuts at:      0      13    2044    2589 
   Size:         13    2031     545

  Fragments arranged by size: 

                2031     545      13
 
BanII G_rGCy'C

Cuts at:      0     914    2589 
   Size:        914    1675
 
BbeI G_GCGC'C

Cuts at:      0      17    2048    2589 
   Size:         17    2031     541

  Fragments arranged by size: 

                2031     541      17
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1590    2321    2589 
   Size:       1590     731     268

  Fragments arranged by size: 

                1590     731     268
 
BbvCI CC'TCA_GC

Cuts at:      0     934    2589 
   Size:        934    1655
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      90     124    2589 
   Size:         90      34    2465

  Fragments arranged by size: 

                2465      90      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2573    2589 
   Size:       2573      16
 
BfrBI ATG'CAT

Cuts at:      0     194     894    2589 
   Size:        194     700    1695

  Fragments arranged by size: 

                1695     700     194
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1516    2053    2589 
   Size:       1516     537     536

  Fragments arranged by size: 

                1516     537     536
 
BglII A'GATC_T

Cuts at:      0     354    2589 
   Size:        354    2235
 
BlpI GC'TnA_GC

Cuts at:      0    2099    2589 
   Size:       2099     490
 
Bme1580I G_kGCm'C

Cuts at:      0      89     113     973    1683    2589 
   Size:         89      24     860     710     906

  Fragments arranged by size: 

                 906     860     710      89      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     787    1788    2589 
   Size:        787    1001     801

  Fragments arranged by size: 

                1001     801     787
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     673    2589 
   Size:        673    1916
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     757     898    1692    2162    2168    2589 
   Size:        757     141     794     470       6     421

  Fragments arranged by size: 

                 794     757     470     421     141       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     934    2589 
   Size:        934    1655
 
BsaAI yAC'GTr

Cuts at:      0     542    1606    2589 
   Size:        542    1064     983

  Fragments arranged by size: 

                1064     983     542
 
BsaHI Gr'CG_yC

Cuts at:      0      14     307    2045    2589 
   Size:         14     293    1738     544

  Fragments arranged by size: 

                1738     544     293      14
 
BsaWI w'CCGG_w

Cuts at:      0    1206    2589 
   Size:       1206    1383
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     774     804    2135    2165    2589 
   Size:        774      30    1331      30     424

  Fragments arranged by size: 

                1331     774     424      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     475    1258    1311    2589 
   Size:        475     783      53    1278

  Fragments arranged by size: 

                1278     783     475      53
 
BseYI C'CCAG_C

Cuts at:      0     587    2589 
   Size:        587    2002
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1768    2589 
   Size:       1768     821
 
BsiHKAI G_wGCw'C

Cuts at:      0    1250    2280    2589 
   Size:       1250    1030     309

  Fragments arranged by size: 

                1250    1030     309
 
BsmI GAATG_Cn'

Cuts at:      0    1439    2428    2589 
   Size:       1439     989     161

  Fragments arranged by size: 

                1439     989     161
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     113     914     973    1250    1683    2280    2589 
   Size:         89      24     801      59     277     433     597     309

  Fragments arranged by size: 

                 801     597     433     309     277      89      59      24
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     653    2589 
   Size:        653    1936
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1031    1445    1696    2137    2589 
   Size:       1031     414     251     441     452

  Fragments arranged by size: 

                1031     452     441     414     251
 
BstBI TT'CG_AA

Cuts at:      0     243    2589 
   Size:        243    2346
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     542     623    2589 
   Size:        542      81    1966

  Fragments arranged by size: 

                1966     542      81
 
BstYI r'GATC_y

Cuts at:      0     354     840    1131    1268    2288    2404    2589 
   Size:        354     486     291     137    1020     116     185

  Fragments arranged by size: 

                1020     486     354     291     185     137     116
 
BstZ17I GTA'TAC

Cuts at:      0     264    2589 
   Size:        264    2325
 
BtgI C'CryG_G

Cuts at:      0    1511    2213    2589 
   Size:       1511     702     376

  Fragments arranged by size: 

                1511     702     376
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1424    2589 
   Size:       1424    1165
 
BtsI GCAGTG_nn'

Cuts at:      0    1683    2589 
   Size:       1683     906
 
ClaI AT'CG_AT

Cuts at:      0    1958    2589 
   Size:       1958     631
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2506    2541    2589 
   Size:       2506      35      48

  Fragments arranged by size: 

                2506      48      35
 
DraI TTT'AAA

Cuts at:      0     377    2589 
   Size:        377    2212
 
DraIII CAC_nnn'GTG

Cuts at:      0    1365    2589 
   Size:       1365    1224
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     601     682    2589 
   Size:        601      81    1907

  Fragments arranged by size: 

                1907     601      81
 
EaeI y'GGCC_r

Cuts at:      0     851    1508    1982    2589 
   Size:        851     657     474     607

  Fragments arranged by size: 

                 851     657     607     474
 
EarI CTCTTCn'nnn_

Cuts at:      0    2078    2589 
   Size:       2078     511
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     757     898    1000    1033    1047    1618    1692 
   Size:        194     563     141     102      33      14     571      74

Cuts at:   1692    2162    2168    2589 
   Size:        470       6     421

  Fragments arranged by size: 

                 571     563     470     421     194     141     102      74
                  33      14       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     216    1326    2535    2589 
   Size:        216    1110    1209      54

  Fragments arranged by size: 

                1209    1110     216      54
 
EcoO109I rG'GnC_Cy

Cuts at:      0     531    2386    2540    2589 
   Size:        531    1855     154      49

  Fragments arranged by size: 

                1855     531     154      49
 
EcoRI G'AATT_C

Cuts at:      0     336    1075    1315    1348    2589 
   Size:        336     739     240      33    1241

  Fragments arranged by size: 

                1241     739     336     240      33
 
EcoRV GAT'ATC

Cuts at:      0    1830    2589 
   Size:       1830     759
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     430     720    2589 
   Size:        430     290    1869

  Fragments arranged by size: 

                1869     430     290
 
HaeII r_GCGC'y

Cuts at:      0       9      17    2048    2589 
   Size:          9       8    2031     541

  Fragments arranged by size: 

                2031     541       9       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1951    1983    2378    2410    2589 
   Size:       1951      32     395      32     179

  Fragments arranged by size: 

                1951     395     179      32      32
 
KasI G'GCGC_C

Cuts at:      0      13    2044    2589 
   Size:         13    2031     545

  Fragments arranged by size: 

                2031     545      13
 
MfeI C'AATT_G

Cuts at:      0     482    1936    2589 
   Size:        482    1454     653

  Fragments arranged by size: 

                1454     653     482
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     604    1159    2589 
   Size:        604     555    1430

  Fragments arranged by size: 

                1430     604     555
 
MscI TGG'CCA

Cuts at:      0     853    1510    2589 
   Size:        853     657    1079

  Fragments arranged by size: 

                1079     853     657
 
MslI CAynn'nnrTG

Cuts at:      0     893    1156    2212    2589 
   Size:        893     263    1056     377

  Fragments arranged by size: 

                1056     893     377     263
 
MspA1I CmG'CkG

Cuts at:      0      41     591    1031    1980    2001    2106    2589 
   Size:         41     550     440     949      21     105     483

  Fragments arranged by size: 

                 949     550     483     440     105      41      21
 
NarI GG'CG_CC

Cuts at:      0      14    2045    2589 
   Size:         14    2031     544

  Fragments arranged by size: 

                2031     544      14
 
NcoI C'CATG_G

Cuts at:      0    1511    2213    2589 
   Size:       1511     702     376

  Fragments arranged by size: 

                1511     702     376
 
NdeI CA'TA_TG

Cuts at:      0     890    2415    2589 
   Size:        890    1525     174

  Fragments arranged by size: 

                1525     890     174
 
NsiI A_TGCA'T

Cuts at:      0     196     896    2589 
   Size:        196     700    1693

  Fragments arranged by size: 

                1693     700     196
 
NspI r_CATG'y

Cuts at:      0      30      96     229     270     286     733    1284    2012 
   Size:         30      66     133      41      16     447     551     728

Cuts at:   2012    2565    2589 
   Size:        553      24

  Fragments arranged by size: 

                 728     553     551     447     133      66      41      30
                  24      16
 
PciI A'CATG_T

Cuts at:      0     225     282    2561    2589 
   Size:        225      57    2279      28

  Fragments arranged by size: 

                2279     225      57      28
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     991    2589 
   Size:        991    1598
 
PfoI T'CCnGG_A

Cuts at:      0     914     959    2589 
   Size:        914      45    1630

  Fragments arranged by size: 

                1630     914      45
 
PmlI CAC'GTG

Cuts at:      0     542    2589 
   Size:        542    2047
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     774     806    1255    1287    2589 
   Size:        774      32     449      32    1302

  Fragments arranged by size: 

                1302     774     449      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     531    2386    2589 
   Size:        531    1855     203

  Fragments arranged by size: 

                1855     531     203
 
PstI C_TGCA'G

Cuts at:      0      63    2589 
   Size:         63    2526
 
PvuII CAG'CTG

Cuts at:      0     591    1031    1980    2589 
   Size:        591     440     949     609

  Fragments arranged by size: 

                 949     609     591     440
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2078    2589 
   Size:       2078     511
 
SexAI A'CCwGG_T

Cuts at:      0    1361    2589 
   Size:       1361    1228
 
SfcI C'TryA_G

Cuts at:      0      59    2589 
   Size:         59    2530
 
SfoI GGC'GCC

Cuts at:      0      15    2046    2589 
   Size:         15    2031     543

  Fragments arranged by size: 

                2031     543      15
 
SphI G_CATG'C

Cuts at:      0      30     733    2589 
   Size:         30     703    1856

  Fragments arranged by size: 

                1856     703      30
 
SspI AAT'ATT

Cuts at:      0     456    1112    2589 
   Size:        456     656    1477

  Fragments arranged by size: 

                1477     656     456
 
StuI AGG'CCT

Cuts at:      0    1291    1639    2589 
   Size:       1291     348     950

  Fragments arranged by size: 

                1291     950     348
 
StyI C'CwwG_G

Cuts at:      0    1511    2213    2589 
   Size:       1511     702     376

  Fragments arranged by size: 

                1511     702     376
 
SwaI ATTT'AAAT

Cuts at:      0     377    2589 
   Size:        377    2212
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     601    2490    2589 
   Size:        601    1889      99

  Fragments arranged by size: 

                1889     601      99
 
TatI w'GTAC_w

Cuts at:      0    2024    2564    2589 
   Size:       2024     540      25

  Fragments arranged by size: 

                2024     540      25
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2486    2518    2589 
   Size:       2486      32      71

  Fragments arranged by size: 

                2486      71      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     127    2589 
   Size:        127    2462
 
XbaI T'CTAG_A

Cuts at:      0     357    2589 
   Size:        357    2232
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     595    2589 
   Size:        595    1994
 
XmnI GAAnn'nnTTC

Cuts at:      0    1439    1874    2589 
   Size:       1439     435     715

  Fragments arranged by size: 

                1439     715     435
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     AlfI     AloI    AlwNI     ApoI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI    HaeII    Hin4I     KasI     MfeI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NsiI     NspI     PciI    PflMI
     PfoI     PmlI     PpiI    PpuMI     PstI    PvuII     SapI    SexAI
     SfcI     SfoI     SphI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BclI    BmgBI     BmtI    BpuEI     BsaI    BsaBI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstEII   Bsu36I     EagI     EciI  EcoICRI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MluI     NaeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PmeI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SrfI     XhoI     XmaI     ZraI
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