Restriction map for pF1KE1061
 (Linear) (Six-base) MAPSORT of: KIEE1061.seq  Check: 7550  from: 1  to: 897



KIEE1061.orf

 With 182 enzymes: SgfI * 

                        November 27, 2008 10:48  ..

AclI AA'CG_TT

Cuts at:      0     463     897 
   Size:        463     434
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     815     876     897 
   Size:        815      61      21

  Fragments arranged by size: 

                 815      61      21
 
AleI CACnn'nnGTG

Cuts at:      0     154     897 
   Size:        154     743
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     116     456     897 
   Size:        116     340     441

  Fragments arranged by size: 

                 441     340     116
 
AlwNI CAG_nnn'CTG

Cuts at:      0     648     808     897 
   Size:        648     160      89

  Fragments arranged by size: 

                 648     160      89
 
ApoI r'AATT_y

Cuts at:      0     357     558     897 
   Size:        357     201     339

  Fragments arranged by size: 

                 357     339     201
 
BanII G_rGCy'C

Cuts at:      0     335     897 
   Size:        335     562
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     544     897 
   Size:        544     353
 
BbvCI CC'TCA_GC

Cuts at:      0     786     851     897 
   Size:        786      65      46

  Fragments arranged by size: 

                 786      65      46
 
BglII A'GATC_T

Cuts at:      0     117     897 
   Size:        117     780
 
BmtI G_CTAG'C

Cuts at:      0     376     460     897 
   Size:        376      84     437

  Fragments arranged by size: 

                 437     376      84
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     725     897 
   Size:         31     694     172

  Fragments arranged by size: 

                 694     172      31
 
Bpu10I CC'TnA_GC

Cuts at:      0     786     851     897 
   Size:        786      65      46

  Fragments arranged by size: 

                 786      65      46
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     396     786     840     892     897 
   Size:        396     390      54      52       5

  Fragments arranged by size: 

                 396     390      54      52       5
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     186     216     897 
   Size:        186      30     681

  Fragments arranged by size: 

                 681     186      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     191     774     897 
   Size:        191     583     123

  Fragments arranged by size: 

                 583     191     123
 
BsiHKAI G_wGCw'C

Cuts at:      0     335     897 
   Size:        335     562
 
Bsp1286I G_dGCh'C

Cuts at:      0     335     897 
   Size:        335     562
 
BspHI T'CATG_A

Cuts at:      0     632     897 
   Size:        632     265
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      41     508     897 
   Size:         41     467     389

  Fragments arranged by size: 

                 467     389      41
 
BstYI r'GATC_y

Cuts at:      0     117     488     645     708     897 
   Size:        117     371     157      63     189

  Fragments arranged by size: 

                 371     189     157     117      63
 
BtgI C'CryG_G

Cuts at:      0      34     821     897 
   Size:         34     787      76

  Fragments arranged by size: 

                 787      76      34
 
BtsI GCAGTG_nn'

Cuts at:      0     194     363     897 
   Size:        194     169     534

  Fragments arranged by size: 

                 534     194     169
 
ClaI AT'CG_AT

Cuts at:      0     531     746     897 
   Size:        531     215     151

  Fragments arranged by size: 

                 531     215     151
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     112     147     897 
   Size:        112      35     750

  Fragments arranged by size: 

                 750     112      35
 
EaeI y'GGCC_r

Cuts at:      0      71     897 
   Size:         71     826
 
EarI CTCTTCn'nnn_

Cuts at:      0     895     897 
   Size:        895       2
 
EcoICRI GAG'CTC

Cuts at:      0     333     897 
   Size:        333     564
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     725     815     876     897 
   Size:         31     694      90      61      21

  Fragments arranged by size: 

                 694      90      61      31      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     835     897 
   Size:        835      62
 
HaeII r_GCGC'y

Cuts at:      0     704     897 
   Size:        704     193
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     396     428     513     545     802     834     897 
   Size:        396      32      85      32     257      32      63

  Fragments arranged by size: 

                 396     257      85      63      32      32      32
 
MscI TGG'CCA

Cuts at:      0      73     897 
   Size:         73     824
 
MslI CAynn'nnrTG

Cuts at:      0      39     154     438     897 
   Size:         39     115     284     459

  Fragments arranged by size: 

                 459     284     115      39
 
MspA1I CmG'CkG

Cuts at:      0     855     897 
   Size:        855      42
 
NcoI C'CATG_G

Cuts at:      0      34     897 
   Size:         34     863
 
NdeI CA'TA_TG

Cuts at:      0     473     897 
   Size:        473     424
 
NheI G'CTAG_C

Cuts at:      0     372     456     897 
   Size:        372      84     441

  Fragments arranged by size: 

                 441     372      84
 
NspI r_CATG'y

Cuts at:      0     372     605     897 
   Size:        372     233     292

  Fragments arranged by size: 

                 372     292     233
 
PstI C_TGCA'G

Cuts at:      0     210     644     845     897 
   Size:        210     434     201      52

  Fragments arranged by size: 

                 434     210     201      52
 
PvuII CAG'CTG

Cuts at:      0     855     897 
   Size:        855      42
 
SacI G_AGCT'C

Cuts at:      0     335     897 
   Size:        335     562
 
SfcI C'TryA_G

Cuts at:      0      46     206     640     841     897 
   Size:         46     160     434     201      56

  Fragments arranged by size: 

                 434     201     160      56      46
 
SpeI A'CTAG_T

Cuts at:      0     151     897 
   Size:        151     746
 
SphI G_CATG'C

Cuts at:      0     372     605     897 
   Size:        372     233     292

  Fragments arranged by size: 

                 372     292     233
 
StuI AGG'CCT

Cuts at:      0      20     897 
   Size:         20     877
 
StyI C'CwwG_G

Cuts at:      0      34      74     173     880     897 
   Size:         34      40      99     707      17

  Fragments arranged by size: 

                 707      99      40      34      17
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     186     218     659     691     897 
   Size:        186      32     441      32     206

  Fragments arranged by size: 

                 441     206     186      32      32
 

 Enzymes that do cut:

     AclI     AcuI     AleI     AlfI    AlwNI     ApoI    BanII     BbsI
    BbvCI    BglII     BmtI     BpmI   Bpu10I     BsaI    BsaXI    BseRI
  BsiHKAI Bsp1286I    BspHI    BstXI    BstYI     BtgI     BtsI     ClaI
    CspCI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NspI     PstI
    PvuII     SacI     SfcI     SpeI     SphI     StuI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     DraI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI   NgoMIV     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SrfI     SspI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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