Restriction map for pF1KB7918
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
AvrII       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaWI       BseAI       BsgI        
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstXI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
Eco47III    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HinfI       HpaI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MroI        
MseI        MslI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshBI       Psp1406I    RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tth111I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AhdI          252  GACTGATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         404  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          939  CCTGAGG              CC^T[ACGT]AGG
ApaI          378  GGGCCC               GGGCC^C
AspEI         252  GACTGATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         934  GAGCTC               G[AT]GC[AT]^C
BalI          146  TGGCCA               TGG^CCA
BbeI          289  GGCGCC               GGCGC^C
BsaI          895  GAGACC               GGTCTC(1/5)
BsiHKAI       934  GAGCTC               G[AT]GC[AT]^C
BspHI         593  TCATGA               T^CATGA
BsrDI         452  GCAATG               GCAATG(2/0)
BstAPI        493  GCACGATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         774  TTCGAA               TT^CGAA
Bsu36I        939  CCTGAGG              CC^T[ACGT]AGG
DdeI          940  CTGAG                C^T[ACGT]AG
EagI          470  CGGCCG               C^GGCCG
Eam1105I      252  GACTGATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          707  GAAGAG               CTCTTC(1/4)
EclXI         470  CGGCCG               C^GGCCG
Eco52I        470  CGGCCG               C^GGCCG
Eco81I        939  CCTGAGG              CC^T[ACGT]AGG
EcoNI         837  CCTGTCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       842  CCAAGG               C^C[AT][AT]GG
KasI          289  GGCGCC               G^GCGCC
Ksp632I       707  GAAGAG               CTCTTC(1/4)
MaeII         590  ACGT                 A^CGT
MluNI         146  TGGCCA               TGG^CCA
MscI          146  TGGCCA               TGG^CCA
NarI          289  GGCGCC               GG^CGCC
NspV          774  TTCGAA               TT^CGAA
PshAI          13  GACCCGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI          638  CGATCG               CGAT^CG
RcaI          593  TCATGA               T^CATGA
SacI          934  GAGCTC               GAGCT^C
SapI          707  GAAGAGC              GCTCTTC(1/4)
SfoI          289  GGCGCC               GGC^GCC
SfuI          774  TTCGAA               TT^CGAA
SphI          887  GCATGC               GCATG^C
StuI          845  AGGCCT               AGG^CCT
StyI          842  CCAAGG               C^C[AT][AT]GG
Tsp509I       672  AATT                 ^AATT
TspRI         519  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI          246  CCAGGTGACTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII         238  CGCG                 CG^CG
              321  CGCG                
AfaI          195  GTAC                 GT^AC
              547  GTAC                
BamHI          24  GGATCC               G^GATCC
              833  GGATCC              
BanI          289  GGCGCC               G^G[CT][GA]CC
             1027  GGCACC              
BpmI          575  CTGGAG               CTGGAG(16/14)
             1009  CTGGAG              
BsiEI         470  CGGCCG               CG[GA][CT]^CG
              638  CGATCG              
BsmAI         211  GAGAC                GTCTC(1/5)
              895  GAGAC               
BstUI         238  CGCG                 CG^CG
              321  CGCG                
BstYI          24  GGATCC               [GA]^GATC[CT]
              833  GGATCC              
MboII         707  GAAGA                GAAGA(8/7)
              963  GAAGA               
MflI           24  GGATCC               [GA]^GATC[CT]
              833  GGATCC              
MvnI          238  CGCG                 CG^CG
              321  CGCG                
NspI            5  ACATGC               [GA]CATG^[CT]
              887  GCATGC              
PpuMI          72  GGGACCC              [GA]G^G[AT]CC[CT]
              405  AGGTCCT             
RsaI          195  GTAC                 GT^AC
              547  GTAC                
SmaI           15  CCCGGG               CCC^GGG
              375  CCCGGG              
XhoII          24  GGATCC               [GA]^GATC[CT]
              833  GGATCC              
XmaCI          15  CCCGGG               C^CCGGG
              375  CCCGGG              
XmaI           15  CCCGGG               C^CCGGG
              375  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        AvaI        
AvaII       BanII       BbvI        BcnI        BmyI        
BsaHI       BsaJI       BseRI       BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BsrBI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DpnII       DraII       EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        MaeIII      
MboI        MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PstI        PvuII       Sau3AI      Sau96I      ScrFI       
SfcI        TaqI        TseI        Tsp45I      TthHB8I     
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