Restriction map for pF1KE0297
 (Linear) (Six-base) MAPSORT of: KIEE0297.seq  Check: 3853  from: 1  to: 4758



KIEE0297.orf

 With 182 enzymes: SgfI * 

                        October 29, 2008 11:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1577    3240    3620    4297    4758 
   Size:       1577    1663     380     677     461

  Fragments arranged by size: 

                1663    1577     677     461     380
 
AatII G_ACGT'C

Cuts at:      0      10    4758 
   Size:         10    4748
 
AccI GT'mk_AC

Cuts at:      0    3461    4758 
   Size:       3461    1297
 
Acc65I G'GTAC_C

Cuts at:      0     215    4758 
   Size:        215    4543
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1579    2295    4758 
   Size:       1579     716    2463

  Fragments arranged by size: 

                2463    1579     716
 
AfeI AGC'GCT

Cuts at:      0    2956    4758 
   Size:       2956    1802
 
AflII C'TTAA_G

Cuts at:      0     472    4758 
   Size:        472    4286
 
AflIII A'CryG_T

Cuts at:      0     641    2582    4355    4758 
   Size:        641    1941    1773     403

  Fragments arranged by size: 

                1941    1773     641     403
 
AgeI A'CCGG_T

Cuts at:      0    2180    4758 
   Size:       2180    2578
 
AleI CACnn'nnGTG

Cuts at:      0    3221    4758 
   Size:       3221    1537
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1822    1840    2752    3548    3832    4758 
   Size:       1822      18     912     796     284     926

  Fragments arranged by size: 

                1822     926     912     796     284      18
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3893    3925    4723    4755    4758 
   Size:       3893      32     798      32       3

  Fragments arranged by size: 

                3893     798      32      32       3
 
AlwNI CAG_nnn'CTG

Cuts at:      0      22     897    1037    2689    3672    4176    4243    4512 
   Size:         22     875     140    1652     983     504      67     269

Cuts at:   4512    4758 
   Size:        246

  Fragments arranged by size: 

                1652     983     875     504     269     246     140      67
                  22
 
ApaI G_GGCC'C

Cuts at:      0     257    1980    2063    2336    3146    3355    3786    4317 
   Size:        257    1723      83     273     810     209     431     531

Cuts at:   4317    4758 
   Size:        441

  Fragments arranged by size: 

                1723     810     531     441     431     273     257     209
                  83
 
ApaLI G'TGCA_C

Cuts at:      0     247     397    1474    1512    1807    4758 
   Size:        247     150    1077      38     295    2951

  Fragments arranged by size: 

                2951    1077     295     247     150      38
 
AvaI C'yCGr_G

Cuts at:      0      81    1972    2062    2283    2595    2767    3036    3145 
   Size:         81    1891      90     221     312     172     269     109

Cuts at:   3145    3303    3549    4123    4387    4563    4575    4758 
   Size:        158     246     574     264     176      12     183

  Fragments arranged by size: 

                1891     574     312     269     264     246     221     183
                 176     172     158     109      90      81      12
 
BamHI G'GATC_C

Cuts at:      0      53    4758 
   Size:         53    4705
 
BanI G'GyrC_C

Cuts at:      0     215     508     835    2524    3064    3241    3396    3454 
   Size:        215     293     327    1689     540     177     155      58

Cuts at:   3454    3842    3864    4278    4758 
   Size:        388      22     414     480

  Fragments arranged by size: 

                1689     540     480     414     388     327     293     215
                 177     155      58      22
 
BanII G_rGCy'C

Cuts at:      0     257    1951    1980    2003    2063    2336    2551    3146 
   Size:        257    1694      29      23      60     273     215     595

Cuts at:   3146    3355    3451    3633    3727    3766    3786    4061    4130 
   Size:        209      96     182      94      39      20     275      69

Cuts at:   4130    4317    4591    4708    4758 
   Size:        187     274     117      50

  Fragments arranged by size: 

                1694     595     275     274     273     257     215     209
                 187     182     117      96      94      69      60      50
                  39      29      23      20
 
BbeI G_GCGC'C

Cuts at:      0    2528    3068    3400    4758 
   Size:       2528     540     332    1358

  Fragments arranged by size: 

                2528    1358     540     332
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1331    1567    4758 
   Size:       1331     236    3191

  Fragments arranged by size: 

                3191    1331     236
 
BbvCI CC'TCA_GC

Cuts at:      0      15     265     442     841    1289    2118    3848    4758 
   Size:         15     250     177     399     448     829    1730     910

  Fragments arranged by size: 

                1730     910     829     448     399     250     177      15
            
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     177     211    1645    1679    4758 
   Size:        177      34    1434      34    3079

  Fragments arranged by size: 

                3079    1434     177      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3266    4758 
   Size:       3266    1492
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2340    2902    3121    3386    3587    4004    4046    4139 
   Size:       2340     562     219     265     201     417      42      93

Cuts at:   4139    4758 
   Size:        619

  Fragments arranged by size: 

                2340     619     562     417     265     219     201      93
                  42
 
BglII A'GATC_T

Cuts at:      0    1257    4758 
   Size:       1257    3501
 
BlpI GC'TnA_GC

Cuts at:      0     798    1021    2395    3760    4758 
   Size:        798     223    1374    1365     998

  Fragments arranged by size: 

                1374    1365     998     798     223
 
Bme1580I G_kGCm'C

Cuts at:      0     251     257     401     840    1218    1478    1516    1811 
   Size:        251       6     144     439     378     260      38     295

Cuts at:   1811    1980    2022    2063    2336    3146    3244    3355    3786 
   Size:        169      42      41     273     810      98     111     431

Cuts at:   3786    3845    4250    4317    4758 
   Size:         59     405      67     441

  Fragments arranged by size: 

                 810     441     439     431     405     378     295     273
                 260     251     169     144     111      98      67      59
                  42      41      38       6
 
BmgBI CAC'GTC

Cuts at:      0     642    1782    1806    1926    3195    4758 
   Size:        642    1140      24     120    1269    1563

  Fragments arranged by size: 

                1563    1269    1140     642     120      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1329    1974    2812    3943    4758 
   Size:       1329     645     838    1131     815

  Fragments arranged by size: 

                1329    1131     838     815     645
 
BmtI G_CTAG'C

Cuts at:      0    3581    4758 
   Size:       3581    1177
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1545    3465    4758 
   Size:       1545    1920    1293

  Fragments arranged by size: 

                1920    1545    1293
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     488     639    1570    2134    2475    2487    2743 
   Size:         79     409     151     931     564     341      12     256

Cuts at:   2743    2867    3387    3950    4399    4534    4758 
   Size:        124     520     563     449     135     224

  Fragments arranged by size: 

                 931     564     563     520     449     409     341     256
                 224     151     135     124      79      12
 
Bpu10I CC'TnA_GC

Cuts at:      0      15     265     442     679     841    1289    2118    3848 
   Size:         15     250     177     237     162     448     829    1730

Cuts at:   3848    4758 
   Size:        910

  Fragments arranged by size: 

                1730     910     829     448     250     237     177     162
                  15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1234    2179    2194    2203    4758 
   Size:       1234     945      15       9    2555

  Fragments arranged by size: 

                2555    1234     945      15       9
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1300    2069    4758 
   Size:       1300     769    2689

  Fragments arranged by size: 

                2689    1300     769
 
BsaAI yAC'GTr

Cuts at:      0     363    4159    4758 
   Size:        363    3796     599

  Fragments arranged by size: 

                3796     599     363
 
BsaBI GATnn'nnATC

Cuts at:      0    4556    4758 
   Size:       4556     202
 
BsaHI Gr'CG_yC

Cuts at:      0       7     670     719    2525    3065    3397    4758 
   Size:          7     663      49    1806     540     332    1361

  Fragments arranged by size: 

                1806    1361     663     540     332      49       7
 
BsaWI w'CCGG_w

Cuts at:      0    1742    2180    4758 
   Size:       1742     438    2578

  Fragments arranged by size: 

                2578    1742     438
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     599     629    1396    1426    2533    2557    2563    2587 
   Size:        599      30     767      30    1107      24       6      24

Cuts at:   2587    4594    4624    4690    4720    4723    4753    4758 
   Size:       2007      30      66      30       3      30       5

  Fragments arranged by size: 

                2007    1107     767     599      66      30      30      30
                  30      30      24      24       6       5       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1997    2457    4588    4611    4744    4758 
   Size:       1997     460    2131      23     133      14

  Fragments arranged by size: 

                2131    1997     460     133      23      14
 
BseYI C'CCAG_C

Cuts at:      0     846     974    1016    1732    3298    3369    3755    3766 
   Size:        846     128      42     716    1566      71     386      11

Cuts at:   3766    4021    4129    4286    4412    4454    4505    4591    4758 
   Size:        255     108     157     126      42      51      86     167

  Fragments arranged by size: 

                1566     846     716     386     255     167     157     128
                 126     108      86      71      51      42      42      11
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1314    1413    1695    1906    2634    2971    3220    3295 
   Size:       1314      99     282     211     728     337     249      75

Cuts at:   3295    3451    3541    4126    4186    4758 
   Size:        156      90     585      60     572

  Fragments arranged by size: 

                1314     728     585     572     337     282     249     211
                 156      99      90      75      60
 
BsiEI CG_ry'CG

Cuts at:      0    1082    1156    2532    3082    4758 
   Size:       1082      74    1376     550    1676

  Fragments arranged by size: 

                1676    1376    1082     550      74
 
BsiHKAI G_wGCw'C

Cuts at:      0     251     383     401     470    1385    1478    1516    1610 
   Size:        251     132      18      69     915      93      38      94

Cuts at:   1610    1811    1882    1951    2551    3043    3451    4584    4708 
   Size:        201      71      69     600     492     408    1133     124

Cuts at:   4708    4758 
   Size:         50

  Fragments arranged by size: 

                1133     915     600     492     408     251     201     132
                 124      94      93      71      69      69      50      38
                  18
 
BsiWI C'GTAC_G

Cuts at:      0     359    4758 
   Size:        359    4399
 
Bsp1286I G_dGCh'C

Cuts at:      0     251     257     383     401     470     840    1218    1385 
   Size:        251       6     126      18      69     370     378     167

Cuts at:   1385    1478    1516    1610    1811    1882    1951    1980    2003 
   Size:         93      38      94     201      71      69      29      23

Cuts at:   2003    2022    2063    2336    2551    3043    3146    3244    3355 
   Size:         19      41     273     215     492     103      98     111

Cuts at:   3355    3451    3633    3727    3766    3786    3845    4061    4130 
   Size:         96     182      94      39      20      59     216      69

Cuts at:   4130    4250    4317    4584    4591    4708    4758 
   Size:        120      67     267       7     117      50

  Fragments arranged by size: 

                 492     378     370     273     267     251     216     215
                 201     182     167     126     120     117     111     103
                  98      96      94      94      93      71      69      69
                  69      67      59      50      41      39      38      29
                  23      20      19      18       7       6
 
BspEI T'CCGG_A

Cuts at:      0    1742    4758 
   Size:       1742    3016
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     584     740    1577    3240    3620    4297    4758 
   Size:        584     156     837    1663     380     677     461

  Fragments arranged by size: 

                1663     837     677     584     461     380     156
 
BsrBI CCG'CTC

Cuts at:      0     590    2465    2501    4758 
   Size:        590    1875      36    2257

  Fragments arranged by size: 

                2257    1875     590      36
 
BsrDI GCAATG_nn'

Cuts at:      0    1806    4758 
   Size:       1806    2952
 
BsrFI r'CCGG_y

Cuts at:      0    1155    1852    2180    2527    2646    2785    4758 
   Size:       1155     697     328     347     119     139    1973

  Fragments arranged by size: 

                1973    1155     697     347     328     139     119
 
BssSI C'ACGA_G

Cuts at:      0     544    1609    2875    4758 
   Size:        544    1065    1266    1883

  Fragments arranged by size: 

                1883    1266    1065     544
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     162    1362    1464    3672    4758 
   Size:        162    1200     102    2208    1086

  Fragments arranged by size: 

                2208    1200    1086     162     102
 
BstEII G'GTnAC_C

Cuts at:      0    2385    4758 
   Size:       2385    2373
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     969    1192    3703    3882    4497    4758 
   Size:        969     223    2511     179     615     261

  Fragments arranged by size: 

                2511     969     615     261     223     179
 
BstYI r'GATC_y

Cuts at:      0      53    1257    1700    4349    4758 
   Size:         53    1204     443    2649     409

  Fragments arranged by size: 

                2649    1204     443     409      53
 
Bsu36I CC'TnA_GG

Cuts at:      0    1142    3500    4758 
   Size:       1142    2358    1258

  Fragments arranged by size: 

                2358    1258    1142
 
BtgI C'CryG_G

Cuts at:      0     240     635     661     878     983    1389    1764    1907 
   Size:        240     395      26     217     105     406     375     143

Cuts at:   1907    2336    2356    2576    2809    2841    2944    3060    3116 
   Size:        429      20     220     233      32     103     116      56

Cuts at:   3116    3260    4149    4205    4758 
   Size:        144     889      56     553

  Fragments arranged by size: 

                 889     553     429     406     395     375     240     233
                 220     217     144     143     116     105     103      56
                  56      32      26      20
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3121    4273    4758 
   Size:       3121    1152     485

  Fragments arranged by size: 

                3121    1152     485
 
BtsI GCAGTG_nn'

Cuts at:      0      20    1077    2022    2325    3439    4758 
   Size:         20    1057     945     303    1114    1319

  Fragments arranged by size: 

                1319    1114    1057     945     303      20
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2016    2051    4758 
   Size:       2016      35    2707

  Fragments arranged by size: 

                2707    2016      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     246    2976    4758 
   Size:        246    2730    1782

  Fragments arranged by size: 

                2730    1782     246
 
EaeI y'GGCC_r

Cuts at:      0     803    1079    1153    2529    2838    2844    3079    3315 
   Size:        803     276      74    1376     309       6     235     236

Cuts at:   3315    3470    3540    3741    4140    4344    4758 
   Size:        155      70     201     399     204     414

  Fragments arranged by size: 

                1376     803     414     399     309     276     236     235
                 204     201     155      74      70       6
 
EagI C'GGCC_G

Cuts at:      0    1079    1153    2529    3079    4758 
   Size:       1079      74    1376     550    1679

  Fragments arranged by size: 

                1679    1376    1079     550      74
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2906    3010    3348    4758 
   Size:       2906     104     338    1410

  Fragments arranged by size: 

                2906    1410     338     104
 
EcoICRI GAG'CTC

Cuts at:      0    1949    2549    3449    4706    4758 
   Size:       1949     600     900    1257      52

  Fragments arranged by size: 

                1949    1257     900     600      52
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     488     639    1570    1579    2134    2295    2475 
   Size:         79     409     151     931       9     555     161     180

Cuts at:   2475    2487    2743    2867    3387    3950    4399    4534    4758 
   Size:         12     256     124     520     563     449     135     224

  Fragments arranged by size: 

                 931     563     555     520     449     409     256     224
                 180     161     151     135     124      79      12       9
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     503    1228    4185    4726    4758 
   Size:        503     725    2957     541      32

  Fragments arranged by size: 

                2957     725     541     503      32
 
EcoO109I rG'GnC_Cy

Cuts at:      0     254     438     573    1146    1976    2059    3142    3352 
   Size:        254     184     135     573     830      83    1083     210

Cuts at:   3352    3588    3782    3783    3909    4017    4313    4314    4518 
   Size:        236     194       1     126     108     296       1     204

Cuts at:   4518    4758 
   Size:        240

  Fragments arranged by size: 

                1083     830     573     296     254     240     236     210
                 204     194     184     135     126     108      83       1
                   1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3552    4758 
   Size:       3552    1206
 
FseI GG_CCGG'CC

Cuts at:      0    1159    4758 
   Size:       1159    3599
 
FspI TGC'GCA

Cuts at:      0    2158    4758 
   Size:       2158    2600
 
HaeII r_GCGC'y

Cuts at:      0     599     697    1174    2415    2528    2595    2755    2958 
   Size:        599      98     477    1241     113      67     160     203

Cuts at:   2958    3068    3230    3400    4758 
   Size:        110     162     170    1358

  Fragments arranged by size: 

                1358    1241     599     477     203     170     162     160
                 113     110      98      67
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1720    1752    2215    2247    2557    2589    2920    2952 
   Size:       1720      32     463      32     310      32     331      32

Cuts at:   2952    3873    3905    4690    4722    4758 
   Size:        921      32     785      32      36

  Fragments arranged by size: 

                1720     921     785     463     331     310      36      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1098    4479    4758 
   Size:       1098    3381     279

  Fragments arranged by size: 

                3381    1098     279
 
KasI G'GCGC_C

Cuts at:      0    2524    3064    3396    4758 
   Size:       2524     540     332    1362

  Fragments arranged by size: 

                2524    1362     540     332
 
KpnI G_GTAC'C

Cuts at:      0     219    4758 
   Size:        219    4539
 
MluI A'CGCG_T

Cuts at:      0    2582    4758 
   Size:       2582    2176
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1658    1700    2345    3368    4758 
   Size:       1658      42     645    1023    1390

  Fragments arranged by size: 

                1658    1390    1023     645      42
 
MscI TGG'CCA

Cuts at:      0    2840    3472    3542    3743    4758 
   Size:       2840     632      70     201    1015

  Fragments arranged by size: 

                2840    1015     632     201      70
 
MslI CAynn'nnrTG

Cuts at:      0    1209    1468    1599    3221    3427    3880    4758 
   Size:       1209     259     131    1622     206     453     878

  Fragments arranged by size: 

                1622    1209     878     453     259     206     131
 
MspA1I CmG'CkG

Cuts at:      0      67     118     130     269     451     663     738     802 
   Size:         67      51      12     139     182     212      75      64

Cuts at:    802     809     832    1239    1359    1418    1824    2140    2154 
   Size:          7      23     407     120      59     406     316      14

Cuts at:   2154    2338    2358    2403    2554    2618    2712    2811    2946 
   Size:        184      20      45     151      64      94      99     135

Cuts at:   2946    2991    3062    3626    3672    3759    4176    4758 
   Size:         45      71     564      46      87     417     582

  Fragments arranged by size: 

                 582     564     417     407     406     316     212     184
                 182     151     139     135     120      99      94      87
                  75      71      67      64      64      59      51      46
                  45      45      23      20      14      12       7
 
NaeI GCC'GGC

Cuts at:      0    1157    1854    2529    2648    2787    4758 
   Size:       1157     697     675     119     139    1971

  Fragments arranged by size: 

                1971    1157     697     675     139     119
 
NarI GG'CG_CC

Cuts at:      0    2525    3065    3397    4758 
   Size:       2525     540     332    1361

  Fragments arranged by size: 

                2525    1361     540     332
 
NcoI C'CATG_G

Cuts at:      0     983    4205    4758 
   Size:        983    3222     553

  Fragments arranged by size: 

                3222     983     553
 
NdeI CA'TA_TG

Cuts at:      0    1596    4758 
   Size:       1596    3162
 
NgoMIV G'CCGG_C

Cuts at:      0    1155    1852    2527    2646    2785    4758 
   Size:       1155     697     675     119     139    1973

  Fragments arranged by size: 

                1973    1155     697     675     139     119
 
NheI G'CTAG_C

Cuts at:      0    3577    4758 
   Size:       3577    1181
 
NspI r_CATG'y

Cuts at:      0     198    1467    1482    1753    3426    3666    3819    4332 
   Size:        198    1269      15     271    1673     240     153     513

Cuts at:   4332    4359    4758 
   Size:         27     399

  Fragments arranged by size: 

                1673    1269     513     399     271     240     198     153
                  27      15
 
PasI CC'CwG_GG

Cuts at:      0     328    2104    2520    3583    3639    3650    4758 
   Size:        328    1776     416    1063      56      11    1108

  Fragments arranged by size: 

                1776    1108    1063     416     328      56      11
 
PciI A'CATG_T

Cuts at:      0    4355    4758 
   Size:       4355     403
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1908    3981    4206    4512    4758 
   Size:       1908    2073     225     306     246

  Fragments arranged by size: 

                2073    1908     306     246     225
 
PfoI T'CCnGG_A

Cuts at:      0      56    2300    4511    4758 
   Size:         56    2244    2211     247

  Fragments arranged by size: 

                2244    2211     247      56
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4723    4755    4758 
   Size:       4723      32       3

  Fragments arranged by size: 

                4723      32       3
 
PpuMI rG'GwC_Cy

Cuts at:      0     573    3909    4017    4758 
   Size:        573    3336     108     741

  Fragments arranged by size: 

                3336     741     573     108
 
PspOMI G'GGCC_C

Cuts at:      0     253    1976    2059    2332    3142    3351    3782    4313 
   Size:        253    1723      83     273     810     209     431     531

Cuts at:   4313    4758 
   Size:        445

  Fragments arranged by size: 

                1723     810     531     445     431     273     253     209
                  83
 
PspXI vC'TCGA_Gb

Cuts at:      0    2595    4123    4563    4758 
   Size:       2595    1528     440     195

  Fragments arranged by size: 

                2595    1528     440     195
 
PstI C_TGCA'G

Cuts at:      0     117     432     737    1993    2408    3029    3617    3811 
   Size:        117     315     305    1256     415     621     588     194

Cuts at:   3811    4758 
   Size:        947

  Fragments arranged by size: 

                1256     947     621     588     415     315     305     194
                 117
 
PvuII CAG'CTG

Cuts at:      0    1239    1359    2154    2403    3672    3759    4176    4758 
   Size:       1239     120     795     249    1269      87     417     582

  Fragments arranged by size: 

                1269    1239     795     582     417     249     120      87
            
 
RsrII CG'GwC_CG

Cuts at:      0    1644    4758 
   Size:       1644    3114
 
SacI G_AGCT'C

Cuts at:      0    1951    2551    3451    4708    4758 
   Size:       1951     600     900    1257      50

  Fragments arranged by size: 

                1951    1257     900     600      50
 
SacII CC_GC'GG

Cuts at:      0     664    2339    2359    2812    2947    3063    4758 
   Size:        664    1675      20     453     135     116    1695

  Fragments arranged by size: 

                1695    1675     664     453     135     116      20
 
SanDI GG'GwC_CC

Cuts at:      0    3909    4758 
   Size:       3909     849
 
SexAI A'CCwGG_T

Cuts at:      0    3176    4013    4481    4758 
   Size:       3176     837     468     277

  Fragments arranged by size: 

                3176     837     468     277
 
SfcI C'TryA_G

Cuts at:      0     113     428     733    1989    2404    3025    3555    3571 
   Size:        113     315     305    1256     415     621     530      16

Cuts at:   3571    3613    3807    4085    4758 
   Size:         42     194     278     673

  Fragments arranged by size: 

                1256     673     621     530     415     315     305     278
                 194     113      42      16
 
SfoI GGC'GCC

Cuts at:      0    2526    3066    3398    4758 
   Size:       2526     540     332    1360

  Fragments arranged by size: 

                2526    1360     540     332
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2646    4758 
   Size:       2646    2112
 
SmaI CCC'GGG

Cuts at:      0      83    1974    2064    2285    3305    3551    4758 
   Size:         83    1891      90     221    1020     246    1207

  Fragments arranged by size: 

                1891    1207    1020     246     221      90      83
 
SmlI C'TyrA_G

Cuts at:      0     472    1249    2194    2209    2218    2595    4123    4563 
   Size:        472     777     945      15       9     377    1528     440

Cuts at:   4563    4758 
   Size:        195

  Fragments arranged by size: 

                1528     945     777     472     440     377     195      15
                   9
 
SphI G_CATG'C

Cuts at:      0    4332    4758 
   Size:       4332     426
 
StuI AGG'CCT

Cuts at:      0      94    1426    1537    2512    3017    3569    4376    4432 
   Size:         94    1332     111     975     505     552     807      56

Cuts at:   4432    4758 
   Size:        326

  Fragments arranged by size: 

                1332     975     807     552     505     326     111      94
                  56
 
StyI C'CwwG_G

Cuts at:      0     141     983    1334    4205    4250    4758 
   Size:        141     842     351    2871      45     508

  Fragments arranged by size: 

                2871     842     508     351     141      45
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     771     975    1030    1150    1199    1363    1684    1957 
   Size:        771     204      55     120      49     164     321     273

Cuts at:   1957    2983    3962    4758 
   Size:       1026     979     796

  Fragments arranged by size: 

                1026     979     796     771     321     273     204     164
                 120      55      49
 
TatI w'GTAC_w

Cuts at:      0    1460    2657    3203    4758 
   Size:       1460    1197     546    1555

  Fragments arranged by size: 

                1555    1460    1197     546
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     599     631     932     950     964     982    1396    1428 
   Size:        599      32     301      18      14      18     414      32

Cuts at:   1428    1558    1590    4594    4626    4758 
   Size:        130      32    3004      32     132

  Fragments arranged by size: 

                3004     599     414     301     132     130      32      32
                  32      32      18      18      14
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     765     876    1905    3228    3388    3736    3901    3921 
   Size:        765     111    1029    1323     160     348     165      20

Cuts at:   3921    4257    4758 
   Size:        336     501

  Fragments arranged by size: 

                1323    1029     765     501     348     336     165     160
                 111      20
 
XhoI C'TCGA_G

Cuts at:      0    2595    4123    4563    4758 
   Size:       2595    1528     440     195

  Fragments arranged by size: 

                2595    1528     440     195
 
XmaI C'CCGG_G

Cuts at:      0      81    1972    2062    2283    3303    3549    4758 
   Size:         81    1891      90     221    1020     246    1209

  Fragments arranged by size: 

                1891    1209    1020     246     221      90      81
 
XmnI GAAnn'nnTTC

Cuts at:      0    1447    4758 
   Size:       1447    3311
 
ZraI GAC'GTC

Cuts at:      0       8    4758 
   Size:          8    4750
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII
     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FseI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NspI     PasI     PciI    PflMI     PfoI     PpiI
    PpuMI   PspOMI    PspXI     PstI    PvuII    RsrII     SacI    SacII
    SanDI    SexAI     SfcI     SfoI    SgrAI     SmaI     SmlI     SphI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AclI     AhdI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BclI    BfrBI     BsmI    BsmBI    BspHI    BsrGI   BssHII    BstBI
  BstZ17I     ClaI     DraI     DrdI     EarI    EcoRI    EcoRV    FspAI
  HindIII     HpaI     MfeI     NotI     NruI     NsiI     PacI     PmeI
     PmlI    PshAI     PsiI     PsrI     PvuI     SalI     SapI     SbfI
     ScaI     SfiI     SgfI    SnaBI     SpeI     SrfI     SspI     SwaI
  Tth111I     XbaI
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