Restriction map for pF1KB6017
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AgeI        AhdI        ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BcgI        BciVI       BclI        BfrI        
BlnI        BsaBI       BseAI       BsiWI       BspEI       
BspHI       BspLU11I    BsrBI       BssHII      DraI        
Eam1105I    EarI        EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
Ksp632I     KspI        MamI        MfeI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        SacII       SalI        SapI        SbfI        
ScaI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        SwaI        TfiI        Tsp509I     
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I        779  GGTACC               G^GTACC
AccI         1435  GTATAC               GT^[AC][GT]AC
AflIII       2423  ACGTGT               A^C[GA][CT]GT
Alw44I       1444  GTGCAC               G^TGCAC
AocI          806  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       397  AGCGCT               AGC^GCT
ApaI         1364  GGGCCC               GGGCC^C
ApaLI        1444  GTGCAC               G^TGCAC
Asp718I       779  GGTACC               G^GTACC
BamHI        1070  GGATCC               G^GATCC
BbeI          431  GGCGCC               GGCGC^C
BbvCI         928  GCTGAGG              CCTCAGC(-5/-2)
BglII         572  AGATCT               A^GATCT
BsmBI         357  CGTCTC               CGTCTC(1/5)
BsmI         1383  GAATGC               GAATGC(1/-1)
Bsp1407I     2057  TGTACA               T^GTACA
BspMI        2120  ACCTGC               ACCTGC(4/8)
BsrDI        1893  CATTGC               GCAATG(2/0)
BsrGI        2057  TGTACA               T^GTACA
Bst1107I     1435  GTATAC               GTA^TAC
BstAPI       2337  GCACCGCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1456  TTCGAA               TT^CGAA
BstEII       1146  GGTCACC              G^GT[ACGT]ACC
BstPI        1146  GGTCACC              G^GT[ACGT]ACC
BstZ17I      1435  GTATAC               GTA^TAC
Bsu36I        806  CCTCAGG              CC^T[ACGT]AGG
CpoI         1408  CGGACCG              CG^G[AT]CCG
DraIII       1498  CACGACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1948  GACTTAAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1388  CGGCCG               C^GGCCG
EclXI        1388  CGGCCG               C^GGCCG
Eco47III      397  AGCGCT               AGC^GCT
Eco52I       1388  CGGCCG               C^GGCCG
Eco81I        806  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      1146  GGTCACC              G^GT[ACGT]ACC
HincII       1957  GTCAAC               GT[CT]^[GA]AC
HindII       1957  GTCAAC               GT[CT]^[GA]AC
KasI          431  GGCGCC               G^GCGCC
KpnI          779  GGTACC               GGTAC^C
MseI         1951  TTAA                 T^TAA
NarI          431  GGCGCC               GG^CGCC
NspI         2342  GCATGC               [GA]CATG^[CT]
NspV         1456  TTCGAA               TT^CGAA
PflMI        2186  CCATGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        2103  GACCAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        1408  CGGACCG              CG^G[AT]CCG
SfiI          804  GGCCTCAGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          431  GGCGCC               GGC^GCC
SfuI         1456  TTCGAA               TT^CGAA
SphI         2342  GCATGC               GCATG^C
SspBI        2057  TGTACA               T^GTACA
Tru9I        1951  TTAA                 T^TAA
Van91I       2186  CCATGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwI         1071  GATCC                GGATC(4/5)
             1070  GGATC               
AlwNI         188  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              994  CAGAGGCTG           
BalI         1346  TGGCCA               TGG^CCA
             1877  TGGCCA              
BbrPI        1033  CACGTG               CAC^GTG
             2443  CACGTG              
BfaI          622  CTAG                 C^TAG
             1465  CTAG                
BlpI          351  GCTGAGC              GC^T[ACGT]AGC
             2364  GCTGAGC             
Bpu1102I      351  GCTGAGC              GC^T[ACGT]AGC
             2364  GCTGAGC             
BsaI         1849  GAGACC               GGTCTC(1/5)
               40  GGTCTC              
BsaWI        1067  ACCGGA               [AT]^CCGG[AT]
             2153  ACCGGA              
BsiEI        1065  CGACCG               CG[GA][CT]^CG
             1388  CGGCCG              
BspDI         634  ATCGAT               AT^CGAT
              706  ATCGAT              
BssSI          32  CTCGTG               CACGAG(-5/-1)
              313  CTCGTG              
BstXI        1254  CCAGCTCCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2105  CCAATGTCCTGG        
BstYI         572  AGATCT               [GA]^GATC[CT]
             1070  GGATCC              
CelII         351  GCTGAGC              GC^T[ACGT]AGC
             2364  GCTGAGC             
ClaI          634  ATCGAT               AT^CGAT
              706  ATCGAT              
MaeI          622  CTAG                 C^TAG
             1465  CTAG                
MflI          572  AGATCT               [GA]^GATC[CT]
             1070  GGATCC              
MluNI        1346  TGGCCA               TGG^CCA
             1877  TGGCCA              
MscI         1346  TGGCCA               TGG^CCA
             1877  TGGCCA              
PmaCI        1033  CACGTG               CAC^GTG
             2443  CACGTG              
PmlI         1033  CACGTG               CAC^GTG
             2443  CACGTG              
SacI         1687  GAGCTC               GAGCT^C
             2311  GAGCTC              
SexAI         452  ACCTGGT              A^CC[AT]GGT
              983  ACCTGGT             
SmlI           69  CTTGAG               C^T[CT][GA]AG
             2314  CTCAAG              
XhoII         572  AGATCT               [GA]^GATC[CT]
             1070  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaAI       BsaHI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StuI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XcmI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com