Restriction map for pF1KB6016
##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       AccI        AgeI        AhdI        Aor51HI     
ApaI        AscI        AseI        AsnI        AspEI       
AspI        AviII       AvrII       BbvCI       BlnI        
BlpI        Bpu1102I    BsmBI       BsmI        Bsp1407I    
BspDI       BspLU11I    BspMI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraIII      DrdI        Eam1105I    Eco47III    
EcoO65I     FseI        FspI        HgaI        HindIII     
HpaI        MfeI        MluI        MunI        NheI        
NotI        NruI        NspV        PacI        PflFI       
PinAI       PmeI        PshAI       PshBI       PvuI        
RsrII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       StuI        
SwaI        Tth111I     XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

Acc65I        872  GGTACC               G^GTACC
AccIII       1993  TCCGGA               T^CCGGA
AclI          548  AACGTT               AA^CGTT
AcyI           90  GGCGCC               G[GA]^CG[CT]C
AflII        2785  CTTAAG               C^TTAAG
AflIII       2242  ACGTGT               A^C[GA][CT]GT
Alw44I       2004  GTGCAC               G^TGCAC
AocI         2452  CCTCAGG              CC^T[ACGT]AGG
ApaLI        2004  GTGCAC               G^TGCAC
Asp718I       872  GGTACC               G^GTACC
BbeI           90  GGCGCC               GGCGC^C
BbrPI        2241  CACGTG               CAC^GTG
BciVI        2374  GGATAC               GTATCC(6/5)
BclI          674  TGATCA               T^GATCA
BfrI         2785  CTTAAG               C^TTAAG
BglII        1173  AGATCT               A^GATCT
BsaAI        2241  CACGTG               [CT]AC^GT[GA]
BsaBI        1720  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          90  GGCGCC               G[GA]^CG[CT]C
BsaI         2685  GAGACC               GGTCTC(1/5)
BsaWI        1993  TCCGGA               [AT]^CCGG[AT]
BseAI        1993  TCCGGA               T^CCGGA
BseRI        1426  GAGGAG               GAGGAG(10/8)
BsiEI          78  CGGCCG               CG[GA][CT]^CG
BsiWI         134  CGTACG               C^GTACG
BsmAI        2685  GAGAC                GTCTC(1/5)
BspEI        1993  TCCGGA               T^CCGGA
BsrBI           8  GAGCGG               CCGCTC(-3/-3)
BstAPI       2267  GCACAGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       2452  CCTCAGG              CC^T[ACGT]AGG
CfoI           91  GCGC                 GCG^C
DraI         1314  TTTAAA               TTT^AAA
EagI           78  CGGCCG               C^GGCCG
EclXI          78  CGGCCG               C^GGCCG
Eco52I         78  CGGCCG               C^GGCCG
Eco81I       2452  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1281  CCTAGTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          674  TGATCA               T^GATCA
HaeII          90  GGCGCC               [GA]GCGC^[CT]
HhaI           91  GCGC                 GCG^C
Hin1I          90  GGCGCC               G[GA]^CG[CT]C
HinP1I         91  GCGC                 G^CGC
HincII        223  GTTGAC               GT[CT]^[GA]AC
HindII        223  GTTGAC               GT[CT]^[GA]AC
KasI           90  GGCGCC               G^GCGCC
KpnI          872  GGTACC               GGTAC^C
MamI         1720  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1993  TCCGGA               T^CCGGA
NaeI          125  GCCGGC               GCC^GGC
NarI           90  GGCGCC               GG^CGCC
NdeI         1203  CATATG               CA^TATG
NgoMIV        125  GCCGGC               G^CCGGC
PaeR7I        383  CTCGAG               C^TCGAG
PmaCI        2241  CACGTG               CAC^GTG
PmlI         2241  CACGTG               CAC^GTG
Psp1406I      548  AACGTT               AA^CGTT
PstI         2274  CTGCAG               CTGCA^G
SfoI           90  GGCGCC               GGC^GCC
SspI          803  AATATT               AAT^ATT
XbaI         2140  TCTAGA               T^CTAGA
XhoI          383  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccII          27  CGCG                 CG^CG
               51  CGCG                
AvaI          383  CTCGAG               C^[CT]CG[GA]G
             1999  CCCGAG              
BalI          763  TGGCCA               TGG^CCA
             2802  TGGCCA              
BamHI        1055  GGATCC               G^GATCC
             2301  GGATCC              
BanII        1585  GAGCTC               G[GA]GC[CT]^C
             1647  GAGCTC              
BbsI         1987  GAAGAC               GAAGAC(2/6)
              388  GTCTTC              
BcnI           76  CCCGG                CC^[GC]GG
               81  CCGGG               
BglI            4  GCCGGAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
               46  GCCGCCGCGGC         
BpuAI        1987  GAAGAC               GAAGAC(2/6)
              388  GTCTTC              
BsoBI         383  CTCGAG               C^[CT]CG[GA]G
             1999  CCCGAG              
BspHI         152  TCATGA               T^CATGA
              641  TCATGA              
BsrFI         125  GCCGGC               [GA]^CCGG[CT]
             2688  ACCGGC              
BstUI          27  CGCG                 CG^CG
               51  CGCG                
Cfr10I        125  GCCGGC               [GA]^CCGG[CT]
             2688  ACCGGC              
DraII         719  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             1601  AGGTCCT             
EcoO109I      719  AGGTCCC              [GA]G^G[ACGT]CC[CT]
             1601  AGGTCCT             
EcoRI        1460  GAATTC               G^AATTC
             2580  GAATTC              
EcoRV         166  GATATC               GAT^ATC
              446  GATATC              
EcoT14I      1627  CCATGG               C^C[AT][AT]GG
             2316  CCATGG              
EcoT22I       421  ATGCAT               ATGCA^T
             2478  ATGCAT              
FauI          105  CCCGC                CCCGC(4/6)
               28  GCGGG               
KspI           26  CCGCGG               CCGC^GG
               50  CCGCGG              
MluNI         763  TGGCCA               TGG^CCA
             2802  TGGCCA              
MscI          763  TGGCCA               TGG^CCA
             2802  TGGCCA              
MvnI           27  CGCG                 CG^CG
               51  CGCG                
NciI           76  CCCGG                CC^[GC]GG
               81  CCGGG               
NcoI         1627  CCATGG               C^CATGG
             2316  CCATGG              
NsiI          421  ATGCAT               ATGCA^T
             2478  ATGCAT              
PflMI        1202  CCATATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1909  CCACTGAATGG         
PpuMI         719  AGGTCCC              [GA]G^G[AT]CC[CT]
             1601  AGGTCCT             
RcaI          152  TCATGA               T^CATGA
              641  TCATGA              
SacI         1585  GAGCTC               GAGCT^C
             1647  GAGCTC              
SacII          26  CCGCGG               CCGC^GG
               50  CCGCGG              
SfcI         1046  CTACAG               C^T[GA][CT]AG
             2274  CTGCAG              
SphI         1127  GCATGC               GCATG^C
             2210  GCATGC              
StyI         1627  CCATGG               C^C[AT][AT]GG
             2316  CCATGG              
Tsp45I        563  GTGAC                ^GT[GC]AC
              961  GTGAC               
Van91I       1202  CCATATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1909  CCACTGAATGG         
XcmI         1220  CCAAAAACTTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2607  CCAGATAAGTAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaII       
BanI        BbvI        BcgI        BfaI        BmyI        
BpmI        BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DsaI        EaeI        EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XhoII       XmnI        
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