Restriction map for pF1KB6016
 (Linear) (Six-base) MAPSORT of: KIBB6016.seq  Check: 2398  from: 1  to: 2823



KIBB6016.orf

 With 182 enzymes: SgfI * 

                        October 2, 2008 10:57  ..

Acc65I G'GTAC_C

Cuts at:      0     872    2823 
   Size:        872    1951
 
AclI AA'CG_TT

Cuts at:      0     549    2823 
   Size:        549    2274
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     848    2054    2411    2542    2823 
   Size:        848    1206     357     131     281

  Fragments arranged by size: 

                1206     848     357     281     131
 
AflII C'TTAA_G

Cuts at:      0    2785    2823 
   Size:       2785      38
 
AflIII A'CryG_T

Cuts at:      0    2242    2823 
   Size:       2242     581
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1796    2823 
   Size:       1796    1027
 
AlwNI CAG_nnn'CTG

Cuts at:      0      40    1702    2312    2823 
   Size:         40    1662     610     511

  Fragments arranged by size: 

                1662     610     511      40
 
ApaLI G'TGCA_C

Cuts at:      0    2004    2823 
   Size:       2004     819
 
ApoI r'AATT_y

Cuts at:      0     685     927    1183    1361    1460    1666    2150    2580 
   Size:        685     242     256     178      99     206     484     430

Cuts at:   2580    2823 
   Size:        243

  Fragments arranged by size: 

                 685     484     430     256     243     242     206     178
                  99
 
AvaI C'yCGr_G

Cuts at:      0     383    1999    2823 
   Size:        383    1616     824

  Fragments arranged by size: 

                1616     824     383
 
BamHI G'GATC_C

Cuts at:      0    1055    2301    2823 
   Size:       1055    1246     522

  Fragments arranged by size: 

                1246    1055     522
 
BanI G'GyrC_C

Cuts at:      0      90     872    2664    2823 
   Size:         90     782    1792     159

  Fragments arranged by size: 

                1792     782     159      90
 
BanII G_rGCy'C

Cuts at:      0    1589    1651    2823 
   Size:       1589      62    1172

  Fragments arranged by size: 

                1589    1172      62
 
BbeI G_GCGC'C

Cuts at:      0      94    2823 
   Size:         94    2729
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     381    1994    2823 
   Size:        381    1613     829

  Fragments arranged by size: 

                1613     829     381
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      44      78    1990    2024    2329    2363    2823 
   Size:         44      34    1912      34     305      34     460

  Fragments arranged by size: 

                1912     460     305      44      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2368    2823 
   Size:       2368     455
 
BclI T'GATC_A

Cuts at:      0     674    2823 
   Size:        674    2149
 
BfrBI ATG'CAT

Cuts at:      0     423    2480    2823 
   Size:        423    2057     343

  Fragments arranged by size: 

                2057     423     343
 
BglI GCCn_nnn'nGGC

Cuts at:      0      10      52    2823 
   Size:         10      42    2771

  Fragments arranged by size: 

                2771      42      10
 
BglII A'GATC_T

Cuts at:      0    1173    2823 
   Size:       1173    1650
 
Bme1580I G_kGCm'C

Cuts at:      0    2008    2669    2823 
   Size:       2008     661     154

  Fragments arranged by size: 

                2008     661     154
 
BmgBI CAC'GTC

Cuts at:      0     141    2823 
   Size:        141    2682
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     472    2281    2823 
   Size:         62     410    1809     542

  Fragments arranged by size: 

                1809     542     410      62
 
Bpu10I CC'TnA_GC

Cuts at:      0    1135    2823 
   Size:       1135    1688
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1665    2424    2535    2823 
   Size:       1665     759     111     288

  Fragments arranged by size: 

                1665     759     288     111
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2679    2823 
   Size:       2679     144
 
BsaAI yAC'GTr

Cuts at:      0    2243    2823 
   Size:       2243     580
 
BsaBI GATnn'nnATC

Cuts at:      0    1724    2823 
   Size:       1724    1099
 
BsaHI Gr'CG_yC

Cuts at:      0      91    2823 
   Size:         91    2732
 
BsaWI w'CCGG_w

Cuts at:      0    1993    2823 
   Size:       1993     830
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     497     527     977    1007    2823 
   Size:        497      30     450      30    1816

  Fragments arranged by size: 

                1816     497     450      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1441    2823 
   Size:       1441    1382
 
BseYI C'CCAG_C

Cuts at:      0    2305    2823 
   Size:       2305     518
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275    1093    1994    2823 
   Size:        275     818     901     829

  Fragments arranged by size: 

                 901     829     818     275
 
BsiEI CG_ry'CG

Cuts at:      0      81    2823 
   Size:         81    2742
 
BsiHKAI G_wGCw'C

Cuts at:      0    1070    1589    1651    2008    2188    2823 
   Size:       1070     519      62     357     180     635

  Fragments arranged by size: 

                1070     635     519     357     180      62
 
BsiWI C'GTAC_G

Cuts at:      0     134    2823 
   Size:        134    2689
 
Bsp1286I G_dGCh'C

Cuts at:      0    1070    1589    1651    2008    2188    2669    2823 
   Size:       1070     519      62     357     180     481     154

  Fragments arranged by size: 

                1070     519     481     357     180     154      62
 
BspEI T'CCGG_A

Cuts at:      0    1993    2823 
   Size:       1993     830
 
BspHI T'CATG_A

Cuts at:      0     152     641    2823 
   Size:        152     489    2182

  Fragments arranged by size: 

                2182     489     152
 
BsrBI CCG'CTC

Cuts at:      0      10    2823 
   Size:         10    2813
 
BsrFI r'CCGG_y

Cuts at:      0     125    2688    2823 
   Size:        125    2563     135

  Fragments arranged by size: 

                2563     135     125
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2273    2823 
   Size:       2273     550
 
BstYI r'GATC_y

Cuts at:      0    1055    1173    2301    2394    2457    2780    2823 
   Size:       1055     118    1128      93      63     323      43

  Fragments arranged by size: 

                1128    1055     323     118      93      63      43
 
Bsu36I CC'TnA_GG

Cuts at:      0    2453    2823 
   Size:       2453     370
 
BtgI C'CryG_G

Cuts at:      0      26      50     186    1627    2316    2823 
   Size:         26      24     136    1441     689     507

  Fragments arranged by size: 

                1441     689     507     136      26      24
 
BtsI GCAGTG_nn'

Cuts at:      0    1300    2006    2283    2823 
   Size:       1300     706     277     540

  Fragments arranged by size: 

                1300     706     540     277
 
DraI TTT'AAA

Cuts at:      0    1316    2823 
   Size:       1316    1507
 
EaeI y'GGCC_r

Cuts at:      0       2      23      78     763    2802    2823 
   Size:          2      21      55     685    2039      21

  Fragments arranged by size: 

                2039     685      55      21      21       2
 
EagI C'GGCC_G

Cuts at:      0      78    2823 
   Size:         78    2745
 
EarI CTCTTCn'nnn_

Cuts at:      0    1628    1814    1856    2030    2823 
   Size:       1628     186      42     174     793

  Fragments arranged by size: 

                1628     793     186     174      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      86    2823 
   Size:         86    2737
 
EcoICRI GAG'CTC

Cuts at:      0    1587    1649    2823 
   Size:       1587      62    1174

  Fragments arranged by size: 

                1587    1174      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     472     848    2054    2281    2411    2542    2823 
   Size:         62     410     376    1206     227     130     131     281

  Fragments arranged by size: 

                1206     410     376     281     227     131     130      62
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1285    2823 
   Size:       1285    1538
 
EcoO109I rG'GnC_Cy

Cuts at:      0     720    1602    2823 
   Size:        720     882    1221

  Fragments arranged by size: 

                1221     882     720
 
EcoRI G'AATT_C

Cuts at:      0    1460    2580    2823 
   Size:       1460    1120     243

  Fragments arranged by size: 

                1460    1120     243
 
EcoRV GAT'ATC

Cuts at:      0     168     448    2823 
   Size:        168     280    2375

  Fragments arranged by size: 

                2375     280     168
 
HaeII r_GCGC'y

Cuts at:      0      94    2823 
   Size:         94    2729
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     455     487     497     529    2366    2379    2398    2411 
   Size:        455      32      10      32    1837      13      19      13

Cuts at:   2411    2823 
   Size:        412

  Fragments arranged by size: 

                1837     455     412      32      32      19      13      13
                  10
 
HincII GTy'rAC

Cuts at:      0     225    2823 
   Size:        225    2598
 
KasI G'GCGC_C

Cuts at:      0      90    2823 
   Size:         90    2733
 
KpnI G_GTAC'C

Cuts at:      0     876    2823 
   Size:        876    1947
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     180    1707    2105    2823 
   Size:        180    1527     398     718

  Fragments arranged by size: 

                1527     718     398     180
 
MscI TGG'CCA

Cuts at:      0     765    2804    2823 
   Size:        765    2039      19

  Fragments arranged by size: 

                2039     765      19
 
MslI CAynn'nnrTG

Cuts at:      0     305     586     626    1126    2823 
   Size:        305     281      40     500    1697

  Fragments arranged by size: 

                1697     500     305     281      40
 
MspA1I CmG'CkG

Cuts at:      0      28      40      52     194     435    2312    2546    2823 
   Size:         28      12      12     142     241    1877     234     277

  Fragments arranged by size: 

                1877     277     241     234     142      28      12      12
            
 
NaeI GCC'GGC

Cuts at:      0     127    2823 
   Size:        127    2696
 
NarI GG'CG_CC

Cuts at:      0      91    2823 
   Size:         91    2732
 
NcoI C'CATG_G

Cuts at:      0    1627    2316    2823 
   Size:       1627     689     507

  Fragments arranged by size: 

                1627     689     507
 
NdeI CA'TA_TG

Cuts at:      0    1204    2823 
   Size:       1204    1619
 
NgoMIV G'CCGG_C

Cuts at:      0     125    2823 
   Size:        125    2698
 
NsiI A_TGCA'T

Cuts at:      0     425    2482    2823 
   Size:        425    2057     341

  Fragments arranged by size: 

                2057     425     341
 
NspI r_CATG'y

Cuts at:      0    1131    1354    2214    2823 
   Size:       1131     223     860     609

  Fragments arranged by size: 

                1131     860     609     223
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1208    1915    2823 
   Size:       1208     707     908

  Fragments arranged by size: 

                1208     908     707
 
PfoI T'CCnGG_A

Cuts at:      0    2297    2416    2823 
   Size:       2297     119     407

  Fragments arranged by size: 

                2297     407     119
 
PmlI CAC'GTG

Cuts at:      0    2243    2823 
   Size:       2243     580
 
PpuMI rG'GwC_Cy

Cuts at:      0     720    1602    2823 
   Size:        720     882    1221

  Fragments arranged by size: 

                1221     882     720
 
PsiI TTA'TAA

Cuts at:      0     415    2823 
   Size:        415    2408
 
PstI C_TGCA'G

Cuts at:      0    2278    2823 
   Size:       2278     545
 
PvuII CAG'CTG

Cuts at:      0      40     194     435    2312    2823 
   Size:         40     154     241    1877     511

  Fragments arranged by size: 

                1877     511     241     154      40
 
SacI G_AGCT'C

Cuts at:      0    1589    1651    2823 
   Size:       1589      62    1172

  Fragments arranged by size: 

                1589    1172      62
 
SacII CC_GC'GG

Cuts at:      0      29      53    2823 
   Size:         29      24    2770

  Fragments arranged by size: 

                2770      29      24
 
SfcI C'TryA_G

Cuts at:      0    1046    2274    2823 
   Size:       1046    1228     549

  Fragments arranged by size: 

                1228    1046     549
 
SfoI GGC'GCC

Cuts at:      0      92    2823 
   Size:         92    2731
 
SmlI C'TyrA_G

Cuts at:      0     383    1644    2403    2550    2785    2823 
   Size:        383    1261     759     147     235      38

  Fragments arranged by size: 

                1261     759     383     235     147      38
 
SphI G_CATG'C

Cuts at:      0    1131    2214    2823 
   Size:       1131    1083     609

  Fragments arranged by size: 

                1131    1083     609
 
SspI AAT'ATT

Cuts at:      0     805    2823 
   Size:        805    2018
 
StyI C'CwwG_G

Cuts at:      0    1627    2316    2823 
   Size:       1627     689     507

  Fragments arranged by size: 

                1627     689     507
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     738    2823 
   Size:        738    2085
 
TatI w'GTAC_w

Cuts at:      0     113    1284    2823 
   Size:        113    1171    1539

  Fragments arranged by size: 

                1539    1171     113
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     977    1009    2823 
   Size:        977      32    1814

  Fragments arranged by size: 

                1814     977      32
 
XbaI T'CTAG_A

Cuts at:      0    2140    2823 
   Size:       2140     683
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1227    2614    2823 
   Size:       1227    1387     209

  Fragments arranged by size: 

                1387    1227     209
 
XhoI C'TCGA_G

Cuts at:      0     383    2823 
   Size:        383    2440
 
XmnI GAAnn'nnTTC

Cuts at:      0     148    1563    2201    2823 
   Size:        148    1415     638     622

  Fragments arranged by size: 

                1415     638     622     148
 

 Enzymes that do cut:

   Acc65I     AclI     AcuI    AflII   AflIII     AlfI    AlwNI    ApaLI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI
    BspHI    BsrBI    BsrFI   BstAPI    BstYI   Bsu36I     BtgI     BtsI
     DraI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NsiI     NspI    PflMI     PfoI     PmlI    PpuMI     PsiI
     PstI    PvuII     SacI    SacII     SfcI     SfoI     SmlI     SphI
     SspI     StyI    TaqII     TatI     TstI     XbaI     XcmI     XhoI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AfeI     AgeI     AhdI     AleI     AloI
     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI     BlpI
     BmrI     BmtI     BplI     BsmI    BsmBI    BspMI    BsrDI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI
    CspCI   DraIII     DrdI     FalI     FseI     FspI    FspAI  HindIII
     HpaI     MfeI     MluI     NheI     NotI     NruI     PacI     PasI
     PciI     PmeI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI
  Tth111I     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com