Restriction map for pF1KB7752
 (Linear) (Six-base) MAPSORT of: KIBB7752.seq  Check: 5550  from: 1  to: 1470



KIBB7752.orf

 With 182 enzymes: SgfI * 

                        October 14, 2008 16:51  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     506     627     772    1276    1470 
   Size:        506     121     145     504     194

  Fragments arranged by size: 

                 506     504     194     145     121
 
AleI CACnn'nnGTG

Cuts at:      0     682     943    1470 
   Size:        682     261     527

  Fragments arranged by size: 

                 682     527     261
 
AlwNI CAG_nnn'CTG

Cuts at:      0     287    1470 
   Size:        287    1183
 
ApoI r'AATT_y

Cuts at:      0     568    1352    1470 
   Size:        568     784     118

  Fragments arranged by size: 

                 784     568     118
 
AvaI C'yCGr_G

Cuts at:      0     987    1364    1407    1470 
   Size:        987     377      43      63

  Fragments arranged by size: 

                 987     377      63      43
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     183     216    1470 
   Size:        183      33    1254

  Fragments arranged by size: 

                1254     183      33
 
BanII G_rGCy'C

Cuts at:      0     479    1306    1315    1414    1470 
   Size:        479     827       9      99      56

  Fragments arranged by size: 

                 827     479      99      56       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     740    1388    1470 
   Size:        740     648      82

  Fragments arranged by size: 

                 740     648      82
 
BfrBI ATG'CAT

Cuts at:      0    1215    1470 
   Size:       1215     255
 
BglI GCCn_nnn'nGGC

Cuts at:      0     517    1166    1470 
   Size:        517     649     304

  Fragments arranged by size: 

                 649     517     304
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     913    1186    1470 
   Size:        913     273     284

  Fragments arranged by size: 

                 913     284     273
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1419    1470 
   Size:       1419      51
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1351    1470 
   Size:       1351     119
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     844    1267    1470 
   Size:        844     423     203

  Fragments arranged by size: 

                 844     423     203
 
BsaHI Gr'CG_yC

Cuts at:      0     890    1181    1470 
   Size:        890     291     289

  Fragments arranged by size: 

                 890     291     289
 
BsaWI w'CCGG_w

Cuts at:      0    1428    1470 
   Size:       1428      42
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      16      46     611     641     760     790    1470 
   Size:         16      30     565      30     119      30     680

  Fragments arranged by size: 

                 680     565     119      30      30      30      16
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     457    1404    1435    1470 
   Size:         58     399     947      31      35

  Fragments arranged by size: 

                 947     399      58      35      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1141    1470 
   Size:       1141     329
 
BsiEI CG_ry'CG

Cuts at:      0    1165    1470 
   Size:       1165     305
 
BsiHKAI G_wGCw'C

Cuts at:      0    1052    1414    1470 
   Size:       1052     362      56

  Fragments arranged by size: 

                1052     362      56
 
BsmI GAATG_Cn'

Cuts at:      0    1217    1470 
   Size:       1217     253
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1330    1470 
   Size:       1330     140
 
Bsp1286I G_dGCh'C

Cuts at:      0     479    1052    1306    1315    1414    1470 
   Size:        479     573     254       9      99      56

  Fragments arranged by size: 

                 573     479     254      99      56       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1043    1224    1470 
   Size:       1043     181     246

  Fragments arranged by size: 

                1043     246     181
 
BsrBI CCG'CTC

Cuts at:      0     429     986    1369    1470 
   Size:        429     557     383     101

  Fragments arranged by size: 

                 557     429     383     101
 
BsrDI GCAATG_nn'

Cuts at:      0     506    1461    1470 
   Size:        506     955       9

  Fragments arranged by size: 

                 955     506       9
 
BsrFI r'CCGG_y

Cuts at:      0    1160    1284    1470 
   Size:       1160     124     186

  Fragments arranged by size: 

                1160     186     124
 
BsrGI T'GTAC_A

Cuts at:      0    1136    1470 
   Size:       1136     334
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     317     963    1456    1470 
   Size:        317     646     493      14

  Fragments arranged by size: 

                 646     493     317      14
 
BstEII G'GTnAC_C

Cuts at:      0      59    1470 
   Size:         59    1411
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     369    1470 
   Size:        369    1101
 
BstYI r'GATC_y

Cuts at:      0     495    1470 
   Size:        495     975
 
BtgI C'CryG_G

Cuts at:      0     350     362    1470 
   Size:        350      12    1108

  Fragments arranged by size: 

                1108     350      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     330     508    1470 
   Size:        330     178     962

  Fragments arranged by size: 

                 962     330     178
 
BtsI GCAGTG_nn'

Cuts at:      0     160     457    1398    1470 
   Size:        160     297     941      72

  Fragments arranged by size: 

                 941     297     160      72
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0       2      37    1470 
   Size:          2      35    1433

  Fragments arranged by size: 

                1433      35       2
 
EaeI y'GGCC_r

Cuts at:      0      90     359     395     588     671    1162    1470 
   Size:         90     269      36     193      83     491     308

  Fragments arranged by size: 

                 491     308     269     193      90      83      36
 
EagI C'GGCC_G

Cuts at:      0    1162    1470 
   Size:       1162     308
 
EarI CTCTTCn'nnn_

Cuts at:      0     482    1012    1303    1470 
   Size:        482     530     291     167

  Fragments arranged by size: 

                 530     482     291     167
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     450     579    1414    1470 
   Size:        450     129     835      56

  Fragments arranged by size: 

                 835     450     129      56
 
EcoICRI GAG'CTC

Cuts at:      0    1412    1470 
   Size:       1412      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     506     627     772    1276    1351    1470 
   Size:        506     121     145     504      75     119

  Fragments arranged by size: 

                 506     504     145     121     119      75
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     751    1470 
   Size:        751     719
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1443    1470 
   Size:       1443      27
 
EcoRI G'AATT_C

Cuts at:      0    1352    1470 
   Size:       1352     118
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     243    1470 
   Size:        243    1227
 
FseI GG_CCGG'CC

Cuts at:      0    1164    1470 
   Size:       1164     306
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48     760     792    1470 
   Size:         16      32     712      32     678

  Fragments arranged by size: 

                 712     678      32      32      16
 
HincII GTy'rAC

Cuts at:      0     977    1059    1470 
   Size:        977      82     411

  Fragments arranged by size: 

                 977     411      82
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     911    1470 
   Size:        911     559
 
MscI TGG'CCA

Cuts at:      0     361     397     590    1470 
   Size:        361      36     193     880

  Fragments arranged by size: 

                 880     361     193      36
 
MslI CAynn'nnrTG

Cuts at:      0     349     361     682     943    1470 
   Size:        349      12     321     261     527

  Fragments arranged by size: 

                 527     349     321     261      12
 
MspA1I CmG'CkG

Cuts at:      0     197     284     324    1150    1470 
   Size:        197      87      40     826     320

  Fragments arranged by size: 

                 826     320     197      87      40
 
NaeI GCC'GGC

Cuts at:      0    1162    1470 
   Size:       1162     308
 
NcoI C'CATG_G

Cuts at:      0     362    1470 
   Size:        362    1108
 
NgoMIV G'CCGG_C

Cuts at:      0    1160    1470 
   Size:       1160     310
 
NsiI A_TGCA'T

Cuts at:      0    1217    1470 
   Size:       1217     253
 
NspI r_CATG'y

Cuts at:      0     117    1219    1470 
   Size:        117    1102     251

  Fragments arranged by size: 

                1102     251     117
 
PasI CC'CwG_GG

Cuts at:      0     540    1470 
   Size:        540     930
 
PfoI T'CCnGG_A

Cuts at:      0    1328    1470 
   Size:       1328     142
 
PpuMI rG'GwC_Cy

Cuts at:      0    1443    1470 
   Size:       1443      27
 
PstI C_TGCA'G

Cuts at:      0     464     959    1470 
   Size:        464     495     511

  Fragments arranged by size: 

                 511     495     464
 
PvuII CAG'CTG

Cuts at:      0     284     324    1470 
   Size:        284      40    1146

  Fragments arranged by size: 

                1146     284      40
 
SacI G_AGCT'C

Cuts at:      0    1414    1470 
   Size:       1414      56
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1303    1470 
   Size:       1303     167
 
SfcI C'TryA_G

Cuts at:      0     460     955    1470 
   Size:        460     495     515

  Fragments arranged by size: 

                 515     495     460
 
SmlI C'TyrA_G

Cuts at:      0     859    1246    1470 
   Size:        859     387     224

  Fragments arranged by size: 

                 859     387     224
 
StyI C'CwwG_G

Cuts at:      0     362    1278    1470 
   Size:        362     916     192

  Fragments arranged by size: 

                 916     362     192
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     612    1470 
   Size:        612     858
 
TatI w'GTAC_w

Cuts at:      0    1136    1470 
   Size:       1136     334
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     611     643    1166    1198    1470 
   Size:        611      32     523      32     272

  Fragments arranged by size: 

                 611     523     272      32      32
 
XbaI T'CTAG_A

Cuts at:      0     375    1470 
   Size:        375    1095
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     681     900    1470 
   Size:        681     219     570

  Fragments arranged by size: 

                 681     570     219
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApoI     AvaI     BaeI    BanII     BbsI
    BfrBI     BglI     BmrI     BplI     BpmI    BpuEI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI    CspCI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI
    Hin4I   HincII     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
   NgoMIV     NsiI     NspI     PasI     PfoI    PpuMI     PstI    PvuII
     SacI     SapI     SfcI     SmlI     StyI    TaqII     TatI     TstI
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BglII     BlpI Bme1580I    BmgBI     BmtI   Bpu10I     BsaI
    BsaAI    BsaBI    BseYI    BsiWI    BspEI    BspHI   BssHII    BssSI
    BstBI  BstZ17I   Bsu36I     ClaI     DraI   DraIII     DrdI    EcoRV
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NarI     NdeI     NheI     NotI     NruI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XhoI     XmaI
     XmnI     ZraI
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