Restriction map for pF1KB0470
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcsI        
AflII       AflIII      AgeI        AhdI        ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AvrII       BbeI        BcgI        BfrI        BglII       
BlnI        BsiWI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstUI       BstZ17I     ClaI        CpoI        
DraI        DrdI        Eam1105I    EcoNI       EcoRI       
EcoRV       EcoT22I     FseI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MfeI        MluI        MunI        MvnI        NarI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        TaqI        Tsp509I     TthHB8I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI         2227  GTAGAC               GT^[AC][GT]AC
AccIII       1796  TCCGGA               T^CCGGA
AfaI         2436  GTAC                 GT^AC
Aor51HI       236  AGCGCT               AGC^GCT
Asp700I      2091  GAATGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         566  TGCGCA               TGC^GCA
BbrPI        1870  CACGTG               CAC^GTG
BciVI        2578  GGATAC               GTATCC(6/5)
BclI         1277  TGATCA               T^GATCA
BsaAI        1870  CACGTG               [CT]AC^GT[GA]
BsaBI        2463  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1796  TCCGGA               [AT]^CCGG[AT]
BseAI        1796  TCCGGA               T^CCGGA
BsiEI        1752  CGGCCG               CG[GA][CT]^CG
BsmBI         478  GAGACG               CGTCTC(1/5)
BsmI          449  GCATTC               GAATGC(1/-1)
BspEI        1796  TCCGGA               T^CCGGA
BsrDI         170  CATTGC               GCAATG(2/0)
BssSI        2078  CTCGTG               CACGAG(-5/-1)
BstEII       2530  GGTCACC              G^GT[ACGT]ACC
BstPI        2530  GGTCACC              G^GT[ACGT]ACC
DraIII       2543  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         1752  CGGCCG               C^GGCCG
EclXI        1752  CGGCCG               C^GGCCG
Eco47III      236  AGCGCT               AGC^GCT
Eco52I       1752  CGGCCG               C^GGCCG
EcoO65I      2530  GGTCACC              G^GT[ACGT]ACC
FbaI         1277  TGATCA               T^GATCA
FspI          566  TGCGCA               TGC^GCA
MamI         2463  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1796  TCCGGA               T^CCGGA
MseI         1129  TTAA                 T^TAA
NheI          381  GCTAGC               G^CTAGC
NspI         2807  GCATGC               [GA]CATG^[CT]
PmaCI        1870  CACGTG               CAC^GTG
PmlI         1870  CACGTG               CAC^GTG
RsaI         2436  GTAC                 GT^AC
SphI         2807  GCATGC               GCATG^C
Tru9I        1129  TTAA                 T^TAA
XmnI         2091  GAATGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcyI         2665  GACGCC               G[GA]^CG[CT]C
             2895  GGCGTC              
Alw44I       2239  GTGCAC               G^TGCAC
             2785  GTGCAC              
ApaLI        2239  GTGCAC               G^TGCAC
             2785  GTGCAC              
BamHI        2427  GGATCC               G^GATCC
             2709  GGATCC              
BbsI         1336  GAAGAC               GAAGAC(2/6)
             1572  GTCTTC              
BbvCI         103  CCTCAGC              CCTCAGC(-5/-2)
             2524  GCTGAGG             
BlpI          504  GCTCAGC              GC^T[ACGT]AGC
             1896  GCTCAGC             
Bpu1102I      504  GCTCAGC              GC^T[ACGT]AGC
             1896  GCTCAGC             
BpuAI        1336  GAAGAC               GAAGAC(2/6)
             1572  GTCTTC              
BsaHI        2665  GACGCC               G[GA]^CG[CT]C
             2895  GGCGTC              
BsaI          691  GAGACC               GGTCTC(1/5)
             1645  GAGACC              
BsgI          199  CTGCAC               GTGCAG(16/14)
               86  GTGCAG              
CelII         504  GCTCAGC              GC^T[ACGT]AGC
             1896  GCTCAGC             
Hin1I        2665  GACGCC               G[GA]^CG[CT]C
             2895  GGCGTC              
NaeI         1750  GCCGGC               GCC^GGC
             2183  GCCGGC              
NgoMIV       1750  GCCGGC               G^CCGGC
             2183  GCCGGC              
PvuII        2188  CAGCTG               CAG^CTG
             2941  CAGCTG              
SapI         1840  GAAGAGC              GCTCTTC(1/4)
             2689  GCTCTTC             


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AocI        
ApaI        AspHI       AspI        AvaI        AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PflFI       PflMI       PleI        PpuMI       PstI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StuI        StyI        TfiI        TseI        
Tsp45I      TspRI       Tth111I     Van91I      XcmI        
XhoII       XmaCI       XmaI        
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