Restriction map for
pF1KB7600
(Circular) (Six-base) MAPSORT of: pF1KB7600.seq Check: 7879 from: 1 to: 4415
pF1KB7600 4415 bp
With 182 enzymes: SgfI *
September 25, 2008 15:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 229 725 229
Size: 496 3919
AatII G_ACGT'C
Cuts at: 776 776
Size: 4415
AccI GT'mk_AC
Cuts at: 674 1453 674
Size: 779 3636
Acc65I G'GTAC_C
Cuts at: 1431 1431
Size: 4415
AclI AA'CG_TT
Cuts at: 4255 4255
Size: 4415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 2672 3419 3687 2240
Size: 432 747 268 2968
Fragments arranged by size:
2968 747 432 268
AflIII A'CryG_T
Cuts at: 1767 2871 3848 1767
Size: 1104 977 2334
Fragments arranged by size:
2334 1104 977
AgeI A'CCGG_T
Cuts at: 162 2803 162
Size: 2641 1774
AhdI GACnn_n'nnGTC
Cuts at: 231 777 231
Size: 546 3869
AleI CACnn'nnGTG
Cuts at: 145 1168 145
Size: 1023 3392
AlwNI CAG_nnn'CTG
Cuts at: 130 975 1116 1251 1720 3287 130
Size: 845 141 135 469 1567 1258
Fragments arranged by size:
1567 1258 845 469 141 135
ApaI G_GGCC'C
Cuts at: 504 1042 504
Size: 538 3877
ApaLI G'TGCA_C
Cuts at: 3185 3185
Size: 4415
ApoI r'AATT_y
Cuts at: 1419 3768 4024 1419
Size: 2349 256 1810
Fragments arranged by size:
2349 1810 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4415
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
AvaI C'yCGr_G
Cuts at: 1042 1079 1435 1042
Size: 37 356 4022
Fragments arranged by size:
4022 356 37
BamHI G'GATC_C
Cuts at: 1440 1440
Size: 4415
BanI G'GyrC_C
Cuts at: 1431 1845 2093 2128 1431
Size: 414 248 35 3718
Fragments arranged by size:
3718 414 248 35
BanII G_rGCy'C
Cuts at: 342 368 504 643 829 1042 1429 2459 342
Size: 26 136 139 186 213 387 1030 2298
Fragments arranged by size:
2298 1030 387 213 186 139 136 26
BbeI G_GCGC'C
Cuts at: 1849 2097 1849
Size: 248 4167
BciVI GTATCCnnnnn_n'
Cuts at: 2313 3074 4415 2313
Size: 761 1341 2313
Fragments arranged by size:
2313 1341 761
BclI T'GATC_A
Cuts at: 209 966 1935 209
Size: 757 969 2689
Fragments arranged by size:
2689 969 757
BglI GCCn_nnn'nGGC
Cuts at: 121 739 1303 121
Size: 618 564 3233
Fragments arranged by size:
3233 618 564
BglII A'GATC_T
Cuts at: 1930 3967 1930
Size: 2037 2378
BlpI GC'TnA_GC
Cuts at: 341 1012 1527 341
Size: 671 515 3229
Fragments arranged by size:
3229 671 515
Bme1580I G_kGCm'C
Cuts at: 504 903 1042 2040 2133 3189 504
Size: 399 139 998 93 1056 1730
Fragments arranged by size:
1730 1056 998 399 139 93
BmrI ACTGGGnnnn_n'
Cuts at: 170 1627 1708 2043 4187 170
Size: 1457 81 335 2144 398
Fragments arranged by size:
2144 1457 398 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2782 2782
Size: 4415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 1018 1198 382
Size: 636 180 3599
Fragments arranged by size:
3599 636 180
Bpu10I CC'TnA_GC
Cuts at: 934 934
Size: 4415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1591 2470 2962 3260 1591
Size: 879 492 298 2746
Fragments arranged by size:
2746 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4119 4119
Size: 4415
BsaAI yAC'GTr
Cuts at: 1352 1768 1352
Size: 416 3999
BsaBI GATnn'nnATC
Cuts at: 6 1929 3966 6
Size: 1923 2037 455
Fragments arranged by size:
2037 1923 455
BsaHI Gr'CG_yC
Cuts at: 623 773 1846 2094 2796 4292 623
Size: 150 1073 248 702 1496 746
Fragments arranged by size:
1496 1073 746 702 248 150
BsaWI w'CCGG_w
Cuts at: 162 1604 2125 2803 3077 3224 3635 162
Size: 1442 521 678 274 147 411 942
Fragments arranged by size:
1442 942 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 466 496 466
Size: 30 4385
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 433 712 1414 433
Size: 279 702 3434
Fragments arranged by size:
3434 702 279
BseYI C'CCAG_C
Cuts at: 684 870 1109 1840 2598 3175 3671 684
Size: 186 239 731 758 577 496 1428
Fragments arranged by size:
1428 758 731 577 496 239 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 805 982 1204 601
Size: 204 177 222 3812
Fragments arranged by size:
3812 222 204 177
BsiEI CG_ry'CG
Cuts at: 104 1957 2003 3211 104
Size: 1853 46 1208 1308
Fragments arranged by size:
1853 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 866 1429 2207 2397 3189 866
Size: 563 778 190 792 2092
Fragments arranged by size:
2092 792 778 563 190
BsiWI C'GTAC_G
Cuts at: 816 816
Size: 4415
BsmI GAATG_Cn'
Cuts at: 1148 1262 1148
Size: 114 4301
Bsp1286I G_dGCh'C
Cuts at: 342 368 504 643 829 866 903 1042 1429
Size: 26 136 139 186 37 37 139 387
Cuts at: 1429 2040 2133 2207 2397 2459 3189 342
Size: 611 93 74 190 62 730 1568
Fragments arranged by size:
1568 730 611 387 190 186 139 139
136 93 74 62 37 37 26
BspEI T'CCGG_A
Cuts at: 3635 3635
Size: 4415
BspHI T'CATG_A
Cuts at: 3591 3591
Size: 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 229 725 1059 1465 1679 1981 2362 229
Size: 496 334 406 214 302 381 2282
Fragments arranged by size:
2282 496 406 381 334 302 214
BsrBI CCG'CTC
Cuts at: 50 587 2707 2761 4245 4410 50
Size: 537 2120 54 1484 165 55
Fragments arranged by size:
2120 1484 537 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1103 2327 1103
Size: 1224 3191
BsrFI r'CCGG_y
Cuts at: 162 616 2413 2594 2803 162
Size: 454 1797 181 209 1774
Fragments arranged by size:
1797 1774 454 209 181
BssSI C'ACGA_G
Cuts at: 1357 2686 3044 1357
Size: 1329 358 2728
Fragments arranged by size:
2728 1329 358
BstAPI GCAn_nnn'nTGC
Cuts at: 244 1792 244
Size: 1548 2867
BstBI TT'CG_AA
Cuts at: 2776 2776
Size: 4415
BstXI CCAn_nnnn'nTGG
Cuts at: 183 759 183
Size: 576 3839
BstYI r'GATC_y
Cuts at: 1 1440 1907 1930 2265 2511 3523 3609 3621
Size: 1439 467 23 335 246 1012 86 12
Cuts at: 3621 3967 1
Size: 346 449
Fragments arranged by size:
1439 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 446 570 446
Size: 124 4291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 732 2365 2546 2709 732
Size: 1633 181 163 2438
Fragments arranged by size:
2438 1633 181 163
BtsI GCAGTG_nn'
Cuts at: 1658 1658
Size: 4415
DraI TTT'AAA
Cuts at: 1414 1414
Size: 4415
DraIII CAC_nnn'GTG
Cuts at: 1320 1320
Size: 4415
DrdI GACnn_nn'nnGTC
Cuts at: 2121 2979 2121
Size: 858 3557
EaeI y'GGCC_r
Cuts at: 194 536 2000 2174 2565 2592 3937 194
Size: 342 1464 174 391 27 1345 672
Fragments arranged by size:
1464 1345 672 391 342 174 27
EagI C'GGCC_G
Cuts at: 2000 2000
Size: 4415
EarI CTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 478 721 2623 2933 3079 4225 478
Size: 243 1902 310 146 1146 668
Fragments arranged by size:
1902 1146 668 310 243 146
EcoICRI GAG'CTC
Cuts at: 1427 1427
Size: 4415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 1018 1198 2240 2672 3419 3687 382
Size: 636 180 1042 432 747 268 1110
Fragments arranged by size:
1110 1042 747 636 432 268 180
EcoO109I rG'GnC_Cy
Cuts at: 311 456 500 930 1038 1039 1155 1209 1248
Size: 145 44 430 108 1 116 54 39
Cuts at: 1248 1305 1363 1554 311
Size: 57 58 191 3172
Fragments arranged by size:
3172 430 191 145 116 108 58 57
54 44 39 1
EcoRI G'AATT_C
Cuts at: 1419 1419
Size: 4415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4415
FspI TGC'GCA
Cuts at: 2196 2196
Size: 4415
HaeII r_GCGC'y
Cuts at: 524 1087 1298 1849 2097 3119 4098 524
Size: 563 211 551 248 1022 979 841
Fragments arranged by size:
1022 979 841 563 551 248 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 466 498 499 531 763 795 2257
Size: 32 217 32 1 32 232 32 1462
Cuts at: 2257 2287 2289 2319 217
Size: 30 2 30 2313
Fragments arranged by size:
2313 1462 232 217 32 32 32 32
30 30 2 1
HincII GTy'rAC
Cuts at: 675 1454 675
Size: 779 3636
HindIII A'AGCT_T
Cuts at: 877 877
Size: 4415
KasI G'GCGC_C
Cuts at: 1845 2093 1845
Size: 248 4167
KpnI G_GTAC'C
Cuts at: 1435 1435
Size: 4415
MfeI C'AATT_G
Cuts at: 544 544
Size: 4415
MluI A'CGCG_T
Cuts at: 3848 3848
Size: 4415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 395 530 674 815 3086 3270 395
Size: 135 144 141 2271 184 1540
Fragments arranged by size:
2271 1540 184 144 141 135
MscI TGG'CCA
Cuts at: 196 538 2176 196
Size: 342 1638 2435
Fragments arranged by size:
2435 1638 342
MslI CAynn'nnrTG
Cuts at: 145 857 1168 2404 2531 145
Size: 712 311 1236 127 2029
Fragments arranged by size:
2029 1236 712 311 127
MspA1I CmG'CkG
Cuts at: 335 572 795 921 1016 1526 1840 2200 3213
Size: 237 223 126 95 510 314 360 1013
Cuts at: 3213 3458 335
Size: 245 1292
Fragments arranged by size:
1292 1013 510 360 314 245 237 223
126 95
NaeI GCC'GGC
Cuts at: 2596 2596
Size: 4415
NarI GG'CG_CC
Cuts at: 1846 2094 1846
Size: 248 4167
NgoMIV G'CCGG_C
Cuts at: 2594 2594
Size: 4415
NspI r_CATG'y
Cuts at: 204 1468 1968 2875 204
Size: 1264 500 907 1744
Fragments arranged by size:
1744 1264 907 500
PasI CC'CwG_GG
Cuts at: 1137 1137
Size: 4415
PciI A'CATG_T
Cuts at: 2871 2871
Size: 4415
PflMI CCAn_nnn'nTGG
Cuts at: 510 1320 510
Size: 810 3605
PfoI T'CCnGG_A
Cuts at: 1097 1244 1097
Size: 147 4268
PmeI GTTT'AAAC
Cuts at: 1414 1414
Size: 4415
PmlI CAC'GTG
Cuts at: 1352 1352
Size: 4415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4383
PpuMI rG'GwC_Cy
Cuts at: 311 456 1209 1248 1363 311
Size: 145 753 39 115 3363
Fragments arranged by size:
3363 753 145 115 39
PspOMI G'GGCC_C
Cuts at: 500 1038 500
Size: 538 3877
PstI C_TGCA'G
Cuts at: 440 920 1121 1462 2147 440
Size: 480 201 341 685 2708
Fragments arranged by size:
2708 685 480 341 201
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4415
PvuII CAG'CTG
Cuts at: 921 1016 1840 2200 921
Size: 95 824 360 3136
Fragments arranged by size:
3136 824 360 95
RsrII CG'GwC_CG
Cuts at: 2610 2610
Size: 4415
SacI G_AGCT'C
Cuts at: 1429 1429
Size: 4415
SacII CC_GC'GG
Cuts at: 573 573
Size: 4415
SalI G'TCGA_C
Cuts at: 673 1452 673
Size: 779 3636
SanDI GG'GwC_CC
Cuts at: 1363 1363
Size: 4415
SapI GCTCTTCn'nnn_
Cuts at: 2438 2648 2438
Size: 210 4205
SbfI CC_TGCA'GG
Cuts at: 1462 1462
Size: 4415
ScaI AGT'ACT
Cuts at: 3932 3932
Size: 4415
SexAI A'CCwGG_T
Cuts at: 1178 1178
Size: 4415
SfcI C'TryA_G
Cuts at: 33 436 916 1117 1458 2143 3136 3327 33
Size: 403 480 201 341 685 993 191 1121
Fragments arranged by size:
1121 993 685 480 403 341 201 191
SfoI GGC'GCC
Cuts at: 1847 2095 1847
Size: 248 4167
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4415
SmaI CCC'GGG
Cuts at: 1081 1437 1081
Size: 356 4059
SmlI C'TyrA_G
Cuts at: 1570 2485 2977 3239 1570
Size: 915 492 262 2746
Fragments arranged by size:
2746 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4415
SphI G_CATG'C
Cuts at: 204 1468 1968 204
Size: 1264 500 2651
Fragments arranged by size:
2651 1264 500
StyI C'CwwG_G
Cuts at: 254 470 524 1549 1903 254
Size: 216 54 1025 354 2766
Fragments arranged by size:
2766 1025 354 216 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 157 556 1998 2131 2590 2799 157
Size: 399 1442 133 459 209 1773
Fragments arranged by size:
1773 1442 459 399 209 133
TatI w'GTAC_w
Cuts at: 3930 3930
Size: 4415
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3932 3964 3932
Size: 32 4383
Tth111I GACn'n_nGTC
Cuts at: 603 2212 603
Size: 1609 2806
XbaI T'CTAG_A
Cuts at: 1446 1446
Size: 4415
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 225 531 1263 1910 225
Size: 306 732 647 2730
Fragments arranged by size:
2730 732 647 306
XmaI C'CCGG_G
Cuts at: 1079 1435 1079
Size: 356 4059
ZraI GAC'GTC
Cuts at: 774 774
Size: 4415
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AloI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BmgBI BmtI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HpaI NcoI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI