Restriction map for pF1KB8986
 (Linear) (Six-base) MAPSORT of: KIBB8986.seq  Check: 5728  from: 1  to: 1161



KIBB8986.orf

 With 182 enzymes: SgfI * 

                        August 7, 2008 14:13  ..

Acc65I G'GTAC_C

Cuts at:      0     476    1161 
   Size:        476     685
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     714    1161 
   Size:        714     447
 
AflII C'TTAA_G

Cuts at:      0     862    1161 
   Size:        862     299
 
AleI CACnn'nnGTG

Cuts at:      0    1133    1161 
   Size:       1133      28
 
AlwNI CAG_nnn'CTG

Cuts at:      0     747    1161 
   Size:        747     414
 
ApoI r'AATT_y

Cuts at:      0     240     403     681    1161 
   Size:        240     163     278     480

  Fragments arranged by size: 

                 480     278     240     163
 
AvaI C'yCGr_G

Cuts at:      0     634    1161 
   Size:        634     527
 
AvrII C'CTAG_G

Cuts at:      0     546    1161 
   Size:        546     615
 
BanI G'GyrC_C

Cuts at:      0     476    1161 
   Size:        476     685
 
BanII G_rGCy'C

Cuts at:      0      55    1161 
   Size:         55    1106
 
BbvCI CC'TCA_GC

Cuts at:      0     149    1161 
   Size:        149    1012
 
BfrBI ATG'CAT

Cuts at:      0     976    1147    1161 
   Size:        976     171      14

  Fragments arranged by size: 

                 976     171      14
 
BglI GCCn_nnn'nGGC

Cuts at:      0      85    1161 
   Size:         85    1076
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     579     878    1161 
   Size:        579     299     283

  Fragments arranged by size: 

                 579     299     283
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     628     874    1161 
   Size:         34     594     246     287

  Fragments arranged by size: 

                 594     287     246      34
 
Bpu10I CC'TnA_GC

Cuts at:      0     149    1161 
   Size:        149    1012
 
BsaHI Gr'CG_yC

Cuts at:      0     533     551    1161 
   Size:        533      18     610

  Fragments arranged by size: 

                 610     533      18
 
BsaWI w'CCGG_w

Cuts at:      0     515    1161 
   Size:        515     646
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1059    1161 
   Size:       1059     102
 
BseYI C'CCAG_C

Cuts at:      0     982    1161 
   Size:        982     179
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     544    1161 
   Size:        544     617
 
BsiHKAI G_wGCw'C

Cuts at:      0      55     665    1161 
   Size:         55     610     496

  Fragments arranged by size: 

                 610     496      55
 
BsmI GAATG_Cn'

Cuts at:      0     622    1161 
   Size:        622     539
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     850    1161 
   Size:        850     311
 
Bsp1286I G_dGCh'C

Cuts at:      0      55     665    1161 
   Size:         55     610     496

  Fragments arranged by size: 

                 610     496      55
 
BsrDI GCAATG_nn'

Cuts at:      0     675    1147    1161 
   Size:        675     472      14

  Fragments arranged by size: 

                 675     472      14
 
BssSI C'ACGA_G

Cuts at:      0    1131    1161 
   Size:       1131      30
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     231     492     573    1018    1161 
   Size:        231     261      81     445     143

  Fragments arranged by size: 

                 445     261     231     143      81
 
BstYI r'GATC_y

Cuts at:      0     903    1161 
   Size:        903     258
 
BtgI C'CryG_G

Cuts at:      0      95    1161 
   Size:         95    1066
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     175    1161 
   Size:        175     986
 
EaeI y'GGCC_r

Cuts at:      0      77     482     494    1161 
   Size:         77     405      12     667

  Fragments arranged by size: 

                 667     405      77      12
 
EarI CTCTTCn'nnn_

Cuts at:      0      43     826    1161 
   Size:         43     783     335

  Fragments arranged by size: 

                 783     335      43
 
EcoICRI GAG'CTC

Cuts at:      0      53    1161 
   Size:         53    1108
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     628     714     874    1161 
   Size:         34     594      86     160     287

  Fragments arranged by size: 

                 594     287     160      86      34
 
EcoO109I rG'GnC_Cy

Cuts at:      0      86     381     639    1161 
   Size:         86     295     258     522

  Fragments arranged by size: 

                 522     295     258      86
 
HaeII r_GCGC'y

Cuts at:      0    1050    1161 
   Size:       1050     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     888     920    1054    1086    1161 
   Size:        888      32     134      32      75

  Fragments arranged by size: 

                 888     134      75      32      32
 
KpnI G_GTAC'C

Cuts at:      0     480    1161 
   Size:        480     681
 
MscI TGG'CCA

Cuts at:      0      79     484    1161 
   Size:         79     405     677

  Fragments arranged by size: 

                 677     405      79
 
MslI CAynn'nnrTG

Cuts at:      0     571    1016    1100    1133    1161 
   Size:        571     445      84      33      28

  Fragments arranged by size: 

                 571     445      84      33      28
 
NcoI C'CATG_G

Cuts at:      0      95    1161 
   Size:         95    1066
 
NsiI A_TGCA'T

Cuts at:      0     978    1149    1161 
   Size:        978     171      12

  Fragments arranged by size: 

                 978     171      12
 
PfoI T'CCnGG_A

Cuts at:      0     644     851    1161 
   Size:        644     207     310

  Fragments arranged by size: 

                 644     310     207
 
PpuMI rG'GwC_Cy

Cuts at:      0     639    1161 
   Size:        639     522
 
RsrII CG'GwC_CG

Cuts at:      0     518    1161 
   Size:        518     643
 
SacI G_AGCT'C

Cuts at:      0      55    1161 
   Size:         55    1106
 
SapI GCTCTTCn'nnn_

Cuts at:      0      43    1161 
   Size:         43    1118
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      85    1161 
   Size:         85    1076
 
SmlI C'TyrA_G

Cuts at:      0     862    1161 
   Size:        862     299
 
StuI AGG'CCT

Cuts at:      0     154    1161 
   Size:        154    1007
 
StyI C'CwwG_G

Cuts at:      0      95     546     621    1161 
   Size:         95     451      75     540

  Fragments arranged by size: 

                 540     451      95      75
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      56    1161 
   Size:         56    1105
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     231    1135    1161 
   Size:        231     904      26

  Fragments arranged by size: 

                 904     231      26
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII     AleI    AlwNI     ApoI     AvaI    AvrII
     BanI    BanII    BbvCI    BfrBI     BglI     BmrI     BpmI   Bpu10I
    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BsrDI    BssSI    BstXI    BstYI     BtgI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I     KpnI     MscI
     MslI     NcoI     NsiI     PfoI    PpuMI    RsrII     SacI     SapI
     SfiI     SmlI     StuI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     BaeI
    BamHI     BbeI     BbsI     BcgI    BciVI     BclI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaXI    BsiEI    BsiWI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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