Restriction map for pF1KB8604
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BsaAI       BsaBI       BseAI       
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MroI        MseI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tsp509I     XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AocI          987  CCTGAGG              CC^T[ACGT]AGG
ApaI          209  GGGCCC               GGGCC^C
AspI          709  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          662  TGGCCA               TGG^CCA
BanI         1147  GGTGCC               G^G[CT][GA]CC
BlpI          196  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      196  GCTGAGC              GC^T[ACGT]AGC
BsaI         1081  GAGACC               GGTCTC(1/5)
BsgI          409  GTGCAG               GTGCAG(16/14)
BsmBI         499  GAGACG               CGTCTC(1/5)
BsrBI          58  CCGCTC               CCGCTC(-3/-3)
BsrFI        1118  GCCGGC               [GA]^CCGG[CT]
BsrI           78  ACTGG                ACTGG(1/-1)
BstAPI          4  GCATCGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         468  GGATCT               [GA]^GATC[CT]
Bsu36I        987  CCTGAGG              CC^T[ACGT]AGG
CelII         196  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1118  GCCGGC               [GA]^CCGG[CT]
DrdI          445  GACTGTGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        987  CCTGAGG              CC^T[ACGT]AGG
HaeII         763  AGCGCC               [GA]GCGC^[CT]
HgaI          315  GACGC                GACGC(5/10)
HinfI         520  GACTC                G^A[ACGT]TC
MflI          468  GGATCT               [GA]^GATC[CT]
MluNI         662  TGGCCA               TGG^CCA
MscI          662  TGGCCA               TGG^CCA
NaeI         1118  GCCGGC               GCC^GGC
NgoMIV       1118  GCCGGC               G^CCGGC
NspI         1059  GCATGC               [GA]CATG^[CT]
PaeR7I         61  CTCGAG               C^TCGAG
PflFI         709  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          520  GACTC                GAGTC(4/5)
PpuMI          50  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI         315  GACGCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         847  CAGCTG               CAG^CTG
SacI         1036  GAGCTC               GAGCT^C
ScaI          527  AGTACT               AGT^ACT
SmlI           61  CTCGAG               C^T[CT][GA]AG
SphI         1059  GCATGC               GCATG^C
Tth111I       709  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          761  CCAGCGCCAAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI           61  CTCGAG               C^TCGAG
XhoII         468  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AatII         384  GACGTC               GACGT^C
              451  GACGTC              
AcyI          384  GACGTC               G[GA]^CG[CT]C
              451  GACGTC              
AlwI         1026  GATCC                GGATC(4/5)
              468  GGATC               
AlwNI         460  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              799  CAGCTCCTG           
BbsI          312  GAAGAC               GAAGAC(2/6)
              603  GAAGAC              
BglI          539  GCCCCCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              742  GCCATCAAGGC         
BpuAI         312  GAAGAC               GAAGAC(2/6)
              603  GAAGAC              
BsaHI         384  GACGTC               G[GA]^CG[CT]C
              451  GACGTC              
BsaWI         668  ACCGGA               [AT]^CCGG[AT]
             1094  ACCGGA              
BsmAI         499  GAGAC                GTCTC(1/5)
             1081  GAGAC               
BstEII        366  GGTCACC              G^GT[ACGT]ACC
              594  GGTGACC             
BstPI         366  GGTCACC              G^GT[ACGT]ACC
              594  GGTGACC             
BstXI         268  CCAGAAGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1162  CCATGGAAGTGG        
DpnII         469  GATC                 ^GATC
             1026  GATC                
EcoO65I       366  GGTCACC              G^GT[ACGT]ACC
              594  GGTGACC             
Hin1I         384  GACGTC               G[GA]^CG[CT]C
              451  GACGTC              
MboI          469  GATC                 ^GATC
             1026  GATC                
NdeII         469  GATC                 ^GATC
             1026  GATC                
PflMI         459  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1157  CCATCCCATGG         
Sau3AI        469  GATC                 ^GATC
             1026  GATC                
Van91I        459  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1157  CCATCCCATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AspHI       
AvaI        AvaII       BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NlaIII      NlaIV       
PstI        RsaI        Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     
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