Restriction map for pF1KB8604
 (Linear) (Six-base) MAPSORT of: KIBB8604.seq  Check: 4254  from: 1  to: 1239



KIBB8604.orf

 With 182 enzymes: SgfI * 

                        February 20, 2009 16:36  ..

AatII G_ACGT'C

Cuts at:      0     388     455    1239 
   Size:        388      67     784

  Fragments arranged by size: 

                 784     388      67
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     622     871    1021    1239 
   Size:        622     249     150     218

  Fragments arranged by size: 

                 622     249     218     150
 
AleI CACnn'nnGTG

Cuts at:      0     406    1239 
   Size:        406     833
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     424     910    1239 
   Size:        424     486     329

  Fragments arranged by size: 

                 486     424     329
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     415     447     766     798    1239 
   Size:        415      32     319      32     441

  Fragments arranged by size: 

                 441     415     319      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     465     804    1239 
   Size:        465     339     435

  Fragments arranged by size: 

                 465     435     339
 
ApaI G_GGCC'C

Cuts at:      0     213    1239 
   Size:        213    1026
 
AvaI C'yCGr_G

Cuts at:      0      61     218     244    1239 
   Size:         61     157      26     995

  Fragments arranged by size: 

                 995     157      61      26
 
BanI G'GyrC_C

Cuts at:      0    1147    1239 
   Size:       1147      92
 
BanII G_rGCy'C

Cuts at:      0      29     213     245     251     559    1040    1239 
   Size:         29     184      32       6     308     481     199

  Fragments arranged by size: 

                 481     308     199     184      32      29       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     319     610    1239 
   Size:        319     291     629

  Fragments arranged by size: 

                 629     319     291
 
BglI GCCn_nnn'nGGC

Cuts at:      0     545     748    1239 
   Size:        545     203     491

  Fragments arranged by size: 

                 545     491     203
 
BlpI GC'TnA_GC

Cuts at:      0     197    1239 
   Size:        197    1042
 
Bme1580I G_kGCm'C

Cuts at:      0     213    1138    1239 
   Size:        213     925     101

  Fragments arranged by size: 

                 925     213     101
 
BmgBI CAC'GTC

Cuts at:      0     492    1239 
   Size:        492     747
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     412     610     649     744     919    1239 
   Size:        184     228     198      39      95     175     320

  Fragments arranged by size: 

                 320     228     198     184     175      95      39
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1075    1239 
   Size:       1075     164
 
BsaHI Gr'CG_yC

Cuts at:      0     385     452    1239 
   Size:        385      67     787

  Fragments arranged by size: 

                 787     385      67
 
BsaWI w'CCGG_w

Cuts at:      0     668    1094    1239 
   Size:        668     426     145

  Fragments arranged by size: 

                 668     426     145
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     415     445    1239 
   Size:        415      30     794

  Fragments arranged by size: 

                 794     415      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     121     130     133     142     205     430     802     805 
   Size:        121       9       3       9      63     225     372       3

Cuts at:    805    1239 
   Size:        434

  Fragments arranged by size: 

                 434     372     225     121      63       9       9       3
                   3
 
BseYI C'CCAG_C

Cuts at:      0     255     953    1104    1114    1239 
   Size:        255     698     151      10     125

  Fragments arranged by size: 

                 698     255     151     125      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430    1239 
   Size:        430     809
 
BsiHKAI G_wGCw'C

Cuts at:      0     905    1040    1078    1239 
   Size:        905     135      38     161

  Fragments arranged by size: 

                 905     161     135      38
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     493    1239 
   Size:        493     746
 
Bsp1286I G_dGCh'C

Cuts at:      0      29     213     245     251     559     905    1040    1078 
   Size:         29     184      32       6     308     346     135      38

Cuts at:   1078    1138    1239 
   Size:         60     101

  Fragments arranged by size: 

                 346     308     184     135     101      60      38      32
                  29       6
 
BsrBI CCG'CTC

Cuts at:      0      60    1239 
   Size:         60    1179
 
BsrFI r'CCGG_y

Cuts at:      0    1118    1239 
   Size:       1118     121
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      10    1239 
   Size:         10    1229
 
BstEII G'GTnAC_C

Cuts at:      0     366     594    1239 
   Size:        366     228     645

  Fragments arranged by size: 

                 645     366     228
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     275    1169    1239 
   Size:        275     894      70

  Fragments arranged by size: 

                 894     275      70
 
BstYI r'GATC_y

Cuts at:      0     468    1239 
   Size:        468     771
 
Bsu36I CC'TnA_GG

Cuts at:      0     988    1239 
   Size:        988     251
 
BtgI C'CryG_G

Cuts at:      0     236     506    1162    1239 
   Size:        236     270     656      77

  Fragments arranged by size: 

                 656     270     236      77
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1209    1239 
   Size:       1209      30
 
BtsI GCAGTG_nn'

Cuts at:      0      37     512    1239 
   Size:         37     475     727

  Fragments arranged by size: 

                 727     475      37
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     451    1239 
   Size:        451     788
 
EaeI y'GGCC_r

Cuts at:      0     503     662     719    1239 
   Size:        503     159      57     520

  Fragments arranged by size: 

                 520     503     159      57
 
EarI CTCTTCn'nnn_

Cuts at:      0     107     119     128     266     934    1239 
   Size:        107      12       9     138     668     305

  Fragments arranged by size: 

                 668     305     138     107      12       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     202     721    1239 
   Size:        202     519     518

  Fragments arranged by size: 

                 519     518     202
 
EcoICRI GAG'CTC

Cuts at:      0    1038    1239 
   Size:       1038     201
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     412     610     622     649     744     871     919 
   Size:        184     228     198      12      27      95     127      48

Cuts at:    919    1021    1239 
   Size:        102     218

  Fragments arranged by size: 

                 228     218     198     184     127     102      95      48
                  27      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51     209     210     462    1239 
   Size:         51     158       1     252     777

  Fragments arranged by size: 

                 777     252     158      51       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     945    1239 
   Size:        945     294
 
HaeII r_GCGC'y

Cuts at:      0     767    1239 
   Size:        767     472
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1040    1239 
   Size:       1040     199
 
MscI TGG'CCA

Cuts at:      0     664    1239 
   Size:        664     575
 
MslI CAynn'nnrTG

Cuts at:      0     406     490    1167    1239 
   Size:        406      84     677      72

  Fragments arranged by size: 

                 677     406      84      72
 
MspA1I CmG'CkG

Cuts at:      0     162     702     849    1071    1239 
   Size:        162     540     147     222     168

  Fragments arranged by size: 

                 540     222     168     162     147
 
NaeI GCC'GGC

Cuts at:      0    1120    1239 
   Size:       1120     119
 
NcoI C'CATG_G

Cuts at:      0     236     506    1162    1239 
   Size:        236     270     656      77

  Fragments arranged by size: 

                 656     270     236      77
 
NgoMIV G'CCGG_C

Cuts at:      0    1118    1239 
   Size:       1118     121
 
NspI r_CATG'y

Cuts at:      0    1063    1239 
   Size:       1063     176
 
PasI CC'CwG_GG

Cuts at:      0     325    1239 
   Size:        325     914
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     465    1163    1239 
   Size:        465     698      76

  Fragments arranged by size: 

                 698     465      76
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     415     447    1239 
   Size:        415      32     792

  Fragments arranged by size: 

                 792     415      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      51    1239 
   Size:         51    1188
 
PshAI GACnn'nnGTC

Cuts at:      0     319    1239 
   Size:        319     920
 
PspOMI G'GGCC_C

Cuts at:      0     209    1239 
   Size:        209    1030
 
PspXI vC'TCGA_Gb

Cuts at:      0      61    1239 
   Size:         61    1178
 
PstI C_TGCA'G

Cuts at:      0     383     809     889    1239 
   Size:        383     426      80     350

  Fragments arranged by size: 

                 426     383     350      80
 
PvuII CAG'CTG

Cuts at:      0     849    1239 
   Size:        849     390
 
SacI G_AGCT'C

Cuts at:      0    1040    1239 
   Size:       1040     199
 
SanDI GG'GwC_CC

Cuts at:      0      51    1239 
   Size:         51    1188
 
ScaI AGT'ACT

Cuts at:      0     529    1239 
   Size:        529     710
 
SfcI C'TryA_G

Cuts at:      0     379     805     885    1239 
   Size:        379     426      80     354

  Fragments arranged by size: 

                 426     379     354      80
 
SmlI C'TyrA_G

Cuts at:      0      61    1239 
   Size:         61    1178
 
SphI G_CATG'C

Cuts at:      0    1063    1239 
   Size:       1063     176
 
StyI C'CwwG_G

Cuts at:      0     236     506     543    1162    1196    1239 
   Size:        236     270      37     619      34      43

  Fragments arranged by size: 

                 619     270     236      43      37      34
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     430    1239 
   Size:        430     809
 
TatI w'GTAC_w

Cuts at:      0     527     965    1239 
   Size:        527     438     274

  Fragments arranged by size: 

                 527     438     274
 
Tth111I GACn'n_nGTC

Cuts at:      0     712    1239 
   Size:        712     527
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     768    1239 
   Size:        768     471
 
XhoI C'TCGA_G

Cuts at:      0      61    1239 
   Size:         61    1178
 
ZraI GAC'GTC

Cuts at:      0     386     453    1239 
   Size:        386      67     786

  Fragments arranged by size: 

                 786     386      67
 

 Enzymes that do cut:

    AatII     AcuI     AleI     AlfI     AloI    AlwNI     ApaI     AvaI
     BanI    BanII     BbsI     BglI     BlpI Bme1580I    BmgBI     BpmI
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BsrBI    BsrFI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I     FalI    HaeII     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NspI     PasI    PflMI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PstI    PvuII     SacI    SanDI
     ScaI     SfcI     SmlI     SphI     StyI    TaqII     TatI  Tth111I
     XcmI     XhoI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsiEI
    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII
    BssSI    BstBI  BstZ17I     ClaI    CspCI     DraI   DraIII     EagI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PfoI     PmeI
     PmlI     PsiI     PsrI     PvuI    RsrII    SacII     SalI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI     TstI     XbaI     XmaI
     XmnI
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