Restriction map for pF1KB8434
 (Linear) (Six-base) MAPSORT of: KIBB8434.seq  Check: 6646  from: 1  to: 414



KIBB8434.orf

 With 182 enzymes: SgfI * 

                        August 7, 2008 14:13  ..

Acc65I G'GTAC_C

Cuts at:      0     389     414 
   Size:        389      25
 
AgeI A'CCGG_T

Cuts at:      0     246     414 
   Size:        246     168
 
AleI CACnn'nnGTG

Cuts at:      0     286     414 
   Size:        286     128
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     275     414 
   Size:        275     139
 
AlwNI CAG_nnn'CTG

Cuts at:      0      99     229     414 
   Size:         99     130     185

  Fragments arranged by size: 

                 185     130      99
 
AvaI C'yCGr_G

Cuts at:      0     263     414 
   Size:        263     151
 
BamHI G'GATC_C

Cuts at:      0     114     414 
   Size:        114     300
 
BanI G'GyrC_C

Cuts at:      0     389     414 
   Size:        389      25
 
BanII G_rGCy'C

Cuts at:      0     215     414 
   Size:        215     199
 
BglI GCCn_nnn'nGGC

Cuts at:      0     181     414 
   Size:        181     233
 
BmgBI CAC'GTC

Cuts at:      0     321     414 
   Size:        321      93
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      20     414 
   Size:         20     394
 
BsaWI w'CCGG_w

Cuts at:      0     246     414 
   Size:        246     168
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     376     414 
   Size:         22     354      38

  Fragments arranged by size: 

                 354      38      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     414 
   Size:        280     134
 
BsiEI CG_ry'CG

Cuts at:      0     247     414 
   Size:        247     167
 
Bsp1286I G_dGCh'C

Cuts at:      0     215     414 
   Size:        215     199
 
BspHI T'CATG_A

Cuts at:      0     158     414 
   Size:        158     256
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     401     414 
   Size:        401      13
 
BsrBI CCG'CTC

Cuts at:      0     366     414 
   Size:        366      48
 
BsrFI r'CCGG_y

Cuts at:      0     106     246     414 
   Size:        106     140     168

  Fragments arranged by size: 

                 168     140     106
 
BssHII G'CGCG_C

Cuts at:      0     144     414 
   Size:        144     270
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     229     414 
   Size:        229     185
 
BstYI r'GATC_y

Cuts at:      0     114     414 
   Size:        114     300
 
BtgI C'CryG_G

Cuts at:      0      62     201     347     404     414 
   Size:         62     139     146      57      10

  Fragments arranged by size: 

                 146     139      62      57      10
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      56     341     414 
   Size:         56     285      73

  Fragments arranged by size: 

                 285      73      56
 
EaeI y'GGCC_r

Cuts at:      0      53     356     414 
   Size:         53     303      58

  Fragments arranged by size: 

                 303      58      53
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     199     414 
   Size:        199     215
 
FspI TGC'GCA

Cuts at:      0     222     397     414 
   Size:        222     175      17

  Fragments arranged by size: 

                 222     175      17
 
HaeII r_GCGC'y

Cuts at:      0      62     176     414 
   Size:         62     114     238

  Fragments arranged by size: 

                 238     114      62
 
HincII GTy'rAC

Cuts at:      0     129     414 
   Size:        129     285
 
KpnI G_GTAC'C

Cuts at:      0     393     414 
   Size:        393      21
 
MscI TGG'CCA

Cuts at:      0      55     414 
   Size:         55     359
 
MslI CAynn'nnrTG

Cuts at:      0     163     286     414 
   Size:        163     123     128

  Fragments arranged by size: 

                 163     128     123
 
MspA1I CmG'CkG

Cuts at:      0      64     414 
   Size:         64     350
 
NaeI GCC'GGC

Cuts at:      0     108     414 
   Size:        108     306
 
NgoMIV G'CCGG_C

Cuts at:      0     106     414 
   Size:        106     308
 
NspI r_CATG'y

Cuts at:      0      24     414 
   Size:         24     390
 
PfoI T'CCnGG_A

Cuts at:      0      98     275     414 
   Size:         98     177     139

  Fragments arranged by size: 

                 177     139      98
 
SacII CC_GC'GG

Cuts at:      0      65     414 
   Size:         65     349
 
SmaI CCC'GGG

Cuts at:      0     265     414 
   Size:        265     149
 
SpeI A'CTAG_T

Cuts at:      0      75     414 
   Size:         75     339
 
SphI G_CATG'C

Cuts at:      0      24     414 
   Size:         24     390
 
XmaI C'CCGG_G

Cuts at:      0     263     414 
   Size:        263     151
 

 Enzymes that do cut:

   Acc65I     AgeI     AleI     AlfI    AlwNI     AvaI    BamHI     BanI
    BanII     BglI    BmgBI     BmrI    BsaWI    BseRI     BsgI    BsiEI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrFI   BssHII   BstAPI    BstYI
     BtgI    BtgZI     EaeI     EciI     FspI    HaeII   HincII     KpnI
     MscI     MslI   MspA1I     NaeI   NgoMIV     NspI     PfoI    SacII
     SmaI     SpeI     SphI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AhdI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI Bme1580I     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseYI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrGI    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI    FspAI    Hin4I  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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