Restriction map for pF1KB9746
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AlwNI       
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspI        AvaI        AvaII       
AviII       AvrII       BalI        BanI        BanII       
BbeI        BbvCI       BbvI        BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRV       EcoT14I     EcoT22I     FbaI        Fnu4HI      
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
ItaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StyI        SwaI        TseI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          101  GTAGAC               GT^[AC][GT]AC
Alw44I       1156  GTGCAC               G^TGCAC
ApaLI        1156  GTGCAC               G^TGCAC
AspHI        1156  GTGCAC               G[AT]GC[AT]^C
BamHI        1016  GGATCC               G^GATCC
BbrPI         113  CACGTG               CAC^GTG
BcnI          500  CCCGG                CC^[GC]GG
BmyI         1156  GTGCAC               G[AGT]GC[ACT]^C
BpmI         1109  CTGGAG               CTGGAG(16/14)
BseRI         544  GAGGAG               GAGGAG(10/8)
BsiHKAI      1156  GTGCAC               G[AT]GC[AT]^C
BsiYI        1020  CCATACTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         1020  CCATACTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I     1156  GTGCAC               G[AGT]GC[ACT]^C
Bsp1407I     1254  TGTACA               T^GTACA
BsrDI         378  GCAATG               GCAATG(2/0)
BsrGI        1254  TGTACA               T^GTACA
BstAPI        878  GCAAGGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        874  GGATG                GGATG(2/0)
BstNI         579  CCTGG                CC^[AT]GG
BstYI        1016  GGATCC               [GA]^GATC[CT]
DpnII        1017  GATC                 ^GATC
DraI         1543  TTTAAA               TTT^AAA
EcoRII        579  CCTGG                ^CC[AT]GG
FokI          874  GGATG                GGATG(9/13)
HapII         501  CCGG                 C^CGG
HpaII         501  CCGG                 C^CGG
MaeIII       1382  GTAAC                ^GT[ACGT]AC
MboI         1017  GATC                 ^GATC
MflI         1016  GGATCC               [GA]^GATC[CT]
MspA1I       1516  CAGCGG               C[AC]G^C[GT]G
MspI          501  CCGG                 C^CGG
MvaI          579  CCTGG                CC^[AT]GG
NciI          500  CCCGG                CC^[GC]GG
NdeII        1017  GATC                 ^GATC
NspI          264  GCATGT               [GA]CATG^[CT]
PleI          151  GACTC                GAGTC(4/5)
PmaCI         113  CACGTG               CAC^GTG
PmlI          113  CACGTG               CAC^GTG
Sau3AI       1017  GATC                 ^GATC
SexAI         578  ACCTGGT              A^CC[AT]GGT
SspBI        1254  TGTACA               T^GTACA
TfiI         1268  GAATC                G^A[AT]TC
XhoII        1016  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AhdI          138  GACCCTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1230  GACTTTTAGTC         
AlwI         1017  GATCC                GGATC(4/5)
             1016  GGATC               
AspEI         138  GACCCTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1230  GACTTTTAGTC         
BbsI          929  GAAGAC               GAAGAC(2/6)
             1563  GAAGAC              
BfaI          481  CTAG                 C^TAG
              653  CTAG                
BlpI          320  GCTCAGC              GC^T[ACGT]AGC
              667  GCTAAGC             
Bpu1102I      320  GCTCAGC              GC^T[ACGT]AGC
              667  GCTAAGC             
BpuAI         929  GAAGAC               GAAGAC(2/6)
             1563  GAAGAC              
BsaAI         113  CACGTG               [CT]AC^GT[GA]
             1159  CACGTA              
BsmI          622  GAATGC               GAATGC(1/-1)
              883  GAATGC              
BssKI         500  CCCGG                ^CC[ACGT]GG
              579  CCTGG               
CelII         320  GCTCAGC              GC^T[ACGT]AGC
              667  GCTAAGC             
Eam1105I      138  GACCCTTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1230  GACTTTTAGTC         
HindIII       473  AAGCTT               A^AGCTT
              493  AAGCTT              
HinfI         151  GACTC                G^A[ACGT]TC
             1268  GAATC               
MaeI          481  CTAG                 C^TAG
              653  CTAG                
MaeII         114  ACGT                 A^CGT
             1160  ACGT                
MwoI          495  GCTTACCCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              878  GCAAGGAATGC         
NdeI          449  CATATG               CA^TATG
             1609  CATATG              
NlaIV           3  GGAACC               GG[ACGT]^[ACGT]CC
             1016  GGATCC              
ScaI          511  AGTACT               AGT^ACT
              985  AGTACT              
ScrFI         500  CCCGG                CC^[ACGT]GG
              579  CCTGG               
TaqI          753  TCGA                 T^CGA
             1239  TCGA                
TthHB8I       753  TCGA                 T^CGA
             1239  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
Asp700I     BsrI        DdeI        EcoRI       FauI        
HaeIII      HphI        MboII       MnlI        MseI        
MslI        NlaIII      RsaI        SfaNI       StuI        
Tru9I       Tsp509I     TspRI       XmnI        
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