Restriction map for pF1KB9703
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DpnII       DraI        DraIII      
DrdI        DsaI        Eam1105I    EarI        Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HinfI       HpaI        KpnI        Ksp632I     KspI        
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MroI        MseI        MslI        MunI        
NcoI        NdeI        NdeII       NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        Sau3AI      
SbfI        SexAI       SfaNI       SfiI        SfuI        
SnaBI       SpeI        Sse8387I    SspBI       StuI        
SwaI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       Tth111I     XbaI        XcmI        XhoI        
XhoII       XmnI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AlwNI         484  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       237  AGCGCT               AGC^GCT
BalI          620  TGGCCA               TGG^CCA
BanII         649  GAGCTC               G[GA]GC[CT]^C
BbsI           90  GTCTTC               GAAGAC(2/6)
BbvCI         642  CCTCAGC              CCTCAGC(-5/-2)
BpuAI          90  GTCTTC               GAAGAC(2/6)
BsgI          655  GTGCAG               GTGCAG(16/14)
BsiEI         224  CGGCCG               CG[GA][CT]^CG
BsmBI         307  GAGACG               CGTCTC(1/5)
BspMI         665  ACCTGC               ACCTGC(4/8)
BsrFI         452  GCCGGC               [GA]^CCGG[CT]
BsrI           87  CCAGT                ACTGG(1/-1)
BstAPI        483  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        567  CATCC                GGATG(2/0)
Cfr10I        452  GCCGGC               [GA]^CCGG[CT]
EagI          224  CGGCCG               C^GGCCG
EclXI         224  CGGCCG               C^GGCCG
Eco47III      237  AGCGCT               AGC^GCT
Eco52I        224  CGGCCG               C^GGCCG
EcoNI         594  CCTCTCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       599  CCAAGG               C^C[AT][AT]GG
FokI          567  CATCC                GGATG(9/13)
MaeII         506  ACGT                 A^CGT
MluNI         620  TGGCCA               TGG^CCA
MscI          620  TGGCCA               TGG^CCA
MspA1I        487  CAGCTG               C[AC]G^C[GT]G
NaeI          452  GCCGGC               GCC^GGC
NgoMIV        452  GCCGGC               G^CCGGC
NheI          234  GCTAGC               G^CTAGC
NspI          524  GCATGC               [GA]CATG^[CT]
PflMI         893  CCAAAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         487  CAGCTG               CAG^CTG
SacI          649  GAGCTC               GAGCT^C
SgrAI         451  CGCCGGCG             C[GA]^CCGG[CT]G
SmlI          865  CTCAAG               C^T[CT][GA]AG
SphI          524  GCATGC               GCATG^C
SspI          877  AATATT               AAT^ATT
StyI          599  CCAAGG               C^C[AT][AT]GG
Van91I        893  CCAAAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AspHI          19  GAGCAC               G[AT]GC[AT]^C
              649  GAGCTC              
BglI          379  GCCGGGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              770  GCCATCGGGGC         
BpmI           16  CTGGAG               CTGGAG(16/14)
              106  CTGGAG              
BsiHKAI        19  GAGCAC               G[AT]GC[AT]^C
              649  GAGCTC              
BsmAI         307  GAGAC                GTCTC(1/5)
              396  GTCTC               
BsmFI          98  GGGAC                GGGAC(10/14)
              731  GGGAC               
HphI          268  GGTGA                GGTGA(8/7)
               80  TCACC               
NlaIII        525  CATG                 CATG^
              845  CATG                
PpuMI          98  GGGACCC              [GA]G^G[AT]CC[CT]
              911  AGGACCC             
PstI          208  CTGCAG               CTGCA^G
              481  CTGCAG              
ScaI          176  AGTACT               AGT^ACT
              390  AGTACT              
SfcI          208  CTGCAG               C^T[GA][CT]AG
              481  CTGCAG              
TaqI           62  TCGA                 T^CGA
              548  TCGA                
TthHB8I        62  TCGA                 T^CGA
              548  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaI        AvaII       BanI        BbeI        BbvI        
BcnI        BfaI        BmyI        BsaHI       BsaJI       
BsiYI       BslI        BsoBI       Bsp1286I    BsrBI       
BssKI       BssSI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DraII       EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       ItaI        KasI        MaeI        
MboII       MnlI        MspI        MvaI        MvnI        
MwoI        NarI        NciI        NlaIV       RsaI        
Sau96I      ScrFI       SfoI        SmaI        TseI        
XmaCI       XmaI        
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