Restriction map for pF1KB9703
 (Linear) (Six-base) MAPSORT of: KIBB9703.seq  Check: 8595  from: 1  to: 984



KIBB9703.orf

 With 182 enzymes: SgfI * 

                        August 7, 2008 14:13  ..

AfeI AGC'GCT

Cuts at:      0     239     984 
   Size:        239     745
 
AlwNI CAG_nnn'CTG

Cuts at:      0     489     984 
   Size:        489     495
 
AvaI C'yCGr_G

Cuts at:      0      28     255     400     738     984 
   Size:         28     227     145     338     246

  Fragments arranged by size: 

                 338     246     227     145      28
 
BanI G'GyrC_C

Cuts at:      0     315     449     719     778     984 
   Size:        315     134     270      59     206

  Fragments arranged by size: 

                 315     270     206     134      59
 
BanII G_rGCy'C

Cuts at:      0     653     984 
   Size:        653     331
 
BbeI G_GCGC'C

Cuts at:      0     319     453     723     984 
   Size:        319     134     270     261

  Fragments arranged by size: 

                 319     270     261     134
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      83     984 
   Size:         83     901
 
BbvCI CC'TCA_GC

Cuts at:      0     643     984 
   Size:        643     341
 
BglI GCCn_nnn'nGGC

Cuts at:      0     385     776     984 
   Size:        385     391     208

  Fragments arranged by size: 

                 391     385     208
 
Bme1580I G_kGCm'C

Cuts at:      0     781     984 
   Size:        781     203
 
BmtI G_CTAG'C

Cuts at:      0     238     984 
   Size:        238     746
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     127     984 
   Size:         37      90     857

  Fragments arranged by size: 

                 857      90      37
 
Bpu10I CC'TnA_GC

Cuts at:      0     157     421     643     984 
   Size:        157     264     222     341

  Fragments arranged by size: 

                 341     264     222     157
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     850     984 
   Size:        850     134
 
BsaHI Gr'CG_yC

Cuts at:      0      41     316     450     542     720     984 
   Size:         41     275     134      92     178     264

  Fragments arranged by size: 

                 275     264     178     134      92      41
 
BseYI C'CCAG_C

Cuts at:      0     796     984 
   Size:        796     188
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676     984 
   Size:        676     308
 
BsiEI CG_ry'CG

Cuts at:      0     227     984 
   Size:        227     757
 
BsiHKAI G_wGCw'C

Cuts at:      0      23     653     984 
   Size:         23     630     331

  Fragments arranged by size: 

                 630     331      23
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     301     984 
   Size:        301     683
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     653     781     984 
   Size:         23     630     128     203

  Fragments arranged by size: 

                 630     203     128      23
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     674     984 
   Size:        674     310
 
BsrBI CCG'CTC

Cuts at:      0     425     430     516     984 
   Size:        425       5      86     468

  Fragments arranged by size: 

                 468     425      86       5
 
BsrFI r'CCGG_y

Cuts at:      0     452     984 
   Size:        452     532
 
BssSI C'ACGA_G

Cuts at:      0     172     357     652     984 
   Size:        172     185     295     332

  Fragments arranged by size: 

                 332     295     185     172
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     489     984 
   Size:        489     495
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     131     984 
   Size:        131     853
 
EaeI y'GGCC_r

Cuts at:      0     224     500     620     984 
   Size:        224     276     120     364

  Fragments arranged by size: 

                 364     276     224     120
 
EagI C'GGCC_G

Cuts at:      0     224     984 
   Size:        224     760
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     145     490     984 
   Size:        145     345     494

  Fragments arranged by size: 

                 494     345     145
 
EcoICRI GAG'CTC

Cuts at:      0     651     984 
   Size:        651     333
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     127     984 
   Size:         37      90     857

  Fragments arranged by size: 

                 857      90      37
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     598     984 
   Size:        598     386
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33      99     261     912     984 
   Size:         33      66     162     651      72

  Fragments arranged by size: 

                 651     162      72      66      33
 
HaeII r_GCGC'y

Cuts at:      0     241     319     453     594     723     984 
   Size:        241      78     134     141     129     261

  Fragments arranged by size: 

                 261     241     141     134     129      78
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     824     856     964     984 
   Size:        824      32     108      20

  Fragments arranged by size: 

                 824     108      32      20
 
KasI G'GCGC_C

Cuts at:      0     315     449     719     984 
   Size:        315     134     270     265

  Fragments arranged by size: 

                 315     270     265     134
 
MscI TGG'CCA

Cuts at:      0     622     984 
   Size:        622     362
 
MspA1I CmG'CkG

Cuts at:      0     489     984 
   Size:        489     495
 
NaeI GCC'GGC

Cuts at:      0     454     984 
   Size:        454     530
 
NarI GG'CG_CC

Cuts at:      0     316     450     720     984 
   Size:        316     134     270     264

  Fragments arranged by size: 

                 316     270     264     134
 
NgoMIV G'CCGG_C

Cuts at:      0     452     984 
   Size:        452     532
 
NheI G'CTAG_C

Cuts at:      0     234     984 
   Size:        234     750
 
NspI r_CATG'y

Cuts at:      0     528     984 
   Size:        528     456
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     899     984 
   Size:        899      85
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     136     168     984 
   Size:        136      32     816

  Fragments arranged by size: 

                 816     136      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      99     912     984 
   Size:         99     813      72

  Fragments arranged by size: 

                 813      99      72
 
PstI C_TGCA'G

Cuts at:      0     212     485     984 
   Size:        212     273     499

  Fragments arranged by size: 

                 499     273     212
 
PvuII CAG'CTG

Cuts at:      0     489     984 
   Size:        489     495
 
SacI G_AGCT'C

Cuts at:      0     653     984 
   Size:        653     331
 
SanDI GG'GwC_CC

Cuts at:      0      99     984 
   Size:         99     885
 
ScaI AGT'ACT

Cuts at:      0     178     392     984 
   Size:        178     214     592

  Fragments arranged by size: 

                 592     214     178
 
SfcI C'TryA_G

Cuts at:      0     208     481     984 
   Size:        208     273     503

  Fragments arranged by size: 

                 503     273     208
 
SfoI GGC'GCC

Cuts at:      0     317     451     721     984 
   Size:        317     134     270     263

  Fragments arranged by size: 

                 317     270     263     134
 
SgrAI Cr'CCGG_yG

Cuts at:      0     452     984 
   Size:        452     532
 
SmaI CCC'GGG

Cuts at:      0      30     402     740     984 
   Size:         30     372     338     244

  Fragments arranged by size: 

                 372     338     244      30
 
SmlI C'TyrA_G

Cuts at:      0     865     984 
   Size:        865     119
 
SphI G_CATG'C

Cuts at:      0     528     984 
   Size:        528     456
 
SrfI GCCC'GGGC

Cuts at:      0     740     984 
   Size:        740     244
 
SspI AAT'ATT

Cuts at:      0     879     984 
   Size:        879     105
 
StyI C'CwwG_G

Cuts at:      0     599     984 
   Size:        599     385
 
TatI w'GTAC_w

Cuts at:      0     176     390     984 
   Size:        176     214     594

  Fragments arranged by size: 

                 594     214     176
 
XmaI C'CCGG_G

Cuts at:      0      28     400     738     984 
   Size:         28     372     338     246

  Fragments arranged by size: 

                 372     338     246      28
 

 Enzymes that do cut:

     AfeI    AlwNI     AvaI     BanI    BanII     BbeI     BbsI    BbvCI
     BglI Bme1580I     BmtI     BpmI   Bpu10I    BpuEI    BsaHI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI
    BssSI   BstAPI    BtgZI     EaeI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I     KasI     MscI   MspA1I     NaeI
     NarI   NgoMIV     NheI     NspI    PflMI     PpiI    PpuMI     PstI
    PvuII     SacI    SanDI     ScaI     SfcI     SfoI    SgrAI     SmaI
     SmlI     SphI     SrfI     SspI     StyI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BplI     BsaI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BsiWI     BsmI    BspEI
    BspHI    BsrDI    BsrGI   BssHII    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EarI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MslI
     NcoI     NdeI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII    SacII     SalI     SapI     SbfI    SexAI     SfiI
     SgfI    SnaBI     SpeI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI
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