Restriction map for pF1KA2033
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

Acc65I      AccII       AccIII      AciI        AclI        
AflIII      AgeI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BcnI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoT22I     FauI        FseI        
FspI        HaeII       HgaI        KasI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MroI        MspA1I      MunI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AflII         687  CTTAAG               C^TTAAG
AlwI          421  GGATC                GGATC(4/5)
AvaII          75  GGACC                G^G[AT]CC
BbsI         1300  GAAGAC               GAAGAC(2/6)
BbvI         1580  GCAGC                GCAGC(8/12)
BclI          378  TGATCA               T^GATCA
BfrI          687  CTTAAG               C^TTAAG
BpuAI        1300  GAAGAC               GAAGAC(2/6)
BsaI          132  GGTCTC               GGTCTC(1/5)
BsaWI        1885  ACCGGA               [AT]^CCGG[AT]
BseRI          58  GAGGAG               GAGGAG(10/8)
BsgI         1578  GTGCAG               GTGCAG(16/14)
BsmAI         133  GTCTC                GTCTC(1/5)
BspHI         282  TCATGA               T^CATGA
BspMI        1612  ACCTGC               ACCTGC(4/8)
BsrDI         218  CATTGC               GCAATG(2/0)
CfoI          184  GCGC                 GCG^C
DsaI          573  CCATGG               C^C[GA][CT]GG
EcoRV         142  GATATC               GAT^ATC
FbaI          378  TGATCA               T^GATCA
Fnu4HI       1580  GCAGC                GC^[ACGT]GC
HhaI          184  GCGC                 GCG^C
HinP1I        184  GCGC                 G^CGC
HpaI         1686  GTTAAC               GTT^AAC
HphI          796  GGTGA                GGTGA(8/7)
ItaI         1580  GCAGC                GC^[ACGT]GC
MwoI          444  GCCTGAAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          573  CCATGG               C^CATGG
PleI           90  GACTC                GAGTC(4/5)
RcaI          282  TCATGA               T^CATGA
SexAI         128  ACCTGGT              A^CC[AT]GGT
SmlI          687  CTTAAG               C^T[CT][GA]AG
SphI          772  GCATGC               GCATG^C
TaqI         1035  TCGA                 T^CGA
TseI         1580  GCAGC                G^C[AT]GC
TspRI         792  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I      1035  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AfaI          365  GTAC                 GT^AC
              622  GTAC                
Asp700I       165  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              579  GAATAATTTC          
BalI           38  TGGCCA               TGG^CCA
              321  TGGCCA              
BfaI          392  CTAG                 C^TAG
              769  CTAG                
BsmFI         101  GGGAC                GGGAC(10/14)
              214  GGGAC               
Cac8I         743  GCAAGC               GC[ACGT]^[ACGT]GC
              772  GCATGC              
Cfr13I         75  GGACC                G^G[ACGT]CC
              548  GGCCC               
EaeI           38  TGGCCA               [CT]^GGCC[GA]
              321  TGGCCA              
EcoT14I       515  CCTTGG               C^C[AT][AT]GG
              573  CCATGG              
HapII        1344  CCGG                 C^CGG
             1886  CCGG                
HpaII        1344  CCGG                 C^CGG
             1886  CCGG                
MaeI          392  CTAG                 C^TAG
              769  CTAG                
MluNI          38  TGGCCA               TGG^CCA
              321  TGGCCA              
MscI           38  TGGCCA               TGG^CCA
              321  TGGCCA              
MspI         1344  CCGG                 C^CGG
             1886  CCGG                
NlaIV          75  GGACCC               GG[ACGT]^[ACGT]CC
              125  GGAACC              
RsaI          365  GTAC                 GT^AC
              622  GTAC                
Sau96I         75  GGACC                G^G[ACGT]CC
              548  GGCCC               
StyI          515  CCTTGG               C^C[AT][AT]GG
              573  CCATGG              
TfiI          801  GAATC                G^A[AT]TC
             1809  GAATC               
XmnI          165  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              579  GAATAATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AatII       AccI        AcsI        AcyI        AhdI        
AluI        ApoI        AspEI       BpmI        BsaHI       
BsaJI       BsiYI       BslI        BsrI        BssKI       
BstF5I      BstNI       DdeI        DpnII       Eam1105I    
EarI        EcoRI       EcoRII      FokI        HaeIII      
Hin1I       HincII      HindII      HindIII     HinfI       
Ksp632I     MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MvaI        NdeII       
NlaIII      NspI        Sau3AI      ScrFI       SfaNI       
Tru9I       Tsp45I      Tsp509I     
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