Restriction map for pF1KA2033
 (Linear) (Six-base) MAPSORT of: KIAA2033.seq  Check: 4009  from: 1  to: 2238



KIAA2033.orf

 With 182 enzymes: SgfI * 

                        August 7, 2008 14:13  ..

AatII G_ACGT'C

Cuts at:      0     847    1377    1855    2238 
   Size:        847     530     478     383

  Fragments arranged by size: 

                 847     530     478     383
 
AccI GT'mk_AC

Cuts at:      0     867    1203    1371    1623    1707    1875    2211    2238 
   Size:        867     336     168     252      84     168     336      27

  Fragments arranged by size: 

                 867     336     336     252     168     168      84      27
            
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     819     983    1319    1827    2238 
   Size:        819     164     336     508     411

  Fragments arranged by size: 

                 819     508     411     336     164
 
AflII C'TTAA_G

Cuts at:      0     687    2238 
   Size:        687    1551
 
AhdI GACnn_n'nnGTC

Cuts at:      0     836    1088    1172    1508    1844    2012    2180    2238 
   Size:        836     252      84     336     336     168     168      58

  Fragments arranged by size: 

                 836     336     336     252     168     168      84      58
            
 
AleI CACnn'nnGTG

Cuts at:      0     794    2238 
   Size:        794    1444
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     118     150    2238 
   Size:        118      32    2088

  Fragments arranged by size: 

                2088     118      32
 
ApoI r'AATT_y

Cuts at:      0      46     292     536     591    1269    1289    1457    1793 
   Size:         46     246     244      55     678      20     168     336

Cuts at:   1793    1961    2238 
   Size:        168     277

  Fragments arranged by size: 

                 678     336     277     246     244     168     168      55
                  46      20
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1307    2238 
   Size:       1307     931
 
BclI T'GATC_A

Cuts at:      0     378    2238 
   Size:        378    1860
 
BmgBI CAC'GTC

Cuts at:      0     105    2238 
   Size:        105    2133
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     899    1067    1151    1235    1403    1487    1571 
   Size:        133     766     168      84      84     168      84      84

Cuts at:   1571    1655    1991    2075    2159    2238 
   Size:         84     336      84      84      79

  Fragments arranged by size: 

                 766     336     168     168     133      84      84      84
                  84      84      84      84      79
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     138    2238 
   Size:        138    2100
 
BsaHI Gr'CG_yC

Cuts at:      0     844    1374    1852    2238 
   Size:        844     530     478     386

  Fragments arranged by size: 

                 844     530     478     386
 
BsaWI w'CCGG_w

Cuts at:      0    1885    2238 
   Size:       1885     353
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     118     148     160     190    2238 
   Size:        118      30      12      30    2048

  Fragments arranged by size: 

                2048     118      30      30      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73    2238 
   Size:         73    2165
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1599    2238 
   Size:       1599     639
 
BspHI T'CATG_A

Cuts at:      0     282    2238 
   Size:        282    1956
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1621    2238 
   Size:       1621     617
 
BsrDI GCAATG_nn'

Cuts at:      0     217    2238 
   Size:        217    2021
 
BtgI C'CryG_G

Cuts at:      0     573    2238 
   Size:        573    1665
 
EaeI y'GGCC_r

Cuts at:      0      38     321    2238 
   Size:         38     283    1917

  Fragments arranged by size: 

                1917     283      38
 
EarI CTCTTCn'nnn_

Cuts at:      0     153     464     959    2238 
   Size:        153     311     495    1279

  Fragments arranged by size: 

                1279     495     311     153
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     819     899     983    1067    1151    1235    1319 
   Size:        133     686      80      84      84      84      84      84

Cuts at:   1319    1403    1487    1571    1655    1827    1991    2075    2159 
   Size:         84      84      84      84     172     164      84      84

Cuts at:   2159    2238 
   Size:         79

  Fragments arranged by size: 

                 686     172     164     133      84      84      84      84
                  84      84      84      84      84      84      84      80
                  79
 
EcoRI G'AATT_C

Cuts at:      0      46     591    1457    1961    2238 
   Size:         46     545     866     504     277

  Fragments arranged by size: 

                 866     545     504     277      46
 
EcoRV GAT'ATC

Cuts at:      0     144    2238 
   Size:        144    2094
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     189     433     557    2238 
   Size:        189     244     124    1681

  Fragments arranged by size: 

                1681     244     189     124
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     160     192     822     854    1074    1106    1158    1190 
   Size:        160      32     630      32     220      32      52      32

Cuts at:   1190    1494    1526    1662    1694    1830    1862    1998    2030 
   Size:        304      32     136      32     136      32     136      32

Cuts at:   2030    2166    2198    2238 
   Size:        136      32      40

  Fragments arranged by size: 

                 630     304     220     160     136     136     136     136
                  52      40      32      32      32      32      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     179     356    1688    2238 
   Size:        179     177    1332     550

  Fragments arranged by size: 

                1332     550     179     177
 
HindIII A'AGCT_T

Cuts at:      0     426     914    1250    1418    1559    1754    1922    2238 
   Size:        426     488     336     168     141     195     168     316

  Fragments arranged by size: 

                 488     426     336     316     195     168     168     141
            
 
HpaI GTT'AAC

Cuts at:      0    1688    2238 
   Size:       1688     550
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1492    1576    1828    1996    2238 
   Size:       1492      84     252     168     242

  Fragments arranged by size: 

                1492     252     242     168      84
 
MscI TGG'CCA

Cuts at:      0      40     323    2238 
   Size:         40     283    1915

  Fragments arranged by size: 

                1915     283      40
 
MslI CAynn'nnrTG

Cuts at:      0     242     287     794     978    1482    2238 
   Size:        242      45     507     184     504     756

  Fragments arranged by size: 

                 756     507     504     242     184      45
 
NcoI C'CATG_G

Cuts at:      0     573    2238 
   Size:        573    1665
 
NspI r_CATG'y

Cuts at:      0     776     860    1112    1196    1364    1532    1868    2204 
   Size:        776      84     252      84     168     168     336     336

Cuts at:   2204    2238 
   Size:         34

  Fragments arranged by size: 

                 776     336     336     252     168     168      84      84
                  34
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     118     150    2238 
   Size:        118      32    2088

  Fragments arranged by size: 

                2088     118      32
 
PsiI TTA'TAA

Cuts at:      0    2069    2238 
   Size:       2069     169
 
SexAI A'CCwGG_T

Cuts at:      0     128    2238 
   Size:        128    2110
 
SmlI C'TyrA_G

Cuts at:      0     687    2238 
   Size:        687    1551
 
SphI G_CATG'C

Cuts at:      0     776    2238 
   Size:        776    1462
 
StyI C'CwwG_G

Cuts at:      0     515     573    2238 
   Size:        515      58    1665

  Fragments arranged by size: 

                1665     515      58
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     348     481    2238 
   Size:        348     133    1757

  Fragments arranged by size: 

                1757     348     133
 
TatI w'GTAC_w

Cuts at:      0     364     621    2238 
   Size:        364     257    1617

  Fragments arranged by size: 

                1617     364     257
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     496     528    1084    1116    1168    1200    2238 
   Size:        496      32     556      32      52      32    1038

  Fragments arranged by size: 

                1038     556     496      52      32      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     169     583    2238 
   Size:        169     414    1655

  Fragments arranged by size: 

                1655     414     169
 
ZraI GAC'GTC

Cuts at:      0     845    1375    1853    2238 
   Size:        845     530     478     385

  Fragments arranged by size: 

                 845     530     478     385
 

 Enzymes that do cut:

    AatII     AccI     AcuI    AflII     AhdI     AleI     AloI     ApoI
     BbsI     BclI    BmgBI     BpmI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BspHI    BspMI    BsrDI     BtgI     EaeI     EarI
  Eco57MI    EcoRI    EcoRV     FalI    Hin4I   HincII  HindIII     HpaI
     MmeI     MscI     MslI     NcoI     NspI     PpiI     PsiI    SexAI
     SmlI     SphI     StyI    TaqII     TatI     TstI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AfeI   AflIII     AgeI     AlfI    AlwNI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI EcoO109I     FseI     FspI    FspAI    HaeII     KasI
     KpnI     MfeI     MluI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI
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