Restriction map for pF1KB1290
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BfrI        
BglII       BlnI        BpuAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BspHI       BsrDI       BssSI       Bst1107I    
BstAPI      BstXI       BstZ17I     ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoRI       EcoRV       EcoT22I     
FseI        FspI        HindIII     KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NotI        NruI        NsiI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcyI          690  GACGCC               G[GA]^CG[CT]C
AhdI          532  GACAGGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         199  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         2194  GGGCCC               GGGCC^C
Asp700I      1758  GAAGCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         532  GACAGGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          710  TGGCCA               TGG^CCA
BclI         1863  TGATCA               T^GATCA
BlpI          651  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      651  GCTCAGC              GC^T[ACGT]AGC
BsaAI         182  TACGTG               [CT]AC^GT[GA]
BsaHI         690  GACGCC               G[GA]^CG[CT]C
BsaI         1359  GGTCTC               GGTCTC(1/5)
BsmI         1383  GAATGC               GAATGC(1/-1)
Bsp1407I     1930  TGTACA               T^GTACA
BspLU11I     1974  ACATGT               A^CATGT
BspMI        1248  ACCTGC               ACCTGC(4/8)
BsrFI         194  ACCGGC               [GA]^CCGG[CT]
BsrGI        1930  TGTACA               T^GTACA
BssHII       1686  GCGCGC               G^CGCGC
BstBI         561  TTCGAA               TT^CGAA
BstEII       1980  GGTTACC              G^GT[ACGT]ACC
BstPI        1980  GGTTACC              G^GT[ACGT]ACC
CelII         651  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        194  ACCGGC               [GA]^CCGG[CT]
DraII        2194  GGGCCCC              [GA]G^G[ACGT]CC[CT]
Eam1105I      532  GACAGGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        2199  CCTGCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     2194  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I      1980  GGTTACC              G^GT[ACGT]ACC
FbaI         1863  TGATCA               T^GATCA
HgaI          690  GACGC                GACGC(5/10)
Hin1I         690  GACGCC               G[GA]^CG[CT]C
HincII        676  GTTAAC               GT[CT]^[GA]AC
HindII        676  GTTAAC               GT[CT]^[GA]AC
HpaI          676  GTTAAC               GTT^AAC
MluNI         710  TGGCCA               TGG^CCA
MscI          710  TGGCCA               TGG^CCA
NheI          240  GCTAGC               G^CTAGC
NspI         1974  ACATGT               [GA]CATG^[CT]
NspV          561  TTCGAA               TT^CGAA
PflMI        1258  CCAGAAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfuI          561  TTCGAA               TT^CGAA
SspBI        1930  TGTACA               T^GTACA
Van91I       1258  CCAGAAGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          793  TCTAGA               T^CTAGA
XcmI         1346  CCACCCCAGCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1758  GAAGCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AocI          835  CCTGAGG              CC^T[ACGT]AGG
             2203  CCTCAGG             
BanI          925  GGCACC               G^G[CT][GA]CC
             1414  GGCACC              
BglI           29  GCCACAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1606  GCCGAGCAGGC         
BsmFI        2185  GGGAC                GGGAC(10/14)
             1342  GTCCC               
Bsu36I        835  CCTGAGG              CC^T[ACGT]AGG
             2203  CCTCAGG             
EaeI           27  CGGCCA               [CT]^GGCC[GA]
              710  TGGCCA              
Eco81I        835  CCTGAGG              CC^T[ACGT]AGG
             2203  CCTCAGG             
FauI         1656  GCGGG                CCCGC(4/6)
             1671  GCGGG               
HaeII        1659  GGCGCT               [GA]GCGC^[CT]
             1784  AGCGCC              
HphI         1876  GGTGA                GGTGA(8/7)
             2126  TCACC               
MseI          677  TTAA                 T^TAA
             1400  TTAA                
MslI          765  CATCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1922  CACTTCCATG          
PvuII         728  CAGCTG               CAG^CTG
             1633  CAGCTG              
SacI          635  GAGCTC               GAGCT^C
             1892  GAGCTC              
SfaNI         512  GCATC                GCATC(5/9)
              751  GCATC               
SmaI          209  CCCGGG               CCC^GGG
             1616  CCCGGG              
SmlI          394  CTTGAG               C^T[CT][GA]AG
             1807  CTTGAG              
TfiI          555  GAATC                G^A[AT]TC
             2157  GAATC               
Tru9I         677  TTAA                 T^TAA
             1400  TTAA                
Tsp45I       2002  GTGAC                ^GT[GC]AC
             2125  GTCAC               
XmaCI         209  CCCGGG               C^CCGGG
             1616  CCCGGG              
XmaI          209  CCCGGG               C^CCGGG
             1616  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        ApoI        AspHI       AvaI        
AvaII       BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsoBI       
Bsp1286I    BsrBI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PleI        
PstI        RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfcI        StyI        TaqI        TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
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