Restriction map for
pF1KB6277
(Circular) (Six-base) MAPSORT of: pF1KB6277.seq Check: 2766 from: 1 to: 5456
pF1KB6277 5456 bp
With 182 enzymes: SgfI *
August 6, 2008 11:24 ..
AccI GT'mk_AC
Cuts at: 2494 2494
Size: 5456
Acc65I G'GTAC_C
Cuts at: 2472 2472
Size: 5456
AclI AA'CG_TT
Cuts at: 5296 5296
Size: 5456
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 2139 3281 3713 4460 4728 1159
Size: 980 1142 432 747 268 1887
Fragments arranged by size:
1887 1142 980 747 432 268
AflIII A'CryG_T
Cuts at: 556 2808 3912 4889 556
Size: 2252 1104 977 1123
Fragments arranged by size:
2252 1123 1104 977
AgeI A'CCGG_T
Cuts at: 3844 3844
Size: 5456
AleI CACnn'nnGTG
Cuts at: 2270 2339 2270
Size: 69 5387
AlwNI CAG_nnn'CTG
Cuts at: 501 1279 1332 2077 2761 4328 501
Size: 778 53 745 684 1567 1629
Fragments arranged by size:
1629 1567 778 745 684 53
ApaLI G'TGCA_C
Cuts at: 1733 4226 1733
Size: 2493 2963
ApoI r'AATT_y
Cuts at: 982 1013 1373 2460 4809 5065 982
Size: 31 360 1087 2349 256 1373
Fragments arranged by size:
2349 1373 1087 360 256 31
AseI AT'TA_AT
Cuts at: 20 20
Size: 5456
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5456
AvaI C'yCGr_G
Cuts at: 374 1850 2476 374
Size: 1476 626 3354
Fragments arranged by size:
3354 1476 626
BamHI G'GATC_C
Cuts at: 2481 2481
Size: 5456
BanI G'GyrC_C
Cuts at: 220 1699 2209 2472 2886 3134 3169 220
Size: 1479 510 263 414 248 35 2507
Fragments arranged by size:
2507 1479 510 414 263 248 35
BanII G_rGCy'C
Cuts at: 381 1836 2470 3500 381
Size: 1455 634 1030 2337
Fragments arranged by size:
2337 1455 1030 634
BbeI G_GCGC'C
Cuts at: 2890 3138 2890
Size: 248 5208
BbsI GAAGACnn'nnnn_
Cuts at: 1487 1487
Size: 5456
BbvCI CC'TCA_GC
Cuts at: 2006 2006
Size: 5456
BciVI GTATCCnnnnn_n'
Cuts at: 273 1737 3354 4115 5456 273
Size: 1464 1617 761 1341 273
Fragments arranged by size:
1617 1464 1341 761 273
BclI T'GATC_A
Cuts at: 2976 2976
Size: 5456
BglII A'GATC_T
Cuts at: 2370 2971 5008 2370
Size: 601 2037 2818
Fragments arranged by size:
2818 2037 601
BlpI GC'TnA_GC
Cuts at: 2568 2568
Size: 5456
Bme1580I G_kGCm'C
Cuts at: 1737 2214 3081 3174 4230 1737
Size: 477 867 93 1056 2963
Fragments arranged by size:
2963 1056 867 477 93
BmrI ACTGGGnnnn_n'
Cuts at: 2668 2749 3084 5228 2668
Size: 81 335 2144 2896
Fragments arranged by size:
2896 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3823 3823
Size: 5456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 830 2393 830
Size: 1563 3893
Bpu10I CC'TnA_GC
Cuts at: 1973 2006 1973
Size: 33 5423
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 317 2632 3511 4003 4301 101
Size: 216 2315 879 492 298 1256
Fragments arranged by size:
2315 1256 879 492 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 1312 5160 1312
Size: 3848 1608
BsaAI yAC'GTr
Cuts at: 559 706 2809 559
Size: 147 2103 3206
Fragments arranged by size:
3206 2103 147
BsaBI GATnn'nnATC
Cuts at: 6 2970 5007 6
Size: 2964 2037 455
Fragments arranged by size:
2964 2037 455
BsaHI Gr'CG_yC
Cuts at: 2887 3135 3837 5333 2887
Size: 248 702 1496 3010
Fragments arranged by size:
3010 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1828 2645 3166 3844 4118 4265 4676 1828
Size: 817 521 678 274 147 411 2608
Fragments arranged by size:
2608 817 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 219 249 219
Size: 30 5426
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 671 2012 671
Size: 1341 4115
BseYI C'CCAG_C
Cuts at: 751 1748 2881 3639 4216 4712 751
Size: 997 1133 758 577 496 1495
Fragments arranged by size:
1495 1133 997 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1385 1927 1385
Size: 542 4914
BsiEI CG_ry'CG
Cuts at: 104 2998 3044 4252 104
Size: 2894 46 1208 1308
Fragments arranged by size:
2894 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 381 1509 1737 2470 3248 3438 4230 381
Size: 1128 228 733 778 190 792 1607
Fragments arranged by size:
1607 1128 792 778 733 228 190
BsiWI C'GTAC_G
Cuts at: 1756 1756
Size: 5456
BsmI GAATG_Cn'
Cuts at: 815 1199 1560 815
Size: 384 361 4711
Fragments arranged by size:
4711 384 361
Bsp1286I G_dGCh'C
Cuts at: 381 1509 1737 1836 2214 2470 3081 3174 3248
Size: 1128 228 99 378 256 611 93 74
Cuts at: 3248 3438 3500 4230 381
Size: 190 62 730 1607
Fragments arranged by size:
1607 1128 730 611 378 256 228 190
99 93 74 62
BspEI T'CCGG_A
Cuts at: 4676 4676
Size: 5456
BspHI T'CATG_A
Cuts at: 4632 4632
Size: 5456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2506 2720 3022 3403 2506
Size: 214 302 381 4559
Fragments arranged by size:
4559 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3748 3802 5286 5451 50
Size: 3698 54 1484 165 55
Fragments arranged by size:
3698 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 374 3368 374
Size: 2994 2462
BsrFI r'CCGG_y
Cuts at: 1170 3454 3635 3844 1170
Size: 2284 181 209 2782
Fragments arranged by size:
2782 2284 209 181
BssSI C'ACGA_G
Cuts at: 3727 4085 3727
Size: 358 5098
BstAPI GCAn_nnn'nTGC
Cuts at: 550 1678 2833 550
Size: 1128 1155 3173
Fragments arranged by size:
3173 1155 1128
BstBI TT'CG_AA
Cuts at: 1650 3817 1650
Size: 2167 3289
BstEII G'GTnAC_C
Cuts at: 1356 1356
Size: 5456
BstXI CCAn_nnnn'nTGG
Cuts at: 1432 1616 2174 2338 1432
Size: 184 558 164 4550
Fragments arranged by size:
4550 558 184 164
BstYI r'GATC_y
Cuts at: 1 431 1221 2370 2481 2948 2971 3306 3552
Size: 430 790 1149 111 467 23 335 246
Cuts at: 3552 4564 4650 4662 5008 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1149 1012 790 467 449 430 346 335
246 111 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 263 263
Size: 5456
BtgI C'CryG_G
Cuts at: 107 146 1763 107
Size: 39 1617 3800
Fragments arranged by size:
3800 1617 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3406 3587 3750 3406
Size: 181 163 5112
Fragments arranged by size:
5112 181 163
BtsI GCAGTG_nn'
Cuts at: 929 2699 929
Size: 1770 3686
DraI TTT'AAA
Cuts at: 2455 2455
Size: 5456
DrdI GACnn_nn'nnGTC
Cuts at: 3162 4020 3162
Size: 858 4598
EaeI y'GGCC_r
Cuts at: 143 794 2280 2328 3041 3215 3606 3633 4978
Size: 651 1486 48 713 174 391 27 1345
Cuts at: 4978 143
Size: 621
Fragments arranged by size:
1486 1345 713 651 621 391 174 48
27
EagI C'GGCC_G
Cuts at: 3041 3041
Size: 5456
EarI CTCTTCn'nnn_
Cuts at: 263 1514 2158 3479 3689 263
Size: 1251 644 1321 210 2030
Fragments arranged by size:
2030 1321 1251 644 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3664 3974 4120 5266 3664
Size: 310 146 1146 3854
Fragments arranged by size:
3854 1146 310 146
EcoICRI GAG'CTC
Cuts at: 379 2468 379
Size: 2089 3367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 830 1159 2139 2393 3281 3713 4460 4728 830
Size: 329 980 254 888 432 747 268 1558
Fragments arranged by size:
1558 980 888 747 432 329 268 254
EcoO109I rG'GnC_Cy
Cuts at: 755 1094 1956 2388 2595 755
Size: 339 862 432 207 3616
Fragments arranged by size:
3616 862 432 339 207
EcoRI G'AATT_C
Cuts at: 1373 2460 1373
Size: 1087 4369
EcoRV GAT'ATC
Cuts at: 2233 2233
Size: 5456
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1327 3475 1327
Size: 2148 3308
FspI TGC'GCA
Cuts at: 3237 3237
Size: 5456
HaeII r_GCGC'y
Cuts at: 1755 2890 3138 4160 5139 1755
Size: 1135 248 1022 979 2072
Fragments arranged by size:
2072 1135 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 363 395 516 548 653 685 1290 1322 1638
Size: 32 121 32 105 32 605 32 316
Cuts at: 1638 1670 2133 2165 2175 2207 3298 3328 3330
Size: 32 463 32 10 32 1091 30 2
Cuts at: 3330 3360 363
Size: 30 2459
Fragments arranged by size:
2459 1091 605 463 316 121 105 32
32 32 32 32 32 32 30 30
10 2
HincII GTy'rAC
Cuts at: 1231 2495 1231
Size: 1264 4192
HindIII A'AGCT_T
Cuts at: 653 1073 1199 653
Size: 420 126 4910
Fragments arranged by size:
4910 420 126
KasI G'GCGC_C
Cuts at: 2886 3134 2886
Size: 248 5208
KpnI G_GTAC'C
Cuts at: 2476 2476
Size: 5456
MluI A'CGCG_T
Cuts at: 4889 4889
Size: 5456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 116 1620 4127 4311 116
Size: 1504 2507 184 1261
Fragments arranged by size:
2507 1504 1261 184
MscI TGG'CCA
Cuts at: 145 796 2282 2330 3217 145
Size: 651 1486 48 887 2384
Fragments arranged by size:
2384 1486 887 651 48
MslI CAynn'nnrTG
Cuts at: 145 218 779 2270 2339 3445 3572 145
Size: 73 561 1491 69 1106 127 2029
Fragments arranged by size:
2029 1491 1106 561 127 73 69
MspA1I CmG'CkG
Cuts at: 751 1369 1911 2074 2427 2567 2881 3241 4254
Size: 618 542 163 353 140 314 360 1013
Cuts at: 4254 4499 751
Size: 245 1708
Fragments arranged by size:
1708 1013 618 542 360 353 314 245
163 140
NaeI GCC'GGC
Cuts at: 1172 3637 1172
Size: 2465 2991
NarI GG'CG_CC
Cuts at: 2887 3135 2887
Size: 248 5208
NcoI C'CATG_G
Cuts at: 107 146 1763 107
Size: 39 1617 3800
Fragments arranged by size:
3800 1617 39
NdeI CA'TA_TG
Cuts at: 2067 2067
Size: 5456
NgoMIV G'CCGG_C
Cuts at: 1170 3635 1170
Size: 2465 2991
NspI r_CATG'y
Cuts at: 429 511 2383 2509 3009 3916 429
Size: 82 1872 126 500 907 1969
Fragments arranged by size:
1969 1872 907 500 126 82
PasI CC'CwG_GG
Cuts at: 2214 2214
Size: 5456
PciI A'CATG_T
Cuts at: 3912 3912
Size: 5456
PflMI CCAn_nnn'nTGG
Cuts at: 480 606 2359 480
Size: 126 1753 3577
Fragments arranged by size:
3577 1753 126
PmeI GTTT'AAAC
Cuts at: 2455 2455
Size: 5456
PmlI CAC'GTG
Cuts at: 559 559
Size: 5456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4595 4627 4595
Size: 32 5424
PpuMI rG'GwC_Cy
Cuts at: 755 1094 1956 2388 755
Size: 339 862 432 3823
Fragments arranged by size:
3823 862 432 339
PsiI TTA'TAA
Cuts at: 1403 1403
Size: 5456
PspXI vC'TCGA_Gb
Cuts at: 374 374
Size: 5456
PstI C_TGCA'G
Cuts at: 624 947 1953 1992 2503 3188 624
Size: 323 1006 39 511 685 2892
Fragments arranged by size:
2892 1006 685 511 323 39
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5456
PvuII CAG'CTG
Cuts at: 1369 1911 2074 2427 2881 3241 1369
Size: 542 163 353 454 360 3584
Fragments arranged by size:
3584 542 454 360 353 163
RsrII CG'GwC_CG
Cuts at: 3651 3651
Size: 5456
SacI G_AGCT'C
Cuts at: 381 2470 381
Size: 2089 3367
SalI G'TCGA_C
Cuts at: 2493 2493
Size: 5456
SapI GCTCTTCn'nnn_
Cuts at: 1514 3479 3689 1514
Size: 1965 210 3281
Fragments arranged by size:
3281 1965 210
SbfI CC_TGCA'GG
Cuts at: 2503 2503
Size: 5456
ScaI AGT'ACT
Cuts at: 4973 4973
Size: 5456
SexAI A'CCwGG_T
Cuts at: 599 2447 599
Size: 1848 3608
SfcI C'TryA_G
Cuts at: 33 620 943 1949 1988 2499 3184 4177 4368
Size: 587 323 1006 39 511 685 993 191
Cuts at: 4368 33
Size: 1121
Fragments arranged by size:
1121 1006 993 685 587 511 323 191
39
SfoI GGC'GCC
Cuts at: 2888 3136 2888
Size: 248 5208
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5456
SmaI CCC'GGG
Cuts at: 2478 2478
Size: 5456
SmlI C'TyrA_G
Cuts at: 116 332 374 2611 3526 4018 4280 116
Size: 216 42 2237 915 492 262 1292
Fragments arranged by size:
2237 1292 915 492 262 216 42
SnaBI TAC'GTA
Cuts at: 706 706
Size: 5456
SpeI A'CTAG_T
Cuts at: 67 1713 67
Size: 1646 3810
SphI G_CATG'C
Cuts at: 429 2383 2509 3009 429
Size: 1954 126 500 2876
Fragments arranged by size:
2876 1954 500 126
StyI C'CwwG_G
Cuts at: 107 146 1311 1763 2226 2291 2590 2944 107
Size: 39 1165 452 463 65 299 354 2619
Fragments arranged by size:
2619 1165 463 452 354 299 65 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1420 1619 1756 2314 3039 3172 3631 3840 1420
Size: 199 137 558 725 133 459 209 3036
Fragments arranged by size:
3036 725 558 459 209 199 137 133
TatI w'GTAC_w
Cuts at: 4971 4971
Size: 5456
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 548 580 1594 1626 2040 2072 4973 5005 548
Size: 32 1014 32 414 32 2901 32 999
Fragments arranged by size:
2901 1014 999 414 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3253 3253
Size: 5456
XbaI T'CTAG_A
Cuts at: 2487 2487
Size: 5456
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1613 2951 1613
Size: 1338 4118
XhoI C'TCGA_G
Cuts at: 374 374
Size: 5456
XmaI C'CCGG_G
Cuts at: 2476 2476
Size: 5456
XmnI GAAnn'nnTTC
Cuts at: 213 1377 213
Size: 1164 4292
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PsiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PfoI PshAI PspOMI PsrI SacII SanDI SfiI
SgrAI SrfI SspI StuI SwaI ZraI