Restriction map for pF1KE0197
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# NO CUT ENZYMES (  196 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      Bst1107I    
BstAPI      BstEII      BstF5I      BstPI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       Cfr10I      
ClaI        CpoI        DpnII       DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HaeIII      HgaI        Hin1I       HincII      
HindII      HpaI        HphI        KasI        KpnI        
KspI        MaeI        MaeIII      MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MunI        NaeI        
NarI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tsp45I      TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


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# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII          42  CGCG                 CG^CG
AciI           41  CCGC                 CCGC(-3/-1)
AcsI          143  AAATTC               [GA]^AATT[CT]
AfaI          243  GTAC                 GT^AC
ApoI          143  AAATTC               [GA]^AATT[CT]
AvaII          27  GGTCC                G^G[AT]CC
BbvCI          51  CCTCAGC              CCTCAGC(-5/-2)
BbvI          337  GCTGC                GCAGC(8/12)
BcnI          182  CCCGG                CC^[GC]GG
BmyI          164  GTGCCC               G[AGT]GC[ACT]^C
BpmI          247  CTGGAG               CTGGAG(16/14)
BsaJI           8  CCAAGG               C^C[ACGT][ACGT]GG
BsiYI         341  CCCAGAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          341  CCCAGAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      164  GTGCCC               G[AGT]GC[ACT]^C
BssSI          31  CTCGTG               CACGAG(-5/-1)
BstBI         103  TTCGAA               TT^CGAA
BstNI         246  CCTGG                CC^[AT]GG
BstUI          42  CGCG                 CG^CG
BstXI           8  CCAAGGTCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I         27  GGTCC                G^G[ACGT]CC
DraI          258  TTTAAA               TTT^AAA
EarI          327  GAAGAG               CTCTTC(1/4)
EcoRII        246  CCTGG                ^CC[AT]GG
EcoT14I         8  CCAAGG               C^C[AT][AT]GG
Fnu4HI        337  GCTGC                GC^[ACGT]GC
HapII         183  CCGG                 C^CGG
HindIII       254  AAGCTT               A^AGCTT
HpaII         183  CCGG                 C^CGG
ItaI          337  GCTGC                GC^[ACGT]GC
Ksp632I       327  GAAGAG               CTCTTC(1/4)
MaeII         128  ACGT                 A^CGT
MseI          259  TTAA                 T^TAA
MspI          183  CCGG                 C^CGG
MvaI          246  CCTGG                CC^[AT]GG
MvnI           42  CGCG                 CG^CG
NciI          182  CCCGG                CC^[GC]GG
NspV          103  TTCGAA               TT^CGAA
RsaI          243  GTAC                 GT^AC
Sau96I         27  GGTCC                G^G[ACGT]CC
SfcI           81  CTACAG               C^T[GA][CT]AG
SfuI          103  TTCGAA               TT^CGAA
StyI            8  CCAAGG               C^C[AT][AT]GG
TaqI          104  TCGA                 T^CGA
Tru9I         259  TTAA                 T^TAA
TseI          337  GCTGC                G^C[AT]GC
Tsp509I       144  AATT                 ^AATT
TthHB8I       104  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BssKI         182  CCCGG                ^CC[ACGT]GG
              246  CCTGG               
CfoI           43  GCGC                 GCG^C
              270  GCGC                
DdeI           52  CTCAG                C^T[ACGT]AG
               76  CTGAG               
HhaI           43  GCGC                 GCG^C
              270  GCGC                
HinP1I         43  GCGC                 G^CGC
              270  GCGC                
HinfI          98  GATTC                G^A[ACGT]TC
              234  GATTC               
MnlI           30  CCTC                 CCTC(7/6)
               51  CCTC                
MwoI           36  GCTGACCGCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               80  GCTACAGATGC         
NlaIII         92  CATG                 CATG^
              112  CATG                
ScrFI         182  CCCGG                CC^[ACGT]GG
              246  CCTGG               
SfaNI          86  GATGC                GCATC(5/9)
              135  GCATC               
TfiI           98  GATTC                G^A[AT]TC
              234  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

AluI        MboII       
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