Restriction map for pF1KE0197
 (Linear) (Six-base) MAPSORT of: KIEE0197.seq  Check: 3859  from: 1  to: 357



KIEE0197.orf

 With 182 enzymes: SgfI * 

                        July 15, 2008 09:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     208     357 
   Size:        154      54     149

  Fragments arranged by size: 

                 154     149      54
 
ApoI r'AATT_y

Cuts at:      0     143     357 
   Size:        143     214
 
BbvCI CC'TCA_GC

Cuts at:      0      52     357 
   Size:         52     305
 
Bme1580I G_kGCm'C

Cuts at:      0     168     357 
   Size:        168     189
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     357 
   Size:        268      89
 
Bpu10I CC'TnA_GC

Cuts at:      0      52      76     357 
   Size:         52      24     281

  Fragments arranged by size: 

                 281      52      24
 
Bsp1286I G_dGCh'C

Cuts at:      0     168     357 
   Size:        168     189
 
BssSI C'ACGA_G

Cuts at:      0      31     357 
   Size:         31     326
 
BstBI TT'CG_AA

Cuts at:      0     104     357 
   Size:        104     253
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      15     357 
   Size:         15     342
 
DraI TTT'AAA

Cuts at:      0     260     357 
   Size:        260      97
 
EarI CTCTTCn'nnn_

Cuts at:      0     322     357 
   Size:        322      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     208     268     357 
   Size:        154      54      60      89

  Fragments arranged by size: 

                 154      89      60      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     113     145     357 
   Size:        113      32     212

  Fragments arranged by size: 

                 212     113      32
 
HindIII A'AGCT_T

Cuts at:      0     254     357 
   Size:        254     103
 
SfcI C'TryA_G

Cuts at:      0      81     357 
   Size:         81     276
 
StyI C'CwwG_G

Cuts at:      0       8     357 
   Size:          8     349
 

 Enzymes that do cut:

     AcuI     ApoI    BbvCI Bme1580I     BpmI   Bpu10I Bsp1286I    BssSI
    BstBI    BstXI     DraI     EarI  Eco57MI    Hin4I  HindIII     SfcI
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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