Restriction map for pF1KE0629
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DdeI        
DraI        DraIII      EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        Ksp632I     MaeI        MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MunI        NaeI        NarI        
NcoI        NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PflMI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AflIII        470  ACACGT               A^C[GA][CT]GT
AgeI          523  ACCGGT               A^CCGGT
AspHI         124  GAGCTC               G[AT]GC[AT]^C
BbvI          489  GCAGC                GCAGC(8/12)
BcgI          110  CGAAGGCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI         523  ACCGGT               [AT]^CCGG[AT]
BseRI          79  GAGGAG               GAGGAG(10/8)
BsiHKAI       124  GAGCTC               G[AT]GC[AT]^C
BsmI          228  GCATTC               GAATGC(1/-1)
BsrBI         463  CCGCTC               CCGCTC(-3/-3)
BsrFI         523  ACCGGT               [GA]^CCGG[CT]
BstBI         284  TTCGAA               TT^CGAA
BstNI         588  CCAGG                CC^[AT]GG
CfoI          100  GCGC                 GCG^C
Cfr10I        523  ACCGGT               [GA]^CCGG[CT]
DpnII         375  GATC                 ^GATC
DraII         113  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DrdI           63  GACCGCGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           65  CCGCGG               C^C[GA][CT]GG
EcoO109I      113  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRII        588  CCAGG                ^CC[AT]GG
HaeIII        114  GGCC                 GG^CC
HhaI          100  GCGC                 GCG^C
HinP1I        100  GCGC                 G^CGC
HindIII       343  AAGCTT               A^AGCTT
KspI           65  CCGCGG               CCGC^GG
MaeII         472  ACGT                 A^CGT
MboI          375  GATC                 ^GATC
MvaI          588  CCAGG                CC^[AT]GG
NdeI          327  CATATG               CA^TATG
NdeII         375  GATC                 ^GATC
NspV          284  TTCGAA               TT^CGAA
PinAI         523  ACCGGT               A^CCGGT
PleI          319  GACTC                GAGTC(4/5)
PstI          487  CTGCAG               CTGCA^G
PvuII         266  CAGCTG               CAG^CTG
SacI          124  GAGCTC               GAGCT^C
SacII          65  CCGCGG               CCGC^GG
Sau3AI        375  GATC                 ^GATC
SfaNI         492  GCATC                GCATC(5/9)
SfuI          284  TTCGAA               TT^CGAA
SmlI           16  CTTGAG               C^T[CT][GA]AG
TfiI          287  GAATC                G^A[AT]TC
TseI          489  GCAGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          307  AAATTC               [GA]^AATT[CT]
              583  AAATTC              
AfaI          144  GTAC                 GT^AC
              521  GTAC                
ApoI          307  AAATTC               [GA]^AATT[CT]
              583  AAATTC              
BanII          19  GAGCCC               G[GA]GC[CT]^C
              124  GAGCTC              
BcnI           26  CCGGG                CC^[GC]GG
              428  CCCGG               
BmyI           19  GAGCCC               G[AGT]GC[ACT]^C
              124  GAGCTC              
BsaJI          65  CCGCGG               C^C[ACGT][ACGT]GG
              427  CCCCGG              
BsiYI          90  CCCGCAGGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              427  CCCCGGAACGG         
BslI           90  CCCGCAGGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              427  CCCCGGAACGG         
Bsp1286I       19  GAGCCC               G[AGT]GC[ACT]^C
              124  GAGCTC              
BsrI          146  ACTGG                ACTGG(1/-1)
               54  CCAGT               
BstF5I         86  CATCC                GGATG(2/0)
              139  GGATG               
Cac8I          93  GCAGGC               GC[ACGT]^[ACGT]GC
              224  GCTCGC              
Fnu4HI         97  GCGGC                GC^[ACGT]GC
              489  GCAGC               
FokI           86  CATCC                GGATG(9/13)
              139  GGATG               
HgaI           45  GACGC                GACGC(5/10)
              102  GCGTC               
HinfI         287  GAATC                G^A[ACGT]TC
              319  GACTC               
HphI          444  TCACC                GGTGA(8/7)
              506  TCACC               
ItaI           97  GCGGC                GC^[ACGT]GC
              489  GCAGC               
MboII         574  GAAGA                GAAGA(8/7)
              282  TCTTC               
MspA1I         65  CCGCGG               C[AC]G^C[GT]G
              266  CAGCTG              
NciI           26  CCGGG                CC^[GC]GG
              428  CCCGG               
RsaI          144  GTAC                 GT^AC
              521  GTAC                
SfcI          487  CTGCAG               C^T[GA][CT]AG
              498  CTGTAG              


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BanI        
BsmFI       BssKI       BstUI       Cfr13I      FauI        
HapII       HpaII       MnlI        MseI        MspI        
MvnI        MwoI        NlaIV       Sau96I      ScrFI       
TaqI        Tru9I       Tsp509I     TspRI       TthHB8I     
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