Restriction map for pF1KE1808
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       AccII       AciI        AclI        AcsI        
AflII       AgeI        AhdI        AlwNI       AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BamHI       BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaBI       BsaI        BsgI        
BsiEI       BsiWI       BsmBI       BsmFI       BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstZ17I     
Bsu36I      Cac8I       CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoRI       EcoRV       
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HgaI        HpaI        ItaI        KspI        
MaeI        MamI        MluI        MspA1I      MvnI        
NaeI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PpuMI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SbfI        ScaI        SexAI       SfcI        
SfiI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TseI        Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I        416  GGTACC               G^GTACC
AcyI          929  GGCGCC               G[GA]^CG[CT]C
AflIII        650  ACACGT               A^C[GA][CT]GT
Alw44I        103  GTGCAC               G^TGCAC
AlwI          639  GATCC                GGATC(4/5)
ApaLI         103  GTGCAC               G^TGCAC
Asp700I       864  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       416  GGTACC               G^GTACC
AspHI         103  GTGCAC               G[AT]GC[AT]^C
BalI          297  TGGCCA               TGG^CCA
BanII         470  GGGCTC               G[GA]GC[CT]^C
BbeI          929  GGCGCC               GGCGC^C
BbrPI         651  CACGTG               CAC^GTG
BbsI          592  GTCTTC               GAAGAC(2/6)
BpuAI         592  GTCTTC               GAAGAC(2/6)
BsaAI         651  CACGTG               [CT]AC^GT[GA]
BsaHI         929  GGCGCC               G[GA]^CG[CT]C
BseRI         157  CTCCTC               GAGGAG(10/8)
BsiHKAI       103  GTGCAC               G[AT]GC[AT]^C
BsmI           78  GAATGC               GAATGC(1/-1)
BspMI         743  ACCTGC               ACCTGC(4/8)
BstXI         374  CCATTAGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         638  AGATCC               [GA]^GATC[CT]
Cfr13I        354  GGGCC                G^G[ACGT]CC
DpnII         639  GATC                 ^GATC
DsaI          494  CCATGG               C^C[GA][CT]GG
EcoT14I       494  CCATGG               C^C[AT][AT]GG
Hin1I         929  GGCGCC               G[GA]^CG[CT]C
HincII        765  GTCGAC               GT[CT]^[GA]AC
HindII        765  GTCGAC               GT[CT]^[GA]AC
HindIII       673  AAGCTT               A^AGCTT
KasI          929  GGCGCC               G^GCGCC
KpnI          416  GGTACC               GGTAC^C
MboI          639  GATC                 ^GATC
MflI          638  AGATCC               [GA]^GATC[CT]
MluNI         297  TGGCCA               TGG^CCA
MscI          297  TGGCCA               TGG^CCA
MseI          883  TTAA                 T^TAA
NarI          929  GGCGCC               GG^CGCC
NcoI          494  CCATGG               C^CATGG
NdeII         639  GATC                 ^GATC
PmaCI         651  CACGTG               CAC^GTG
PmlI          651  CACGTG               CAC^GTG
PshAI         966  GACTGTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI          765  GTCGAC               G^TCGAC
Sau3AI        639  GATC                 ^GATC
Sau96I        354  GGGCC                G^G[ACGT]CC
SfoI          929  GGCGCC               GGC^GCC
StyI          494  CCATGG               C^C[AT][AT]GG
Tru9I         883  TTAA                 T^TAA
XcmI          617  CCAGCACCATCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         638  AGATCC               [GA]^GATC[CT]
XmnI          864  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI          202  GTCTAC               GT^[AC][GT]AC
              765  GTCGAC              
AccIII        176  TCCGGA               T^CCGGA
              641  TCCGGA              
BsaWI         176  TCCGGA               [AT]^CCGG[AT]
              641  TCCGGA              
BseAI         176  TCCGGA               T^CCGGA
              641  TCCGGA              
BsmAI         732  GAGAC                GTCTC(1/5)
              964  GAGAC               
BspEI         176  TCCGGA               T^CCGGA
              641  TCCGGA              
BssKI         588  CCTGG                ^CC[ACGT]GG
              899  CCAGG               
BstEII        486  GGTGACC              G^GT[ACGT]ACC
              693  GGTCACC             
BstNI         588  CCTGG                CC^[AT]GG
              899  CCAGG               
BstPI         486  GGTGACC              G^GT[ACGT]ACC
              693  GGTCACC             
CfoI          821  GCGC                 GCG^C
              930  GCGC                
DdeI           69  CTCAG                C^T[ACGT]AG
              580  CTGAG               
EaeI          297  TGGCCA               [CT]^GGCC[GA]
              371  CGGCCA              
EcoO65I       486  GGTGACC              G^GT[ACGT]ACC
              693  GGTCACC             
EcoRII        588  CCTGG                ^CC[AT]GG
              899  CCAGG               
HaeII         820  AGCGCC               [GA]GCGC^[CT]
              929  GGCGCC              
HapII         177  CCGG                 C^CGG
              642  CCGG                
HhaI          821  GCGC                 GCG^C
              930  GCGC                
HinP1I        821  GCGC                 G^CGC
              930  GCGC                
HpaII         177  CCGG                 C^CGG
              642  CCGG                
MaeII          14  ACGT                 A^CGT
              652  ACGT                
MfeI          311  CAATTG               C^AATTG
              942  CAATTG              
MroI          176  TCCGGA               T^CCGGA
              641  TCCGGA              
MslI          375  CATTAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              606  CATGCTGGTG          
MspI          177  CCGG                 C^CGG
              642  CCGG                
MunI          311  CAATTG               C^AATTG
              942  CAATTG              
MvaI          588  CCTGG                CC^[AT]GG
              899  CCAGG               
MwoI          600  GCCCCTCATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              921  GCAACCTCGGC         
SapI          240  GCTCTTC              GCTCTTC(1/4)
              798  GCTCTTC             
ScrFI         588  CCTGG                CC^[ACGT]GG
              899  CCAGG               
TfiI          153  GATTC                G^A[AT]TC
              869  GATTC               
Tsp509I       312  AATT                 ^AATT
              943  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AfaI        AluI        BanI        BmyI        BsaJI       
BsiYI       BslI        Bsp1286I    BsrI        BstF5I      
EarI        FokI        HaeIII      HinfI       HphI        
Ksp632I     MaeIII      MboII       MnlI        NlaIII      
NlaIV       PleI        RsaI        SfaNI       TaqI        
Tsp45I      TspRI       TthHB8I     
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