Restriction map for pF1KE1808
 (Linear) (Six-base) MAPSORT of: KIEE1808.seq  Check: 9526  from: 1  to: 975



KIEE1808.orf

 With 182 enzymes: SgfI * 

                        July 8, 2015 10:32  ..

AccI GT'mk_AC

Cuts at:      0     203     766     975 
   Size:        203     563     209

  Fragments arranged by size: 

                 563     209     203
 
Acc65I G'GTAC_C

Cuts at:      0     416     975 
   Size:        416     559
 
AflIII A'CryG_T

Cuts at:      0     650     975 
   Size:        650     325
 
ApaLI G'TGCA_C

Cuts at:      0     103     975 
   Size:        103     872
 
BanI G'GyrC_C

Cuts at:      0     389     416     929     975 
   Size:        389      27     513      46

  Fragments arranged by size: 

                 513     389      46      27
 
BanII G_rGCy'C

Cuts at:      0     474     975 
   Size:        474     501
 
BbeI G_GCGC'C

Cuts at:      0     933     975 
   Size:        933      42
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     585     975 
   Size:        585     390
 
Bme1580I G_kGCm'C

Cuts at:      0     107     462     975 
   Size:        107     355     513

  Fragments arranged by size: 

                 513     355     107
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     117     124     975 
   Size:        117       7     851

  Fragments arranged by size: 

                 851     117       7
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     552     554     975 
   Size:        552       2     421

  Fragments arranged by size: 

                 552     421       2
 
Bpu10I CC'TnA_GC

Cuts at:      0     580     975 
   Size:        580     395
 
BsaAI yAC'GTr

Cuts at:      0     653     975 
   Size:        653     322
 
BsaHI Gr'CG_yC

Cuts at:      0     930     975 
   Size:        930      45
 
BsaWI w'CCGG_w

Cuts at:      0     176     641     975 
   Size:        176     465     334

  Fragments arranged by size: 

                 465     334     176
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148     975 
   Size:        148     827
 
BsiHKAI G_wGCw'C

Cuts at:      0     107     975 
   Size:        107     868
 
BsmI GAATG_Cn'

Cuts at:      0      84     975 
   Size:         84     891
 
Bsp1286I G_dGCh'C

Cuts at:      0     107     462     474     975 
   Size:        107     355      12     501

  Fragments arranged by size: 

                 501     355     107      12
 
BspEI T'CCGG_A

Cuts at:      0     176     641     975 
   Size:        176     465     334

  Fragments arranged by size: 

                 465     334     176
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     752     975 
   Size:        752     223
 
BstEII G'GTnAC_C

Cuts at:      0     486     693     975 
   Size:        486     207     282

  Fragments arranged by size: 

                 486     282     207
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     381     975 
   Size:        381     594
 
BstYI r'GATC_y

Cuts at:      0     638     975 
   Size:        638     337
 
BtgI C'CryG_G

Cuts at:      0     494     975 
   Size:        494     481
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     412     975 
   Size:        412     563
 
BtsI GCAGTG_nn'

Cuts at:      0     235     975 
   Size:        235     740
 
EaeI y'GGCC_r

Cuts at:      0     297     371     975 
   Size:        297      74     604

  Fragments arranged by size: 

                 604     297      74
 
EarI CTCTTCn'nnn_

Cuts at:      0     247     521     805     859     975 
   Size:        247     274     284      54     116

  Fragments arranged by size: 

                 284     274     247     116      54
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     897     975 
   Size:        897      78
 
HaeII r_GCGC'y

Cuts at:      0     824     933     975 
   Size:        824     109      42

  Fragments arranged by size: 

                 824     109      42
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     174     206     545     577     748     780     975 
   Size:        174      32     339      32     171      32     195

  Fragments arranged by size: 

                 339     195     174     171      32      32      32
 
HincII GTy'rAC

Cuts at:      0     767     975 
   Size:        767     208
 
HindIII A'AGCT_T

Cuts at:      0     673     975 
   Size:        673     302
 
KasI G'GCGC_C

Cuts at:      0     929     975 
   Size:        929      46
 
KpnI G_GTAC'C

Cuts at:      0     420     975 
   Size:        420     555
 
MfeI C'AATT_G

Cuts at:      0     311     942     975 
   Size:        311     631      33

  Fragments arranged by size: 

                 631     311      33
 
MscI TGG'CCA

Cuts at:      0     299     975 
   Size:        299     676
 
MslI CAynn'nnrTG

Cuts at:      0     379     610     975 
   Size:        379     231     365

  Fragments arranged by size: 

                 379     365     231
 
NarI GG'CG_CC

Cuts at:      0     930     975 
   Size:        930      45
 
NcoI C'CATG_G

Cuts at:      0     494     975 
   Size:        494     481
 
PmlI CAC'GTG

Cuts at:      0     653     975 
   Size:        653     322
 
PshAI GACnn'nnGTC

Cuts at:      0     970     975 
   Size:        970       5
 
SalI G'TCGA_C

Cuts at:      0     765     975 
   Size:        765     210
 
SapI GCTCTTCn'nnn_

Cuts at:      0     247     805     975 
   Size:        247     558     170

  Fragments arranged by size: 

                 558     247     170
 
SfoI GGC'GCC

Cuts at:      0     931     975 
   Size:        931      44
 
StyI C'CwwG_G

Cuts at:      0     494     975 
   Size:        494     481
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     226     975 
   Size:        226     749
 
TatI w'GTAC_w

Cuts at:      0     749     975 
   Size:        749     226
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     775     807     975 
   Size:        775      32     168

  Fragments arranged by size: 

                 775     168      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     624     975 
   Size:        624     351
 
XmnI GAAnn'nnTTC

Cuts at:      0     868     975 
   Size:        868     107
 

 Enzymes that do cut:

     AccI   Acc65I   AflIII    ApaLI     BanI    BanII     BbeI     BbsI
 Bme1580I     BmrI     BplI   Bpu10I    BsaAI    BsaHI    BsaWI    BseRI
  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     EaeI     EarI     FalI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MfeI     MscI     MslI     NarI
     NcoI     PmlI    PshAI     SalI     SapI     SfoI     StyI    TaqII
     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BpmI
    BpuEI     BsaI    BsaBI    BsaXI    BseYI     BsgI    BsiEI    BsiWI
    BsmBI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI     HpaI     MluI     MmeI   MspA1I
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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