Restriction map for
pF1KB9626
(Circular) (Six-base) MAPSORT of: pF1KB9626.seq Check: 5693 from: 1 to: 3518
pF1KB9626 3518 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 556 556
Size: 3518
Acc65I G'GTAC_C
Cuts at: 534 534
Size: 3518
AclI AA'CG_TT
Cuts at: 3358 3358
Size: 3518
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1343 1775 2522 2790 1343
Size: 432 747 268 2071
Fragments arranged by size:
2071 747 432 268
AflIII A'CryG_T
Cuts at: 870 1974 2951 870
Size: 1104 977 1437
Fragments arranged by size:
1437 1104 977
AgeI A'CCGG_T
Cuts at: 1906 1906
Size: 3518
AleI CACnn'nnGTG
Cuts at: 196 196
Size: 3518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 433 433
Size: 3518
AlwNI CAG_nnn'CTG
Cuts at: 294 823 2390 294
Size: 529 1567 1422
Fragments arranged by size:
1567 1422 529
ApaLI G'TGCA_C
Cuts at: 2288 2288
Size: 3518
ApoI r'AATT_y
Cuts at: 522 2871 3127 522
Size: 2349 256 913
Fragments arranged by size:
2349 913 256
AscI GG'CGCG_CC
Cuts at: 324 324
Size: 3518
AseI AT'TA_AT
Cuts at: 20 20
Size: 3518
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3518
AvaI C'yCGr_G
Cuts at: 538 538
Size: 3518
BamHI G'GATC_C
Cuts at: 165 543 165
Size: 378 3140
BanI G'GyrC_C
Cuts at: 534 948 1196 1231 534
Size: 414 248 35 2821
Fragments arranged by size:
2821 414 248 35
BanII G_rGCy'C
Cuts at: 407 532 1562 407
Size: 125 1030 2363
Fragments arranged by size:
2363 1030 125
BbeI G_GCGC'C
Cuts at: 952 1200 952
Size: 248 3270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 119 153 119
Size: 34 3484
BciVI GTATCCnnnnn_n'
Cuts at: 1416 2177 3518 1416
Size: 761 1341 1416
Fragments arranged by size:
1416 1341 761
BclI T'GATC_A
Cuts at: 1038 1038
Size: 3518
BglII A'GATC_T
Cuts at: 1033 3070 1033
Size: 2037 1481
BlpI GC'TnA_GC
Cuts at: 295 630 295
Size: 335 3183
Bme1580I G_kGCm'C
Cuts at: 1143 1236 2292 1143
Size: 93 1056 2369
Fragments arranged by size:
2369 1056 93
BmgBI CAC'GTC
Cuts at: 501 510 501
Size: 9 3509
BmrI ACTGGGnnnn_n'
Cuts at: 730 811 1146 3290 730
Size: 81 335 2144 958
Fragments arranged by size:
2144 958 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 289 1885 289
Size: 1596 1922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 241
Size: 3518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1573 2065 2363 694
Size: 879 492 298 1849
Fragments arranged by size:
1849 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3222 3222
Size: 3518
BsaAI yAC'GTr
Cuts at: 871 871
Size: 3518
BsaBI GATnn'nnATC
Cuts at: 6 457 1032 3069 6
Size: 451 575 2037 455
Fragments arranged by size:
2037 575 455 451
BsaHI Gr'CG_yC
Cuts at: 949 1197 1899 3395 949
Size: 248 702 1496 1072
Fragments arranged by size:
1496 1072 702 248
BsaWI w'CCGG_w
Cuts at: 120 168 707 1228 1906 2180 2327 2738 120
Size: 48 539 521 678 274 147 411 900
Fragments arranged by size:
900 678 539 521 411 274 147 48
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 319 247
Size: 72 3446
BseYI C'CCAG_C
Cuts at: 148 943 1701 2278 2774 148
Size: 795 758 577 496 892
Fragments arranged by size:
892 795 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1060 1106 2314 104
Size: 956 46 1208 1308
Fragments arranged by size:
1308 1208 956 46
BsiHKAI G_wGCw'C
Cuts at: 203 407 532 1310 1500 2292 203
Size: 204 125 778 190 792 1429
Fragments arranged by size:
1429 792 778 204 190 125
Bsp1286I G_dGCh'C
Cuts at: 203 407 532 1143 1236 1310 1500 1562 2292
Size: 204 125 611 93 74 190 62 730
Cuts at: 2292 203
Size: 1429
Fragments arranged by size:
1429 730 611 204 190 125 93 74
62
BspEI T'CCGG_A
Cuts at: 120 168 2738 120
Size: 48 2570 900
Fragments arranged by size:
2570 900 48
BspHI T'CATG_A
Cuts at: 2694 2694
Size: 3518
BspMI ACCTGCnnnn'nnnn_
Cuts at: 568 782 1084 1465 568
Size: 214 302 381 2621
Fragments arranged by size:
2621 381 302 214
BsrBI CCG'CTC
Cuts at: 50 344 1810 1864 3348 3513 50
Size: 294 1466 54 1484 165 55
Fragments arranged by size:
1484 1466 294 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1430 1430
Size: 3518
BsrFI r'CCGG_y
Cuts at: 225 320 1516 1697 1906 225
Size: 95 1196 181 209 1837
Fragments arranged by size:
1837 1196 209 181 95
BssHII G'CGCG_C
Cuts at: 324 324
Size: 3518
BssSI C'ACGA_G
Cuts at: 1789 2147 1789
Size: 358 3160
BstAPI GCAn_nnn'nTGC
Cuts at: 895 895
Size: 3518
BstBI TT'CG_AA
Cuts at: 1879 1879
Size: 3518
BstYI r'GATC_y
Cuts at: 1 165 458 543 1010 1033 1368 1614 2626
Size: 164 293 85 467 23 335 246 1012
Cuts at: 2626 2712 2724 3070 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 293 246 164
86 85 23 12
BtgI C'CryG_G
Cuts at: 329 329
Size: 3518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1468 1649 1812 1468
Size: 181 163 3174
Fragments arranged by size:
3174 181 163
BtsI GCAGTG_nn'
Cuts at: 761 761
Size: 3518
DraI TTT'AAA
Cuts at: 517 517
Size: 3518
DrdI GACnn_nn'nnGTC
Cuts at: 1224 2082 1224
Size: 858 2660
EaeI y'GGCC_r
Cuts at: 332 470 1103 1277 1668 1695 3040 332
Size: 138 633 174 391 27 1345 810
Fragments arranged by size:
1345 810 633 391 174 138 27
EagI C'GGCC_G
Cuts at: 1103 1103
Size: 3518
EarI CTCTTCn'nnn_
Cuts at: 1541 1751 1541
Size: 210 3308
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1726 2036 2182 3328 1726
Size: 310 146 1146 1916
Fragments arranged by size:
1916 1146 310 146
EcoICRI GAG'CTC
Cuts at: 405 530 405
Size: 125 3393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1343 1775 2522 2790 241
Size: 1102 432 747 268 969
Fragments arranged by size:
1102 969 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 657 657
Size: 3518
EcoRI G'AATT_C
Cuts at: 522 522
Size: 3518
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1537 1537
Size: 3518
FspI TGC'GCA
Cuts at: 1299 1299
Size: 3518
HaeII r_GCGC'y
Cuts at: 315 952 1200 2222 3201 315
Size: 637 248 1022 979 632
Fragments arranged by size:
1022 979 637 632 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 102 134 157 189 1360 1390 1392 1422 102
Size: 32 23 32 1171 30 2 30 2198
Fragments arranged by size:
2198 1171 32 32 30 30 23 2
HincII GTy'rAC
Cuts at: 557 557
Size: 3518
KasI G'GCGC_C
Cuts at: 948 1196 948
Size: 248 3270
KpnI G_GTAC'C
Cuts at: 538 538
Size: 3518
MluI A'CGCG_T
Cuts at: 2951 2951
Size: 3518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 2189 2373 194
Size: 1995 184 1339
Fragments arranged by size:
1995 1339 184
MscI TGG'CCA
Cuts at: 472 1279 472
Size: 807 2711
MslI CAynn'nnrTG
Cuts at: 196 373 1507 1634 196
Size: 177 1134 127 2080
Fragments arranged by size:
2080 1134 177 127
MspA1I CmG'CkG
Cuts at: 294 629 943 1303 2316 2561 294
Size: 335 314 360 1013 245 1251
Fragments arranged by size:
1251 1013 360 335 314 245
NaeI GCC'GGC
Cuts at: 322 1699 322
Size: 1377 2141
NarI GG'CG_CC
Cuts at: 949 1197 949
Size: 248 3270
NgoMIV G'CCGG_C
Cuts at: 320 1697 320
Size: 1377 2141
NspI r_CATG'y
Cuts at: 571 1071 1978 571
Size: 500 907 2111
Fragments arranged by size:
2111 907 500
PasI CC'CwG_GG
Cuts at: 177 177
Size: 3518
PciI A'CATG_T
Cuts at: 1974 1974
Size: 3518
PflMI CCAn_nnn'nTGG
Cuts at: 504 504
Size: 3518
PfoI T'CCnGG_A
Cuts at: 503 503
Size: 3518
PmeI GTTT'AAAC
Cuts at: 517 517
Size: 3518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2657 2689 2657
Size: 32 3486
PstI C_TGCA'G
Cuts at: 565 1250 565
Size: 685 2833
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3518
PvuII CAG'CTG
Cuts at: 294 943 1303 294
Size: 649 360 2509
Fragments arranged by size:
2509 649 360
RsrII CG'GwC_CG
Cuts at: 1713 1713
Size: 3518
SacI G_AGCT'C
Cuts at: 407 532 407
Size: 125 3393
SalI G'TCGA_C
Cuts at: 555 555
Size: 3518
SapI GCTCTTCn'nnn_
Cuts at: 1541 1751 1541
Size: 210 3308
SbfI CC_TGCA'GG
Cuts at: 565 565
Size: 3518
ScaI AGT'ACT
Cuts at: 3035 3035
Size: 3518
SfcI C'TryA_G
Cuts at: 33 561 1246 2239 2430 33
Size: 528 685 993 191 1121
Fragments arranged by size:
1121 993 685 528 191
SfoI GGC'GCC
Cuts at: 950 1198 950
Size: 248 3270
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3518
SgrAI Cr'CCGG_yG
Cuts at: 320 320
Size: 3518
SmaI CCC'GGG
Cuts at: 540 540
Size: 3518
SmlI C'TyrA_G
Cuts at: 673 1588 2080 2342 673
Size: 915 492 262 1849
Fragments arranged by size:
1849 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3518
SphI G_CATG'C
Cuts at: 571 1071 571
Size: 500 3018
StyI C'CwwG_G
Cuts at: 194 652 1006 194
Size: 458 354 2706
Fragments arranged by size:
2706 458 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1101 1234 1693 1902 1101
Size: 133 459 209 2717
Fragments arranged by size:
2717 459 209 133
TatI w'GTAC_w
Cuts at: 3033 3033
Size: 3518
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3486
Tth111I GACn'n_nGTC
Cuts at: 1315 1315
Size: 3518
XbaI T'CTAG_A
Cuts at: 549 549
Size: 3518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1013 1013
Size: 3518
XmaI C'CCGG_G
Cuts at: 538 538
Size: 3518
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AvrII
BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI