Restriction map for pF1KB7275
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AviII       BamHI       
BbeI        BbrPI       BbvCI       BglI        BglII       
BlpI        Bpu1102I    BsaAI       BsaHI       BseAI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        ClaI        CpoI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaI        KasI        KspI        
MfeI        MflI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        Tth111I     XbaI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I       2142  GGTACC               G^GTACC
AflII          52  CTTAAG               C^TTAAG
AlwNI        1396  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       572  GAATTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2142  GGTACC               G^GTACC
AspHI        1220  GAGCTC               G[AT]GC[AT]^C
AvaI         2510  CCCGGG               C^[CT]CG[GA]G
AvrII        1837  CCTAGG               C^CTAGG
BalI         2268  TGGCCA               TGG^CCA
BanII        1220  GAGCTC               G[GA]GC[CT]^C
BfrI           52  CTTAAG               C^TTAAG
BlnI         1837  CCTAGG               C^CTAGG
BsaBI         688  GATGTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          313  GAGACC               GGTCTC(1/5)
BsaWI        2379  ACCGGA               [AT]^CCGG[AT]
BsiHKAI      1220  GAGCTC               G[AT]GC[AT]^C
BsmI         1577  GCATTC               GAATGC(1/-1)
BsoBI        2510  CCCGGG               C^[CT]CG[GA]G
BspHI        1236  TCATGA               T^CATGA
BsrFI         206  GCCGGT               [GA]^CCGG[CT]
BstXI         739  CCAGCCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        206  GCCGGT               [GA]^CCGG[CT]
DraII        1200  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI         2268  TGGCCA               [CT]^GGCC[GA]
EarI          886  GAAGAG               CTCTTC(1/4)
EcoO109I     1200  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       179  ATGCAT               ATGCA^T
HincII         12  GTTGAC               GT[CT]^[GA]AC
HindII         12  GTTGAC               GT[CT]^[GA]AC
KpnI         2142  GGTACC               GGTAC^C
Ksp632I       886  GAAGAG               CTCTTC(1/4)
MamI          688  GATGTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        2268  TGGCCA               TGG^CCA
MscI         2268  TGGCCA               TGG^CCA
NsiI          179  ATGCAT               ATGCA^T
PshAI        1861  GACCACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1236  TCATGA               T^CATGA
SacI         1220  GAGCTC               GAGCT^C
ScaI         1747  AGTACT               AGT^ACT
SmaI         2510  CCCGGG               CCC^GGG
SmlI           52  CTTAAG               C^T[CT][GA]AG
XcmI         2467  CCAGAAGCTCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        2510  CCCGGG               C^CCGGG
XmaI         2510  CCCGGG               C^CCGGG
XmnI          572  GAATTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AflIII        565  ACATGT               A^C[GA][CT]GT
             1190  ACATGT              
AlwI          817  GGATC                GGATC(4/5)
             1389  GGATC               
BanI         1632  GGTGCC               G^G[CT][GA]CC
             2142  GGTACC              
BbsI          256  GAAGAC               GAAGAC(2/6)
             1515  GAAGAC              
BcgI          232  CGAGGTGATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1197  CGAAGGGCCTGC        
BclI           98  TGATCA               T^GATCA
             1296  TGATCA              
BfaI         1838  CTAG                 C^TAG
             2132  CTAG                
BmyI          453  GTGCCC               G[AGT]GC[ACT]^C
             1220  GAGCTC              
BpmI         2282  CTCCAG               CTGGAG(16/14)
              196  CTGGAG              
BpuAI         256  GAAGAC               GAAGAC(2/6)
             1515  GAAGAC              
BseRI        1436  GAGGAG               GAGGAG(10/8)
             1660  GAGGAG              
BsgI         1342  CTGCAC               GTGCAG(16/14)
             2563  GTGCAG              
Bsp1286I      453  GTGCCC               G[AGT]GC[ACT]^C
             1220  GAGCTC              
BspLU11I      565  ACATGT               A^CATGT
             1190  ACATGT              
DraI          789  TTTAAA               TTT^AAA
             1507  TTTAAA              
EcoRI         572  GAATTC               G^AATTC
             2158  GAATTC              
FauI         1211  CCCGC                CCCGC(4/6)
             1995  GCGGG               
FbaI           98  TGATCA               T^GATCA
             1296  TGATCA              
MaeI         1838  CTAG                 C^TAG
             2132  CTAG                
PflMI        1116  CCATGGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1591  CCAAACAATGG         
PstI          159  CTGCAG               CTGCA^G
             1846  CTGCAG              
PvuII         156  CAGCTG               CAG^CTG
             1753  CAGCTG              
SfcI          159  CTGCAG               C^T[GA][CT]AG
             1846  CTGCAG              
TaqI          279  TCGA                 T^CGA
             1367  TCGA                
TthHB8I       279  TCGA                 T^CGA
             1367  TCGA                
Van91I       1116  CCATGGAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1591  CCAAACAATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BbvI        BciVI       BcnI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DsaI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SspI        StuI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       
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