Restriction map for pF1KB7275
 (Linear) (Six-base) MAPSORT of: KIBB7275.seq  Check: 4430  from: 1  to: 2625



KIBB7275.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     979    2625 
   Size:        979    1646
 
Acc65I G'GTAC_C

Cuts at:      0    2142    2625 
   Size:       2142     483
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1087    1541    1648    2477    2625 
   Size:       1087     454     107     829     148

  Fragments arranged by size: 

                1087     829     454     148     107
 
AflII C'TTAA_G

Cuts at:      0      52    2625 
   Size:         52    2573
 
AflIII A'CryG_T

Cuts at:      0     565    1190    2625 
   Size:        565     625    1435

  Fragments arranged by size: 

                1435     625     565
 
AleI CACnn'nnGTG

Cuts at:      0     265    1088    2625 
   Size:        265     823    1537

  Fragments arranged by size: 

                1537     823     265
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1401    2625 
   Size:       1401    1224
 
ApoI r'AATT_y

Cuts at:      0     298     572     705    1077    1818    2158    2579    2625 
   Size:        298     274     133     372     741     340     421      46

  Fragments arranged by size: 

                 741     421     372     340     298     274     133      46
            
 
AvaI C'yCGr_G

Cuts at:      0    2510    2625 
   Size:       2510     115
 
AvrII C'CTAG_G

Cuts at:      0    1837    2625 
   Size:       1837     788
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2134    2167    2625 
   Size:       2134      33     458

  Fragments arranged by size: 

                2134     458      33
 
BanI G'GyrC_C

Cuts at:      0    1632    2142    2625 
   Size:       1632     510     483

  Fragments arranged by size: 

                1632     510     483
 
BanII G_rGCy'C

Cuts at:      0    1224    2625 
   Size:       1224    1401
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     263    1522    2625 
   Size:        263    1259    1103

  Fragments arranged by size: 

                1259    1103     263
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     221     255    1186    1220    2625 
   Size:        221      34     931      34    1405

  Fragments arranged by size: 

                1405     931     221      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     525     611     659    2625 
   Size:        525      86      48    1966

  Fragments arranged by size: 

                1966     525      86      48
 
BclI T'GATC_A

Cuts at:      0      98    1296    2625 
   Size:         98    1198    1329

  Fragments arranged by size: 

                1329    1198      98
 
BfrBI ATG'CAT

Cuts at:      0     181    2625 
   Size:        181    2444
 
Bme1580I G_kGCm'C

Cuts at:      0     457    2625 
   Size:        457    2168
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1496    1941    2546    2625 
   Size:       1496     445     605      79

  Fragments arranged by size: 

                1496     605     445      79
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217    2267    2625 
   Size:        217    2050     358

  Fragments arranged by size: 

                2050     358     217
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     307    2625 
   Size:        307    2318
 
BsaBI GATnn'nnATC

Cuts at:      0     692    2625 
   Size:        692    1933
 
BsaWI w'CCGG_w

Cuts at:      0    2379    2625 
   Size:       2379     246
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2216    2246    2625 
   Size:       2216      30     379

  Fragments arranged by size: 

                2216     379      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1451    1675    2625 
   Size:       1451     224     950

  Fragments arranged by size: 

                1451     950     224
 
BseYI C'CCAG_C

Cuts at:      0     245     440    2625 
   Size:        245     195    2185

  Fragments arranged by size: 

                2185     245     195
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1327    2584    2625 
   Size:       1327    1257      41

  Fragments arranged by size: 

                1327    1257      41
 
BsiHKAI G_wGCw'C

Cuts at:      0    1224    2625 
   Size:       1224    1401
 
BsmI GAATG_Cn'

Cuts at:      0    1577    2625 
   Size:       1577    1048
 
Bsp1286I G_dGCh'C

Cuts at:      0     457    1224    2625 
   Size:        457     767    1401

  Fragments arranged by size: 

                1401     767     457
 
BspHI T'CATG_A

Cuts at:      0    1236    2625 
   Size:       1236    1389
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     479     929     979    2625 
   Size:        479     450      50    1646

  Fragments arranged by size: 

                1646     479     450      50
 
BsrDI GCAATG_nn'

Cuts at:      0     782    1699    2504    2625 
   Size:        782     917     805     121

  Fragments arranged by size: 

                 917     805     782     121
 
BsrFI r'CCGG_y

Cuts at:      0     206    2625 
   Size:        206    2419
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     746    2625 
   Size:        746    1879
 
BtgI C'CryG_G

Cuts at:      0    1116    1446    1605    2067    2625 
   Size:       1116     330     159     462     558

  Fragments arranged by size: 

                1116     558     462     330     159
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1946    2625 
   Size:       1946     679
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     745     780    2625 
   Size:        745      35    1845

  Fragments arranged by size: 

                1845     745      35
 
DraI TTT'AAA

Cuts at:      0     791    1509    2625 
   Size:        791     718    1116

  Fragments arranged by size: 

                1116     791     718
 
EaeI y'GGCC_r

Cuts at:      0    2268    2625 
   Size:       2268     357
 
EarI CTCTTCn'nnn_

Cuts at:      0     881    2625 
   Size:        881    1744
 
EcoICRI GAG'CTC

Cuts at:      0    1222    2625 
   Size:       1222    1403
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217    1087    1541    1648    2267    2477    2625 
   Size:        217     870     454     107     619     210     148

  Fragments arranged by size: 

                 870     619     454     217     210     148     107
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1201    2625 
   Size:       1201    1424
 
EcoRI G'AATT_C

Cuts at:      0     572    2158    2625 
   Size:        572    1586     467

  Fragments arranged by size: 

                1586     572     467
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     189    2625 
   Size:        189    2436
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1651    1683    1764    1796    2625 
   Size:       1651      32      81      32     829

  Fragments arranged by size: 

                1651     829      81      32      32
 
HincII GTy'rAC

Cuts at:      0      14    2625 
   Size:         14    2611
 
KpnI G_GTAC'C

Cuts at:      0    2146    2625 
   Size:       2146     479
 
MscI TGG'CCA

Cuts at:      0    2270    2625 
   Size:       2270     355
 
MslI CAynn'nnrTG

Cuts at:      0     265     856     989    1088    2276    2625 
   Size:        265     591     133      99    1188     349

  Fragments arranged by size: 

                1188     591     349     265     133      99
 
MspA1I CmG'CkG

Cuts at:      0     158    1074    1755    2625 
   Size:        158     916     681     870

  Fragments arranged by size: 

                 916     870     681     158
 
NcoI C'CATG_G

Cuts at:      0    1116    1446    1605    2625 
   Size:       1116     330     159    1020

  Fragments arranged by size: 

                1116    1020     330     159
 
NsiI A_TGCA'T

Cuts at:      0     183    2625 
   Size:        183    2442
 
NspI r_CATG'y

Cuts at:      0     224     569     861     988    1194    2209    2625 
   Size:        224     345     292     127     206    1015     416

  Fragments arranged by size: 

                1015     416     345     292     224     206     127
 
PasI CC'CwG_GG

Cuts at:      0    2392    2625 
   Size:       2392     233
 
PciI A'CATG_T

Cuts at:      0     565    1190    2625 
   Size:        565     625    1435

  Fragments arranged by size: 

                1435     625     565
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1122    1597    2625 
   Size:       1122     475    1028

  Fragments arranged by size: 

                1122    1028     475
 
PshAI GACnn'nnGTC

Cuts at:      0    1865    2625 
   Size:       1865     760
 
PsiI TTA'TAA

Cuts at:      0    1454    2200    2258    2625 
   Size:       1454     746      58     367

  Fragments arranged by size: 

                1454     746     367      58
 
PstI C_TGCA'G

Cuts at:      0     163    1850    2625 
   Size:        163    1687     775

  Fragments arranged by size: 

                1687     775     163
 
PvuII CAG'CTG

Cuts at:      0     158    1755    2625 
   Size:        158    1597     870

  Fragments arranged by size: 

                1597     870     158
 
SacI G_AGCT'C

Cuts at:      0    1224    2625 
   Size:       1224    1401
 
ScaI AGT'ACT

Cuts at:      0    1749    2625 
   Size:       1749     876
 
SfcI C'TryA_G

Cuts at:      0     159    1846    2625 
   Size:        159    1687     779

  Fragments arranged by size: 

                1687     779     159
 
SmaI CCC'GGG

Cuts at:      0    2512    2625 
   Size:       2512     113
 
SmlI C'TyrA_G

Cuts at:      0      52    2625 
   Size:         52    2573
 
SspI AAT'ATT

Cuts at:      0     517    1542    2249    2625 
   Size:        517    1025     707     376

  Fragments arranged by size: 

                1025     707     517     376
 
StuI AGG'CCT

Cuts at:      0     119    1064    1836    2625 
   Size:        119     945     772     789

  Fragments arranged by size: 

                 945     789     772     119
 
StyI C'CwwG_G

Cuts at:      0     399    1116    1446    1605    1837    2625 
   Size:        399     717     330     159     232     788

  Fragments arranged by size: 

                 788     717     399     330     232     159
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     245    1801    2080    2384    2625 
   Size:        245    1556     279     304     241

  Fragments arranged by size: 

                1556     304     279     245     241
 
TatI w'GTAC_w

Cuts at:      0    1747    2625 
   Size:       1747     878
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     809     841    1381    1413    2216    2248    2304    2336 
   Size:        809      32     540      32     803      32      56      32

Cuts at:   2336    2625 
   Size:        289

  Fragments arranged by size: 

                 809     803     540     289      56      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2474    2625 
   Size:       2474     151
 
XmaI C'CCGG_G

Cuts at:      0    2510    2625 
   Size:       2510     115
 
XmnI GAAnn'nnTTC

Cuts at:      0     576    2625 
   Size:        576    2049
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AflII   AflIII     AleI    AlwNI     ApoI
     AvaI    AvrII     BaeI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI Bme1580I     BmrI     BpmI     BsaI    BsaBI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrDI    BsrFI    BstXI     BtgI    BtgZI    CspCI     DraI
     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    Hin4I
   HincII     KpnI     MscI     MslI   MspA1I     NcoI     NsiI     NspI
     PasI     PciI    PflMI    PshAI     PsiI     PstI    PvuII     SacI
     ScaI     SfcI     SmaI     SmlI     SspI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI     AgeI     AhdI     AlfI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    BamHI     BbeI    BbvCI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaHI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtsI     ClaI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PfoI     PmeI     PmlI     PpiI    PpuMI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SwaI  Tth111I     XbaI     XhoI     ZraI
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