Restriction map for pF1KB6910
##################################
# NO CUT ENZYMES (  188 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstF5I      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FokI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeII       MaeIII      
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NlaIV       NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfaNI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        Tsp45I      
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AciI           88  GCGG                 CCGC(-3/-1)
AflII         316  CTTAAG               C^TTAAG
AlwI          312  GGATC                GGATC(4/5)
Asp700I        51  GAATAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII         501  GAGCCC               G[GA]GC[CT]^C
BbsI          606  GAAGAC               GAAGAC(2/6)
BbvI          390  GCTGC                GCAGC(8/12)
BfrI          316  CTTAAG               C^TTAAG
BpuAI         606  GAAGAC               GAAGAC(2/6)
BsaJI         659  CCACGG               C^C[ACGT][ACGT]GG
BsiYI         635  CCAATGTTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          635  CCAATGTTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          369  GAATGC               GAATGC(1/-1)
BsrI           15  ACTGG                ACTGG(1/-1)
BssKI         309  CCAGG                ^CC[ACGT]GG
BstBI         437  TTCGAA               TT^CGAA
BstNI         309  CCAGG                CC^[AT]GG
BstYI         312  GGATCT               [GA]^GATC[CT]
Cac8I         206  GCAGGC               GC[ACGT]^[ACGT]GC
Cfr13I        576  GGGCC                G^G[ACGT]CC
DraII         575  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI          659  CCACGG               C^C[GA][CT]GG
EcoO109I      575  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRII        309  CCAGG                ^CC[AT]GG
FauI           88  GCGGG                CCCGC(4/6)
Fnu4HI        390  GCTGC                GC^[ACGT]GC
HaeIII        577  GGCC                 GG^CC
HindIII       647  AAGCTT               A^AGCTT
HphI          451  GGTGA                GGTGA(8/7)
ItaI          390  GCTGC                GC^[ACGT]GC
MflI          312  GGATCT               [GA]^GATC[CT]
MslI          523  CATCTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          309  CCAGG                CC^[AT]GG
NspV          437  TTCGAA               TT^CGAA
Sau96I        576  GGGCC                G^G[ACGT]CC
ScaI          291  AGTACT               AGT^ACT
ScrFI         309  CCAGG                CC^[ACGT]GG
SfuI          437  TTCGAA               TT^CGAA
TseI          390  GCTGC                G^C[AT]GC
XhoII         312  GGATCT               [GA]^GATC[CT]
XmnI           51  GAATAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AfaI           65  GTAC                 GT^AC
              292  GTAC                
BmyI          501  GAGCCC               G[AGT]GC[ACT]^C
              560  GTGCCC              
BsmFI          90  GGGAC                GGGAC(10/14)
              539  GGGAC               
Bsp1286I      501  GAGCCC               G[AGT]GC[ACT]^C
              560  GTGCCC              
HinfI         112  GATTC                G^A[ACGT]TC
              492  GAATC               
PstI          391  CTGCAG               CTGCA^G
              401  CTGCAG              
RsaI           65  GTAC                 GT^AC
              292  GTAC                
SfcI          391  CTGCAG               C^T[GA][CT]AG
              401  CTGCAG              
SmlI          316  CTTAAG               C^T[CT][GA]AG
              571  CTTGAG              
SspI          326  AATATT               AAT^ATT
              478  AATATT              
TfiI          112  GATTC                G^A[AT]TC
              492  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AcsI        AluI        ApoI        BfaI        DdeI        
DpnII       MaeI        MboI        MboII       MnlI        
MseI        MwoI        NdeII       Sau3AI      TaqI        
Tru9I       Tsp509I     TthHB8I     
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