Restriction map for pF1KB7685
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbvI        BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRV       
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       ItaI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MluNI       MroI        MscI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        TseI        
Tth111I     TthHB8I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AciI         1028  CCGC                 CCGC(-3/-1)
Alw44I        305  GTGCAC               G^TGCAC
AlwI          351  GGATC                GGATC(4/5)
AocI           19  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       824  AGCGCT               AGC^GCT
ApaLI         305  GTGCAC               G^TGCAC
BanII         190  GAGCTC               G[GA]GC[CT]^C
BbsI          698  GAAGAC               GAAGAC(2/6)
BpuAI         698  GAAGAC               GAAGAC(2/6)
BsgI           93  GTGCAG               GTGCAG(16/14)
BsmAI         319  GAGAC                GTCTC(1/5)
BstXI           5  CCACAGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         351  GGATCT               [GA]^GATC[CT]
Bsu36I         19  CCTGAGG              CC^T[ACGT]AGG
DraII         386  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EarI          550  GAAGAG               CTCTTC(1/4)
Eco47III      824  AGCGCT               AGC^GCT
Eco81I         19  CCTGAGG              CC^T[ACGT]AGG
EcoNI         437  CCTTGGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      386  AGGTCCC              [GA]G^G[ACGT]CC[CT]
HaeII         824  AGCGCT               [GA]GCGC^[CT]
Ksp632I       550  GAAGAG               CTCTTC(1/4)
MaeIII        337  GTGAC                ^GT[ACGT]AC
MflI          351  GGATCT               [GA]^GATC[CT]
MseI           47  TTAA                 T^TAA
MwoI          183  GCTGTGGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          449  CATATG               CA^TATG
PflMI         316  CCAGAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         386  AGGTCCC              [GA]G^G[AT]CC[CT]
SacI          190  GAGCTC               GAGCT^C
SfcI          117  CTATAG               C^T[GA][CT]AG
StuI          979  AGGCCT               AGG^CCT
Tru9I          47  TTAA                 T^TAA
Tsp45I        337  GTGAC                ^GT[GC]AC
Van91I        316  CCAGAGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          312  CCATCCAGAGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         351  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AcsI          589  GAATTC               [GA]^AATT[CT]
             1019  GAATTC              
AfaI          343  GTAC                 GT^AC
              853  GTAC                
ApoI          589  GAATTC               [GA]^AATT[CT]
             1019  GAATTC              
Asp700I       726  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              894  GAAAGACTTC          
AspHI         190  GAGCTC               G[AT]GC[AT]^C
              305  GTGCAC              
BbvCI         631  CCTCAGC              CCTCAGC(-5/-2)
              207  GCTGAGG             
BfaI          256  CTAG                 C^TAG
              536  CTAG                
BmyI          190  GAGCTC               G[AGT]GC[ACT]^C
              305  GTGCAC              
BseRI         629  CTCCTC               GAGGAG(10/8)
              328  GAGGAG              
BsiHKAI       190  GAGCTC               G[AT]GC[AT]^C
              305  GTGCAC              
BsmI          217  GAATGC               GAATGC(1/-1)
              272  GCATTC              
Bsp1286I      190  GAGCTC               G[AGT]GC[ACT]^C
              305  GTGCAC              
Cac8I         291  GCAAGC               GC[ACGT]^[ACGT]GC
              303  GCGTGC              
CfoI          825  GCGC                 GCG^C
              909  GCGC                
DpnII         352  GATC                 ^GATC
              674  GATC                
EcoRI         589  GAATTC               G^AATTC
             1019  GAATTC              
HhaI          825  GCGC                 GCG^C
              909  GCGC                
HinP1I        825  GCGC                 G^CGC
              909  GCGC                
HphI          526  GGTGA                GGTGA(8/7)
              946  GGTGA               
MaeI          256  CTAG                 C^TAG
              536  CTAG                
MboI          352  GATC                 ^GATC
              674  GATC                
MspA1I        181  CAGCTG               C[AC]G^C[GT]G
              490  CAGCTG              
NdeII         352  GATC                 ^GATC
              674  GATC                
NlaIII        296  CATG                 CATG^
              420  CATG                
PvuII         181  CAGCTG               CAG^CTG
              490  CAGCTG              
RsaI          343  GTAC                 GT^AC
              853  GTAC                
Sau3AI        352  GATC                 ^GATC
              674  GATC                
XmnI          726  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              894  GAAAGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AluI        AlwNI       AvaII       BpmI        BsaJI       
BsiYI       BslI        BsmFI       BspMI       BsrI        
BssKI       BstF5I      BstNI       Cfr13I      DdeI        
EcoRII      EcoT14I     FokI        HaeIII      HinfI       
MboII       MnlI        MslI        MvaI        NlaIV       
Sau96I      ScrFI       SfaNI       StyI        TfiI        
Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com